FastQCFastQC Report
Thu 2 Feb 2017
SRR4061966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31956
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA5371.6804355989485542No Hit
GTATCAACGCAGAGTACTTTTTTTT4001.251721116535236No Hit
TATCAACGCAGAGTACTTTTTTTTT3241.0138941043935412No Hit
GGTATCAACGCAGAGTACTTTTTTT2350.7353861559644511No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2000.625860558267618No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1940.6070847415195894No Hit
GTACATGGGGTGGTATCAACGCAAA1670.522593566153461No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1400.43810239078733254No Hit
GTATCAACGCAGAGTACATGGGGTG1220.38177494054324695No Hit
TATCAACGCAGAGTACATGGGGTGG840.26286143447239957No Hit
GGTATCAACGCAGAGTACATGGGGT700.21905119539366627No Hit
GCAGAGTACTTTTTTTTTTTTTTTT600.1877581674802854No Hit
ATCAACGCAGAGTACTTTTTTTTTT550.17211165352359495No Hit
GTACATGGGTGGTATCAACGCAAAA520.16272374514958068No Hit
ACGCAGAGTACATGGGGTGGTATCA510.15959444235824258No Hit
CATCAGGATCGGTCGATGGTGCAGG480.1502065339842283No Hit
CAAGTAGAGTGATCGAAAGATGAAA450.14081862561021405No Hit
GGCTATAACACTCCCCGAGGGGAGC450.14081862561021405No Hit
GTATCAACGCAGAGTACATGGGCAG420.13143071723619978No Hit
GTATCAACGCAGAGTACATGGGTGG410.12830141444486168No Hit
GTTATAGCCTGTGGTGATGCGGCGC400.1251721116535236No Hit
GTGCTGGAAGGCGCCGGCCGTGAAG350.10952559769683314No Hit
GTCCTAGACAGTCCTTGAAAAACCG340.10639629490549506No Hit
GGTATCAACGCAGAGTACATGGGCA340.10639629490549506No Hit
GTATCAACGCAGAGTACATGGGAGT320.10013768932281887No Hit
ATTCCATTCCATTCCATTCCATTCC320.10013768932281887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAA657.6900714E-411.68556619
GGGGTGG751.9373368E-411.4112857
AACGCAA700.001430256310.850883518
TGGGGTG803.5250554E-410.698086
GGTGGTA803.5250554E-410.698089
GGGTGGT1002.5445997E-49.5094048
ATGGGGT950.00173302058.9948115
TGGTATC1050.00423290958.15091811
GTGGTAT1050.00423290958.15091810
CATGGGG1409.511331E-58.1381634
ACATGGG3009.0221874E-107.27913433
TACATGG3009.0221874E-107.27913432
GGTATCA1905.6424295E-56.99596451
GTACATG3007.1213435E-96.96265031
GTATCAA2704.3401815E-76.6813311
AACGCAG2754.152511E-66.22433766
TATCAAC3207.995181E-65.6373732
ATCAACG3251.0386362E-55.5506443
TCAACGC3301.3428991E-55.46654374
CAACGCA3351.728348E-55.3849535
Produced by FastQC (version 0.11.5)