##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31956
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.806890724746527	32.0	32.0	32.0	32.0	32.0
2	31.208599324070597	32.0	32.0	32.0	32.0	32.0
3	31.46085242208036	32.0	32.0	32.0	32.0	32.0
4	31.63058580548254	32.0	32.0	32.0	32.0	32.0
5	31.580923770184004	32.0	32.0	32.0	32.0	32.0
6	35.17968456627863	36.0	36.0	36.0	36.0	36.0
7	35.20396795593942	36.0	36.0	36.0	36.0	36.0
8	35.129334084366	36.0	36.0	36.0	36.0	36.0
9	35.141945174615095	36.0	36.0	36.0	36.0	36.0
10	35.07306922017774	36.0	36.0	36.0	36.0	36.0
11	35.16779321567155	36.0	36.0	36.0	36.0	36.0
12	35.14676430091376	36.0	36.0	36.0	36.0	36.0
13	35.134153210664664	36.0	36.0	36.0	36.0	36.0
14	35.075134560020025	36.0	36.0	36.0	36.0	36.0
15	35.11437601702341	36.0	36.0	36.0	36.0	36.0
16	35.12977218675679	36.0	36.0	36.0	36.0	36.0
17	35.08430341719865	36.0	36.0	36.0	36.0	36.0
18	35.09685192139192	36.0	36.0	36.0	36.0	36.0
19	35.04728376517712	36.0	36.0	36.0	36.0	36.0
20	34.97346351232945	36.0	36.0	36.0	36.0	36.0
21	34.90393040430592	36.0	36.0	36.0	36.0	36.0
22	34.95690950056328	36.0	36.0	36.0	36.0	36.0
23	34.982382025284764	36.0	36.0	36.0	36.0	36.0
24	34.95271623482288	36.0	36.0	36.0	36.0	36.0
25	34.936162223056705	36.0	36.0	36.0	36.0	36.0
26	34.90834272124171	36.0	36.0	36.0	32.0	36.0
27	34.966422581048946	36.0	36.0	36.0	36.0	36.0
28	34.957660533233195	36.0	36.0	36.0	36.0	36.0
29	34.91575916885718	36.0	36.0	36.0	32.0	36.0
30	34.847884591313054	36.0	36.0	36.0	32.0	36.0
31	34.831706095881835	36.0	36.0	36.0	32.0	36.0
32	34.80341719864814	36.0	36.0	36.0	32.0	36.0
33	34.68052947803229	36.0	36.0	36.0	32.0	36.0
34	34.569251470772315	36.0	36.0	36.0	32.0	36.0
35	34.56668544248341	36.0	36.0	36.0	32.0	36.0
36	34.518181249217676	36.0	36.0	36.0	32.0	36.0
37	34.49565026912004	36.0	36.0	36.0	32.0	36.0
38	34.47934660157717	36.0	36.0	36.0	32.0	36.0
39	34.38696958317687	36.0	36.0	36.0	32.0	36.0
40	34.36368757040931	36.0	36.0	36.0	32.0	36.0
41	34.295406183502315	36.0	36.0	36.0	32.0	36.0
42	34.23785830516961	36.0	36.0	36.0	32.0	36.0
43	34.13646889473026	36.0	36.0	36.0	32.0	36.0
44	33.96801852547252	36.0	36.0	36.0	32.0	36.0
45	33.89507447740643	36.0	36.0	36.0	32.0	36.0
46	33.68794592564777	36.0	36.0	36.0	27.0	36.0
47	33.502753786456374	36.0	36.0	36.0	21.0	36.0
48	33.37166729252723	36.0	36.0	36.0	21.0	36.0
49	33.296501439479286	36.0	36.0	36.0	14.0	36.0
50	32.86747402678683	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	5.0
23	16.0
24	21.0
25	71.0
26	140.0
27	261.0
28	448.0
29	701.0
30	942.0
31	1229.0
32	1775.0
33	2754.0
34	5087.0
35	18504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00964217512444	20.40509657828006	13.630529380458942	21.954731866136555
2	15.899987482788836	20.240330454374767	39.43234447365127	24.42733758918513
3	18.86969583176868	25.757291275503817	29.199524345975718	26.173488546751784
4	12.104143196895732	16.0533233195644	34.053072975341095	37.78946050819877
5	12.611090249092502	37.53285767930905	32.40080110151458	17.455250970083867
6	30.692201777443984	36.55338590562023	16.109650769808486	16.644761547127302
7	27.80072599824759	32.6323695080736	20.16522718738265	19.401677306296158
8	28.39216422581049	32.06596570284141	18.53173113030417	21.010138941043934
9	27.587933408436598	12.964701464513706	20.055701589685818	39.391663537363875
10	18.53173113030417	25.95130804856678	28.32331956440105	27.193641256728
11	34.25334835398674	23.68882213042934	21.222931530854925	20.834897984729004
12	24.267743146826888	25.059456753035427	28.364000500688448	22.30879959944924
13	30.122668669420456	20.597070972587307	27.21554637626737	22.06471398172487
14	23.497934660157714	19.44548754537489	27.506571535861813	29.55000625860558
15	25.83865314807861	25.237827012141693	25.416197271247963	23.50732256853173
16	22.496557766929527	27.365752910251594	25.03442233070472	25.103266992114158
17	20.38114907998498	26.57403930404306	27.58480410564526	25.460007510326697
