##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93596
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81721441087226	32.0	32.0	32.0	32.0	32.0
2	31.221558613615965	32.0	32.0	32.0	32.0	32.0
3	31.4499337578529	32.0	32.0	32.0	32.0	32.0
4	31.607216120347022	32.0	32.0	32.0	32.0	32.0
5	31.553827086627635	32.0	32.0	32.0	32.0	32.0
6	35.13784777127228	36.0	36.0	36.0	36.0	36.0
7	35.11120133339032	36.0	36.0	36.0	36.0	36.0
8	35.07577246890893	36.0	36.0	36.0	36.0	36.0
9	35.10624385657507	36.0	36.0	36.0	36.0	36.0
10	35.02191332962947	36.0	36.0	36.0	36.0	36.0
11	35.1501987264413	36.0	36.0	36.0	36.0	36.0
12	35.068795675028845	36.0	36.0	36.0	36.0	36.0
13	35.079031155177574	36.0	36.0	36.0	36.0	36.0
14	35.00142100089747	36.0	36.0	36.0	36.0	36.0
15	35.0627590922689	36.0	36.0	36.0	36.0	36.0
16	35.06489593572375	36.0	36.0	36.0	36.0	36.0
17	35.01929569639728	36.0	36.0	36.0	36.0	36.0
18	35.02150732937305	36.0	36.0	36.0	36.0	36.0
19	34.96360955596393	36.0	36.0	36.0	36.0	36.0
20	34.897281935125434	36.0	36.0	36.0	32.0	36.0
21	34.846991324415576	36.0	36.0	36.0	32.0	36.0
22	34.88502713791188	36.0	36.0	36.0	32.0	36.0
23	34.92041326552417	36.0	36.0	36.0	32.0	36.0
24	34.89736740886363	36.0	36.0	36.0	32.0	36.0
25	34.89443993333048	36.0	36.0	36.0	32.0	36.0
26	34.8544061712039	36.0	36.0	36.0	32.0	36.0
27	34.88412966366084	36.0	36.0	36.0	32.0	36.0
28	34.85382922347109	36.0	36.0	36.0	32.0	36.0
29	34.8032608231121	36.0	36.0	36.0	32.0	36.0
30	34.77259711953502	36.0	36.0	36.0	32.0	36.0
31	34.7647228514039	36.0	36.0	36.0	32.0	36.0
32	34.693565964357454	36.0	36.0	36.0	32.0	36.0
33	34.601414590367106	36.0	36.0	36.0	32.0	36.0
34	34.52175306637036	36.0	36.0	36.0	32.0	36.0
35	34.48996751997949	36.0	36.0	36.0	32.0	36.0
36	34.4823710414975	36.0	36.0	36.0	32.0	36.0
37	34.46852429591008	36.0	36.0	36.0	32.0	36.0
38	34.42406726783196	36.0	36.0	36.0	32.0	36.0
39	34.35832727894354	36.0	36.0	36.0	32.0	36.0
40	34.313378776870806	36.0	36.0	36.0	32.0	36.0
41	34.25364331809052	36.0	36.0	36.0	32.0	36.0
42	34.182561220564985	36.0	36.0	36.0	32.0	36.0
43	34.10674601478696	36.0	36.0	36.0	32.0	36.0
44	33.97551177400744	36.0	36.0	36.0	32.0	36.0
45	33.862739860677806	36.0	36.0	36.0	32.0	36.0
46	33.73706141288089	36.0	36.0	36.0	32.0	36.0
47	33.597653745886575	36.0	36.0	36.0	27.0	36.0
48	33.497467840506005	36.0	36.0	36.0	21.0	36.0
49	33.34928843112954	36.0	36.0	36.0	21.0	36.0
50	32.98658062310355	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	6.0
21	6.0
22	19.0
23	52.0
24	87.0
25	185.0
26	400.0
27	823.0
28	1356.0
29	2014.0
30	2876.0
31	3855.0
32	5362.0
33	8141.0
34	15301.0
35	53107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.860624198375376	19.709277469003847	12.918982471141513	23.511115861479265
2	15.881190234521075	21.27357230621294	38.15054222981997	24.69469522944602
3	18.664259156374204	27.245822471045773	28.427496901576994	25.662421471003032
4	12.360570964571135	16.22398393093722	33.98649514936536	37.42894995512629
5	13.574298046925081	37.76977648617462	31.046198555493827	17.60972691140647
6	30.329287576392154	36.70883371084234	16.867173810846616	16.094704901918885
7	27.641138510192743	32.467199452968075	19.495491260310267	20.396170776528912
8	28.396512671481688	31.25560921406898	19.185648959357238	21.162229155092096
9	28.75336552844139	13.029402965938717	19.26364374545921	38.95358776016069
10	17.56378477712723	26.648574725415614	28.128338817898204	27.659301679558958
11	35.20342749690158	23.22214624556605	21.146202829180734	20.42822342835164
12	24.823710414974997	24.73396298987136	27.86764391640668	22.574682678746953
13	29.359160647890935	21.261592375742552	26.598358904226675	22.780888072139835
14	24.058720458139238	18.721953929655115	27.208427710585926	30.010897901619728
15	26.325911363733496	25.461558186247274	24.671994529680756	23.540535920338478
16	23.06081456472499	27.22765930167956	24.334373263814694	25.377152869780762
