##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061966_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31956
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.53739516835649	32.0	32.0	32.0	32.0	32.0
2	30.20484416072099	32.0	32.0	32.0	21.0	32.0
3	30.405933158092378	32.0	32.0	32.0	32.0	32.0
4	30.236919514332207	32.0	32.0	32.0	21.0	32.0
5	30.362467142320693	32.0	32.0	32.0	21.0	32.0
6	33.84600700963825	36.0	36.0	36.0	32.0	36.0
7	33.64942420828639	36.0	36.0	36.0	21.0	36.0
8	33.454437351358116	36.0	36.0	36.0	21.0	36.0
9	33.39939291525848	36.0	36.0	36.0	21.0	36.0
10	33.324258355238456	36.0	36.0	36.0	21.0	36.0
11	33.743397171110274	36.0	36.0	36.0	27.0	36.0
12	33.523094254600075	36.0	36.0	36.0	21.0	36.0
13	33.620165227187385	36.0	36.0	36.0	27.0	36.0
14	33.4777819501815	36.0	36.0	36.0	21.0	36.0
15	33.59885467517837	36.0	36.0	36.0	27.0	36.0
16	33.53001001376893	36.0	36.0	36.0	21.0	36.0
17	33.42827638002253	36.0	36.0	36.0	21.0	36.0
18	33.565746651646016	36.0	36.0	36.0	27.0	36.0
19	33.42643009137564	36.0	36.0	36.0	21.0	36.0
20	33.23260107648016	36.0	36.0	36.0	21.0	36.0
21	33.2247465264739	36.0	36.0	36.0	21.0	36.0
22	33.274752785079485	36.0	36.0	36.0	21.0	36.0
23	33.27763174364752	36.0	36.0	36.0	21.0	36.0
24	33.262642383277004	36.0	36.0	36.0	21.0	36.0
25	32.96429465515083	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	4.0
7	3.0
8	5.0
9	4.0
10	2.0
11	0.0
12	1.0
13	3.0
14	47.0
15	48.0
16	39.0
17	68.0
18	92.0
19	116.0
20	185.0
21	233.0
22	234.0
23	336.0
24	382.0
25	447.0
26	564.0
27	628.0
28	768.0
29	940.0
30	1176.0
31	1515.0
32	2111.0
33	2883.0
34	6005.0
35	13116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59849788765452	20.372398685651696	11.847911125019559	22.18119230167423
2	16.150331706095884	21.082112905244713	39.3447239954938	23.422831393165602
3	19.257729377894606	26.855676555263486	28.11678558017274	25.76980848666917
4	12.088496682939041	16.83877832019026	32.87958442858931	38.193140568281386
5	13.108391498951455	39.09042536542614	31.133994804219224	16.667188331403175
6	29.77584371673658	37.906204996556255	16.182455700957988	16.135495585749172
7	27.89575894255466	34.02242686211865	18.759005199523898	19.32280899580279
8	28.478356198709516	32.78519075361774	17.932092964981518	20.804360082691222
9	27.59171653247282	15.282433660202388	19.32391365644287	37.80193615088192
10	18.784270719097602	27.48864170452765	26.529844900517	27.197242675857748
11	33.58403058506471	25.32355614051581	20.434959731753942	20.65745354266554
12	23.694187686041047	26.727244242519195	27.45417515274949	22.124392918690273
13	29.292897647169397	22.250070490930167	26.069112440865943	22.387919421034493
14	22.98342887573223	21.486075870062336	25.633555743507813	29.896939510697617
15	25.354848817170613	27.6672411091963	23.34952216825944	23.62838790537365
16	22.720010025376737	29.10492183339077	23.559635326921267	24.615432814311227
17	20.436103887966414	28.55039318274382	25.912465929383753	25.10103699990601
18	20.835160704216527	28.44120042603847	27.357308439320843	23.36633043042416
19	24.2841927197544	25.186391830085835	27.817805901885844	22.711609548273916
20	25.041495725157368	24.477780213585543	27.531239234599603	22.94948482665748
21	26.00294384767154	23.945382230434372	27.36838808681219	22.683285835081897
22	24.553711243344818	25.339805825242717	27.237707485123707	22.868775446288755
23	23.559258331245303	26.6036081182661	27.349035329491358	22.488098220997244
24	23.632947071719386	26.38584403382399	27.2471030378954	22.734105856561225
25	22.96201183802574	26.316119131878114	27.340202311233597	23.381666718862547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	577.0
1	577.0
2	341.0
3	105.0
4	105.0
5	105.0
6	183.5
7	262.0
8	262.0
9	262.0
10	254.0
11	246.0
12	246.0
13	246.0
14	182.5
15	119.0
16	119.0
17	119.0
18	167.5
19	216.0
20	216.0
21	216.0
22	373.0
23	530.0
24	530.0
25	530.0
26	716.0
27	902.0
28	902.0
29	902.0
30	1079.5
31	1257.0
32	1257.0
33	1257.0
34	1450.0
35	1643.0
36	1643.0
37	1643.0
38	1905.5
39	2168.0
40	2168.0
41	2168.0
42	2696.0
43	3224.0
44	3224.0
45	3224.0
46	3894.0
47	4564.0
48	4564.0