18	21.34810364250845	24.712104143196896	30.604581299286522	23.335210915008137
19	24.02991613468519	23.04731505820503	30.814244586306174	22.108524220803606
20	24.374139441732382	22.03967955939417	31.01451996495181	22.571661033921643
21	25.231568406559017	21.13218174990612	31.005132056577793	22.631117786957066
22	24.33971711102766	22.49029916134685	31.030166478908498	22.139817248716987
23	22.947177368882212	23.122418325197145	31.706095881837527	22.224308424083112
24	22.93537787513691	23.55812861836958	30.74949147238304	22.757002034110467
25	22.83452246839404	23.094254600075104	30.96132181749906	23.109901114031793
26	21.942671172862685	23.635623982976593	31.145950682188005	23.27575416197271
27	21.24483665039429	23.379021154086868	32.93278257604206	22.44335961947678
28	21.767430216547755	23.93916635373639	31.562147953435975	22.731255476279884
29	21.498310176492677	23.519839779697083	31.850043810239082	23.13180623357116
30	21.535861809988734	24.499311553385905	30.88308924771561	23.08173738890975
31	22.045938164976846	23.992364501189133	30.7673050444361	23.19439228939792
32	21.06959569407936	24.627612967830768	31.183502315684063	23.11928902240581
33	22.612341970209037	23.68882213042934	31.377519088747025	22.321316810614594
34	22.35886844411065	23.438477907122294	30.94254600075103	23.26010764801602
35	21.858179997496556	24.101890098885967	30.369883589936165	23.67004631368131
36	21.726749280260357	24.90299161346852	30.6108399048692	22.75941920140193
37	22.534109400425585	25.103266992114158	29.625109525597697	22.73751408186256
38	22.543497308799598	25.391162848917258	30.676555263487295	21.388784578795843
39	22.262556720388048	24.168361758723204	30.862149898294476	22.706931622594272
40	21.92389535611466	24.759043685066967	30.188384028038552	23.128676930779825
41	22.405807985980726	25.760420578295157	29.831643509826012	22.00212792589811
42	21.36821681166677	26.22519872316455	29.548726294047693	22.857858171120988
43	21.833828821780628	25.90830855891097	29.047097480832416	23.21076513847598
44	21.00832446642048	26.003004318708143	29.96182011641735	23.026851098454028
45	22.681186631618473	25.857428964826635	28.21379396670422	23.247590436850672
46	22.778195018149955	26.70234071848792	26.855676555263486	23.663787708098635
47	22.03967955939417	27.234322193015398	27.309425460007507	23.416572787582925
48	22.60749827877574	26.991925893471862	26.76034299305251	23.64023283469988
49	22.64121420747927	27.128774839618213	27.385385698638707	22.84462525426381
50	21.864438603079233	27.234322193015398	27.7193641256728	23.18187507823257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	127.0
2	21.0
3	78.5
4	136.0
5	111.0
6	86.0
7	60.5
8	35.0
9	58.0
10	81.0
11	123.5
12	166.0
13	287.5
14	409.0
15	518.5
16	628.0
17	658.5
18	689.0
19	625.0
20	561.0
21	534.5
22	508.0
23	410.0
24	312.0
25	265.5
26	219.0
27	207.0
28	195.0
29	219.0
30	243.0
31	347.0
32	451.0
33	529.5
34	608.0
35	617.5
36	627.0
37	693.5
38	760.0
39	913.5
40	1067.0
41	1215.5
42	1364.0
43	1510.0
44	1656.0
45	1810.0
46	1964.0
47	1981.0
48	1998.0
49	2206.0
50	2414.0
51	2493.0
52	2572.0
53	2512.5
54	2453.0
55	2376.5
56	2300.0
57	2108.0
58	1916.0
59	1820.5
60	1725.0
61	1504.0
62	1283.0
63	1006.5
64	730.0
65	661.5
66	593.0
67	488.5
68	384.0
69	298.5
70	213.0
71	177.0
72	141.0
73	111.0
74	81.0
75	79.5
76	78.0
77	55.0
78	32.0
79	25.0
80	18.0
81	9.5
82	1.0
83	2.5
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04068093628739517
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0031293027913380897
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0031293027913380897
40	0.0
41	0.0
42	0.006258605582676179
43	0.0031293027913380897
44	0.006258605582676179
45	0.0
46	0.0
47	0.0
48	0.006258605582676179
49	0.0031293027913380897
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	31956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.28113656277382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78935811673391	45.83176868193766