17	21.133381768451642	25.45728449933758	26.649643147143042	26.759690585067737
18	21.657139804476735	24.897697526577275	29.553929162882632	23.891233506063358
19	24.601478695670757	23.01380400871832	29.835676738322153	22.549040557288773
20	25.81734262147955	21.69857686225907	28.752297106713964	23.731783409547415
21	25.73400572674046	22.05008761058165	29.25338689687593	22.962519765801957
22	24.58972605666909	22.918714474977563	29.14654472413351	23.34501474421984
23	23.241377836659684	23.374930552587717	30.11667165263473	23.267019958117867
24	23.61715085529901	24.079792292158604	29.181669569305395	23.121387283236995
25	23.59929911534681	24.081157314415147	29.11662891576563	23.202914654472416
26	23.04610289011165	23.70746300550243	29.106255676051074	24.140178428334846
27	22.63368769699236	24.575030717452854	29.699236070302902	23.092045515251883
28	22.22364681496677	24.26330747697502	29.804260956898947	23.70878475115926
29	22.357793068079832	24.006367793495446	29.970297875977607	23.665541262447114
30	22.51936535071318	24.334633260323734	29.353063732036965	23.79293765692612
31	22.95076712680029	24.638873456130604	28.613402282148808	23.796957134920298
32	21.83319799991453	24.46472071456045	29.57391341510321	24.128167870421812
33	23.12171460318817	24.449762810376512	29.404034360442754	23.024488225992563
34	22.810803880507713	24.101457327236204	29.489508098636698	23.598230693619385
35	22.257599230728136	24.723542924301512	29.001549228057055	24.017308616913297
36	22.969998717893926	25.227573827941363	28.77900764989957	23.02341980426514
37	23.161246207102867	25.21475276721227	28.38689687593487	23.23710414974999
38	23.1494935681012	25.51925295952818	28.692465489978204	22.63878798239241
39	23.20505149792726	24.837599897431513	28.628360186332753	23.328988418308473
40	22.45822471045771	25.589768793538187	28.50335484422411	23.44865165177999
41	22.78113147069822	25.89668251509162	28.328436348095522	22.993749666114642
42	22.86462228870606	26.544502617801047	27.886526338284007	22.70434875520889
43	22.51901871954868	26.316351824942302	27.624155910761605	23.540473544747414
44	22.168203528192414	26.299030869012384	27.94666039812371	23.58610520467149
45	23.49352536433181	26.09620069233728	26.494721996666527	23.915551946664387
46	23.20932518483696	26.800290610709858	26.34621137655455	23.644172827898625
47	23.116619477536194	26.899941236177145	26.707623270473853	23.27581601581281
48	23.223635003739716	27.73373223635004	25.448231648680412	23.594401111229832
49	23.582960264122317	27.54052119282425	25.526481681322323	23.350036861731112
50	22.940573113661134	27.746436737397694	25.250550248947583	24.06243989999359
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	893.0
1	478.0
2	63.0
3	181.5
4	300.0
5	272.0
6	244.0
7	159.5
8	75.0
9	140.5
10	206.0
11	318.5
12	431.0
13	813.0
14	1195.0
15	1418.5
16	1642.0
17	1600.5
18	1559.0
19	1464.0
20	1369.0
21	1337.0
22	1305.0
23	1095.5
24	886.0
25	685.0
26	484.0
27	498.0
28	512.0
29	568.0
30	624.0
31	842.0
32	1060.0
33	1349.5
34	1639.0
35	1751.5
36	1864.0
37	1863.0
38	1862.0
39	2237.0
40	2612.0
41	3157.5
42	3703.0
43	4011.5
44	4320.0
45	4809.0
46	5298.0
47	5840.5
48	6383.0
49	6973.5
50	7564.0
51	7683.0
52	7802.0
53	7632.0
54	7462.0
55	7530.0
56	7598.0
57	6839.0
58	6080.0
59	5513.0
60	4946.0
61	4387.5
62	3829.0
63	3098.5
64	2368.0
65	2089.0
66	1810.0
67	1514.5
68	1219.0
69	1023.0
70	827.0
71	730.0
72	633.0
73	515.0
74	397.0
75	338.0
76	279.0
77	211.0
78	143.0
79	103.0
80	63.0
81	42.0
82	21.0
83	14.5
84	8.0
85	5.5
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03846318218727296
2	0.0010684217274242488
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0010684217274242488
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0032052651822727465
25	0.0
26	0.0010684217274242488
27	0.0010684217274242488
28	0.0021368434548484977
29	0.0
