FastQCFastQC Report
Thu 2 Feb 2017
SRR4061965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93596
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.6560109406384889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5780.6175477584512159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.3985213043292448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.37287918287106286No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.3109107226804564No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1970.21047908030257706No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.20513697166545578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.20193170648318304No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1680.17949485020727382No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.1752211632975768No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1250.1335527159280311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1240.13248429420060687No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA1180.12607376383606136No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.11432112483439465No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG1070.11432112483439465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1060.11325270310697039No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT960.10256848583272789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG452.3121174E-839.110161
GTCTAGA301.2921623E-436.66577539
TGTCTAG301.2921623E-436.66577538
ACAGTGC250.002342028335.1991468
TTATAGC250.002342028335.1991464
TAGATGA353.1958497E-431.42780742
ACATGGT300.005718986529.3326210
TGGGTGG554.898073E-627.999326
TCTAGAT406.984414E-427.49933240
GGCATAG406.984414E-427.4993326
ATAGTTC406.984414E-427.4993329
CTGTCTA450.001388802424.4438537
CATAGTT450.001388802424.443858
AGGCATA450.001388802424.443855
CTTCTAG450.001388802424.443851
GCATAGT450.001388802424.443857
TTCAGGC551.5786094E-423.9994162
ATATCAA651.7906432E-523.6917321
GAGTGAT500.002563209321.9994667
ACTTCCA500.002563209321.99946616