FastQCFastQC Report
Thu 2 Feb 2017
SRR4061966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31956
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.7791963950431844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.5538865940668419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.42871448241331833No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.419326574039304No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.3004130679684566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.25660282888972336No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA700.21905119539366627No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.19401677306296156No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA520.16272374514958068No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.14394792840155213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.14081862561021405No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.13456002002753786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.13143071723619978No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA410.12830141444486168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.11891350607084741No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA380.11891350607084741No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT370.11578420327950933No Hit
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC360.11265490048817123No Hit
ATCCTAAGCCATAGGGAAATTCCGTTTTAAAGTGCACACTCCGTGCCGCC360.11265490048817123No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT330.10326699211415696No Hit
GTGCACACTCCGTGCCGCCCGGCGAAAGGGAAACCGGTTAACATTCCGGT330.10326699211415696No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.10326699211415696No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG330.10326699211415696No Hit
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10013768932281887No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA320.10013768932281887No Hit
GGCTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCG320.10013768932281887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG250.002318783735.210
ATGGATT300.005662766329.33333217
ATACATG300.005662766329.3333321
CATGGGG902.1316562E-417.111114
TACATGG2500.015.842
ACATGGG2600.015.230773
GTACATG2206.5434506E-813.01
CGCAGAG3650.012.6575347
CAGAGTA3700.012.4864869
AGAGTAC3700.012.48648610
GCAGAGT3700.012.4864868
AACGCAG3750.012.3200015
ACGCAGA3750.012.3200016
GGTATCA2004.318332E-612.11
ATCAACG3950.011.6962022
GTATCAA2655.7556463E-811.6226421
TATCAAC3855.456968E-1211.4285721
AGTACAT1559.783811E-411.35483812
TCAACGC4201.8189894E-1210.9999993
GAGTACA1650.001649923710.66666711
Produced by FastQC (version 0.11.5)