30	0.0010684217274242488
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0010684217274242488
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0010684217274242488
42	0.006410530364545493
43	0.004273686909696995
44	0.007478952091969742
45	0.0
46	0.0
47	0.0010684217274242488
48	0.006410530364545493
49	0.0032052651822727465
50	0.0021368434548484977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	93596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.46899440147015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.79335219541694	33.53668960211975
2	16.12492170795447	15.953673233898884
3	6.619727435692534	9.82413778366597
4	3.3001447052979413	6.530193598017009
5	1.879009092676184	4.647634514295483
6	1.0842098442798211	3.2180862430018378
7	0.7192069285760567	2.490491046625924
8	0.44491479665665973	1.7607590067951624
9	0.34988445173970323	1.5577588785845549
>10	1.5593615688646034	13.539040129920082
>50	0.0907107837843675	2.879396555408351
>100	0.030236927928122503	2.78858070857729
>500	0.004319561132588929	1.2735586990897048
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	614	0.6560109406384889	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	578	0.6175477584512159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.3985213043292448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.37287918287106286	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.3109107226804564	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	197	0.21047908030257706	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.20513697166545578	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.20193170648318304	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	168	0.17949485020727382	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.1752211632975768	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	125	0.1335527159280311	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	124	0.13248429420060687	No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA	118	0.12607376383606136	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.11432112483439465	No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG	107	0.11432112483439465	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	106	0.11325270310697039	No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT	96	0.10256848583272789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0010684217274242488	0.0
22	0.0	0.0	0.0	0.0010684217274242488	0.0
23	0.0	0.0	0.0	0.005342108637121245	0.0
24	0.0	0.0	0.0	0.00854737381939399	0.0
25	0.0	0.0	0.0	0.00961579554681824	0.0
26	0.0	0.0	0.0	0.01068421727424249	0.0
27	0.0	0.0	0.0	0.012821060729090986	0.0
28	0.0	0.0	0.0	0.01709474763878798	0.0
29	0.0	0.0	0.0	0.02671054318560622	0.0
30	0.0	0.0	0.0	0.05555792982606094	0.0
31	0.0	0.0	0.0	0.08547373819393991	0.0
32	0.0	0.0	0.0	0.11538954656181888	0.0
33	0.0	0.0	0.0	0.13462113765545536	0.0
34	0.0	0.0	0.0	0.1591948373862131	0.0
35	0.0	0.0	0.0	0.18804222402666781	0.0
36	0.0	0.0	0.0	0.22543698448651653	0.0
37	0.0	0.0	0.0	0.2735159622206077	0.0
38	0.0	0.0	0.0	0.32693704859182016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACATG	45	2.3121174E-8	39.11016	1
GTCTAGA	30	1.2921623E-4	36.665775	39
TGTCTAG	30	1.2921623E-4	36.665775	38
ACAGTGC	25	0.0023420283	35.199146	8
TTATAGC	25	0.0023420283	35.199146	4
TAGATGA	35	3.1958497E-4	31.427807	42
ACATGGT	30	0.0057189865	29.33262	10
TGGGTGG	55	4.898073E-6	27.99932	6
TCTAGAT	40	6.984414E-4	27.499332	40
GGCATAG	40	6.984414E-4	27.499332	6
ATAGTTC	40	6.984414E-4	27.499332	9
CTGTCTA	45	0.0013888024	24.44385	37
CATAGTT	45	0.0013888024	24.44385	8
AGGCATA	45	0.0013888024	24.44385	5
CTTCTAG	45	0.0013888024	24.44385	1
GCATAGT	45	0.0013888024	24.44385	7
TTCAGGC	55	1.5786094E-4	23.999416	2
ATATCAA	65	1.7906432E-5	23.691732	1
GAGTGAT	50	0.0025632093	21.999466	7
ACTTCCA	50	0.0025632093	21.999466	16
>>END_MODULE