2	14.737272123780832	18.062335711603456
3	5.075831077975796	9.331580923770185
4	2.032375019149262	4.981850043810239
5	1.1029974978297503	3.3796470146451374
6	0.6485216769647143	2.3845287269996245
7	0.3166011336363172	1.358117411440731
8	0.229791145381198	1.1265490048817124
9	0.1378746872287188	0.7604205782951559
>10	0.8834192922432722	9.46927024658906
>50	0.025532349486799777	1.1328076104643885
>100	0.02042587958943982	2.181124045562649
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.7791963950431844	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.5538865940668419	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.42871448241331833	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134	0.419326574039304	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.3004130679684566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.25660282888972336	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	70	0.21905119539366627	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.19401677306296156	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	52	0.16272374514958068	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46	0.14394792840155213	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.14081862561021405	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.13456002002753786	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	42	0.13143071723619978	No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA	41	0.12830141444486168	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	38	0.11891350607084741	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	38	0.11891350607084741	No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT	37	0.11578420327950933	No Hit
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC	36	0.11265490048817123	No Hit
ATCCTAAGCCATAGGGAAATTCCGTTTTAAAGTGCACACTCCGTGCCGCC	36	0.11265490048817123	No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT	33	0.10326699211415696	No Hit
GTGCACACTCCGTGCCGCCCGGCGAAAGGGAAACCGGTTAACATTCCGGT	33	0.10326699211415696	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.10326699211415696	No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG	33	0.10326699211415696	No Hit
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.10013768932281887	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	32	0.10013768932281887	No Hit
GGCTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCG	32	0.10013768932281887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0031293027913380897	0.0
24	0.0	0.0	0.0	0.006258605582676179	0.0
25	0.0	0.0	0.0	0.006258605582676179	0.0
26	0.0	0.0	0.0	0.01564651395669045	0.0
27	0.0	0.0	0.0	0.01877581674802854	0.0
28	0.0	0.0	0.0	0.02190511953936663	0.0
29	0.0	0.0	0.0	0.02816372512204281	0.0
30	0.0	0.0	0.0	0.05632745024408562	0.0
31	0.0	0.0	0.0	0.0907497809488046	0.0
32	0.0	0.0	0.0	0.13456002002753786	0.0
33	0.0	0.0	0.0	0.16585304794091876	0.0
34	0.0	0.0	0.0	0.1814995618976092	0.0
35	0.0	0.0	0.0	0.2159218926023282	0.0
36	0.0	0.0	0.0	0.26286143447239957	0.0
37	0.0	0.0	0.0	0.2972837651771185	0.0
38	0.0	0.0	0.0	0.3598698210038803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGCCG	25	0.0023187837	35.2	10
ATGGATT	30	0.0056627663	29.333332	17
ATACATG	30	0.0056627663	29.333332	1
CATGGGG	90	2.1316562E-4	17.11111	4
TACATGG	250	0.0	15.84	2
ACATGGG	260	0.0	15.23077	3
GTACATG	220	6.5434506E-8	13.0	1
CGCAGAG	365	0.0	12.657534	7
CAGAGTA	370	0.0	12.486486	9
AGAGTAC	370	0.0	12.486486	10
GCAGAGT	370	0.0	12.486486	8
AACGCAG	375	0.0	12.320001	5
ACGCAGA	375	0.0	12.320001	6
GGTATCA	200	4.318332E-6	12.1	1
ATCAACG	395	0.0	11.696202	2
GTATCAA	265	5.7556463E-8	11.622642	1
TATCAAC	385	5.456968E-12	11.428572	1
AGTACAT	155	9.783811E-4	11.354838	12
TCAACGC	420	1.8189894E-12	10.999999	3
GAGTACA	165	0.0016499237	10.666667	11
>>END_MODULE