FastQCFastQC Report
Thu 2 Feb 2017
SRR4061965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93596
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14331.5310483353989486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11541.2329586734475833No Hit
TATCAACGCAGAGTACTTTTTTTTT9050.9669216633189451No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6580.7030214966451558No Hit
GGTATCAACGCAGAGTACTTTTTTT5630.6015214325398521No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4610.4925424163425787No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4310.4604897645198513No Hit
GTACATGGGGTGGTATCAACGCAAA4080.4359160647890935No Hit
GTATCAACGCAGAGTACATGGGGTG2900.30984230095303217No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2370.253215949399547No Hit
TATCAACGCAGAGTACATGGGGTGG1960.20941065857515276No Hit
GTACATGGGTGGTATCAACGCAAAA1790.19124748920894055No Hit
ATCAACGCAGAGTACTTTTTTTTTT1670.17842642847984955No Hit
GGTATCAACGCAGAGTACATGGGGT1650.17628958502500106No Hit
CAAGTAGAGTGATCGAAAGATGAAA1230.13141587247318262No Hit
ACGCAGAGTACATGGGGTGGTATCA1210.1292790290183341No Hit
GTATCAACGCAGAGTACATGGGCAG1170.12500534210863712No Hit
GTATCAACGCAGAGTACATGGGTGG1110.11859481174409162No Hit
GGTTAAGGTTGTTTCAACCCCAAGG1040.11111585965212188No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1030.11004743792469765No Hit
GTTATAGCCTGTGGTGATGCGGCGC1000.10684217274242488No Hit
ATCCTAAGCCATAGGGAAATTCCGT940.10043164237787941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGTG307.6211226E-419.0101815
GTTTCAA359.812924E-519.011
AGGTTGT453.4657598E-516.897946
TGTTTCA402.715263E-416.6339110
GGTTAAG402.726404E-416.6251
TTAAGGT402.726404E-416.6253
GTTAAGG402.726404E-416.6252
AACCCCA402.7375843E-416.616116
TCAACCC402.7375843E-416.616114
CCCAAGG402.7375843E-416.616119
TTTCAAC508.57499E-515.20000112
TTCAACC508.614329E-515.19186313
CCCCAAG508.614329E-515.19186318
TTGTTTC456.6513725E-414.7856979
TAAGGTT456.6513725E-414.7856974
AAGGTTG551.9205164E-413.8255885
TACTTGT500.00147523813.3071284
GTTGTTT500.00147523813.3071288
CAACCCC550.00304367312.08443715
ACCCCAA550.00304367312.08443717
Produced by FastQC (version 0.11.5)