49	4564.0
50	4774.5
51	4985.0
52	4985.0
53	4985.0
54	4465.5
55	3946.0
56	3946.0
57	3946.0
58	3544.5
59	3143.0
60	3143.0
61	3143.0
62	2513.0
63	1883.0
64	1883.0
65	1883.0
66	1523.5
67	1164.0
68	1164.0
69	1164.0
70	887.5
71	611.0
72	611.0
73	611.0
74	445.0
75	279.0
76	279.0
77	279.0
78	182.5
79	86.0
80	86.0
81	86.0
82	58.0
83	30.0
84	30.0
85	30.0
86	16.5
87	3.0
88	3.0
89	3.0
90	3.5
91	4.0
92	4.0
93	4.0
94	3.0
95	2.0
96	2.0
97	2.0
98	4.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031293027913380897
2	0.0
3	0.0
4	0.0
5	0.02190511953936663
6	0.04381023907873326
7	0.0938790837401427
8	0.0938790837401427
9	0.11578420327950933
10	0.12830141444486168
11	0.14081862561021405
12	0.12830141444486168
13	0.11578420327950933
14	0.10326699211415696
15	0.12830141444486168
16	0.11578420327950933
17	0.11578420327950933
18	0.10639629490549506
19	0.10639629490549506
20	0.07823256978345225
21	0.07823256978345225
22	0.08136187257479034
23	0.08762047815746651
24	0.08136187257479034
25	0.07823256978345225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	31956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.876455125797975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28494187618276	42.99349105019402
2	14.944579616112463	17.298785830516962
3	5.223033252230333	9.068719489297784
4	2.1303054879697214	4.93178119914883
5	1.1624763449580968	3.3640005006884466
6	0.5298729386320626	1.840030041306797
7	0.32441200324412006	1.3143071723619977
8	0.259529602595296	1.2016522718738265
9	0.20005406866720737	1.0420578295155838
>10	0.8596918085969182	8.524220803604956
>50	0.032441200324412	1.1641006383777694
>100	0.04325493376588267	5.576417574164476
>500	0.005406866720735334	1.6804355989485542
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	537	1.6804355989485542	No Hit
GTATCAACGCAGAGTACTTTTTTTT	400	1.251721116535236	No Hit
TATCAACGCAGAGTACTTTTTTTTT	324	1.0138941043935412	No Hit
GGTATCAACGCAGAGTACTTTTTTT	235	0.7353861559644511	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	200	0.625860558267618	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	194	0.6070847415195894	No Hit
GTACATGGGGTGGTATCAACGCAAA	167	0.522593566153461	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	140	0.43810239078733254	No Hit
GTATCAACGCAGAGTACATGGGGTG	122	0.38177494054324695	No Hit
TATCAACGCAGAGTACATGGGGTGG	84	0.26286143447239957	No Hit
GGTATCAACGCAGAGTACATGGGGT	70	0.21905119539366627	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	60	0.1877581674802854	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	55	0.17211165352359495	No Hit
GTACATGGGTGGTATCAACGCAAAA	52	0.16272374514958068	No Hit
ACGCAGAGTACATGGGGTGGTATCA	51	0.15959444235824258	No Hit
CATCAGGATCGGTCGATGGTGCAGG	48	0.1502065339842283	No Hit
CAAGTAGAGTGATCGAAAGATGAAA	45	0.14081862561021405	No Hit
GGCTATAACACTCCCCGAGGGGAGC	45	0.14081862561021405	No Hit
GTATCAACGCAGAGTACATGGGCAG	42	0.13143071723619978	No Hit
GTATCAACGCAGAGTACATGGGTGG	41	0.12830141444486168	No Hit
GTTATAGCCTGTGGTGATGCGGCGC	40	0.1251721116535236	No Hit
GTGCTGGAAGGCGCCGGCCGTGAAG	35	0.10952559769683314	No Hit
GTCCTAGACAGTCCTTGAAAAACCG	34	0.10639629490549506	No Hit
GGTATCAACGCAGAGTACATGGGCA	34	0.10639629490549506	No Hit
GTATCAACGCAGAGTACATGGGAGT	32	0.10013768932281887	No Hit
ATTCCATTCCATTCCATTCCATTCC	32	0.10013768932281887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAAA	65	7.6900714E-4	11.685566	19
GGGGTGG	75	1.9373368E-4	11.411285	7
AACGCAA	70	0.0014302563	10.8508835	18
TGGGGTG	80	3.5250554E-4	10.69808	6
GGTGGTA	80	3.5250554E-4	10.69808	9
GGGTGGT	100	2.5445997E-4	9.509404	8
ATGGGGT	95	0.0017330205	8.994811	5
TGGTATC	105	0.0042329095	8.150918	11
GTGGTAT	105	0.0042329095	8.150918	10
CATGGGG	140	9.511331E-5	8.138163	4
ACATGGG	300	9.0221874E-10	7.2791343	3
TACATGG	300	9.0221874E-10	7.2791343	2
GGTATCA	190	5.6424295E-5	6.9959645	1
GTACATG	300	7.1213435E-9	6.9626503	1
GTATCAA	270	4.3401815E-7	6.681331	1
AACGCAG	275	4.152511E-6	6.2243376	6
TATCAAC	320	7.995181E-6	5.637373	2
ATCAACG	325	1.0386362E-5	5.550644	3
TCAACGC	330	1.3428991E-5	5.4665437	4
CAACGCA	335	1.728348E-5	5.384953	5
>>END_MODULE