##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24889
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245409618707058	32.0	32.0	32.0	32.0	32.0
2	31.322391417895457	32.0	32.0	32.0	32.0	32.0
3	31.475953232351642	32.0	32.0	32.0	32.0	32.0
4	31.60070713970027	32.0	32.0	32.0	32.0	32.0
5	31.560368034071278	32.0	32.0	32.0	32.0	32.0
6	35.21559725179798	36.0	36.0	36.0	36.0	36.0
7	35.21254369400137	36.0	36.0	36.0	36.0	36.0
8	35.17425368636747	36.0	36.0	36.0	36.0	36.0
9	35.28281570171562	36.0	36.0	36.0	36.0	36.0
10	35.10470488971031	36.0	36.0	36.0	36.0	36.0
11	35.24818192775925	36.0	36.0	36.0	36.0	36.0
12	35.19177146530596	36.0	36.0	36.0	36.0	36.0
13	35.24070874683595	36.0	36.0	36.0	36.0	36.0
14	35.203985696492424	36.0	36.0	36.0	36.0	36.0
15	35.17947687733537	36.0	36.0	36.0	36.0	36.0
16	35.18590542006509	36.0	36.0	36.0	36.0	36.0
17	35.19116878942505	36.0	36.0	36.0	36.0	36.0
18	35.17031620394552	36.0	36.0	36.0	36.0	36.0
19	35.18184740246695	36.0	36.0	36.0	36.0	36.0
20	35.18703041504279	36.0	36.0	36.0	36.0	36.0
21	35.15227610591024	36.0	36.0	36.0	36.0	36.0
22	35.120414641006064	36.0	36.0	36.0	36.0	36.0
23	35.08967817107959	36.0	36.0	36.0	36.0	36.0
24	35.079513037888226	36.0	36.0	36.0	36.0	36.0
25	35.079633573064406	36.0	36.0	36.0	36.0	36.0
26	34.996464301498655	36.0	36.0	36.0	36.0	36.0
27	35.02912933424405	36.0	36.0	36.0	36.0	36.0
28	34.938125276226444	36.0	36.0	36.0	36.0	36.0
29	34.947446663184536	36.0	36.0	36.0	36.0	36.0
30	34.9205673188959	36.0	36.0	36.0	32.0	36.0
31	34.92293784402748	36.0	36.0	36.0	36.0	36.0
32	34.863473823777575	36.0	36.0	36.0	32.0	36.0
33	34.85037566796577	36.0	36.0	36.0	32.0	36.0
34	34.86106312025393	36.0	36.0	36.0	32.0	36.0
35	34.805014263329184	36.0	36.0	36.0	32.0	36.0
36	34.77753224315963	36.0	36.0	36.0	32.0	36.0
37	34.816545461850616	36.0	36.0	36.0	32.0	36.0
38	34.74004580336695	36.0	36.0	36.0	32.0	36.0
39	34.711720036964124	36.0	36.0	36.0	32.0	36.0
40	34.71208164249267	36.0	36.0	36.0	32.0	36.0
41	34.66330507453092	36.0	36.0	36.0	32.0	36.0
42	34.732411908875406	36.0	36.0	36.0	32.0	36.0
43	34.64835871268432	36.0	36.0	36.0	32.0	36.0
44	34.628711478966615	36.0	36.0	36.0	32.0	36.0
45	34.590381292940656	36.0	36.0	36.0	32.0	36.0
46	34.54939933303869	36.0	36.0	36.0	32.0	36.0
47	34.60769817991884	36.0	36.0	36.0	32.0	36.0
48	34.5518502149544	36.0	36.0	36.0	32.0	36.0
49	34.51295753143959	36.0	36.0	36.0	32.0	36.0
50	34.05468279159468	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	2.0
23	2.0
24	7.0
25	23.0
26	57.0
27	94.0
28	174.0
29	310.0
30	441.0
31	717.0
32	1146.0
33	2151.0
34	4673.0
35	15089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.264418632691616	20.742735420602067	12.005144487761745	25.987701458944578
2	15.299931696733497	22.40347141307405	37.50652898870987	24.790067901482583
3	17.473582707220057	24.938727952107357	31.001647314074493	26.586042026598093
4	12.097713849491743	18.77134477078227	39.16589658081884	29.965044798907147
5	13.471814857969383	36.25296315641448	35.802965165334086	14.472256820282054
6	30.04138374382257	37.904295070111296	19.028486479971072	13.025834706095063
7	27.55032343605609	31.371288521033385	23.283378199204467	17.795009843706055
8	24.179356342159185	36.429748081481776	21.748563622483825	17.642331953875207
9	24.854353328779784	16.87090682630881	21.559725179798306	36.7150146651131
10	15.332074410382097	28.261480975531363	34.88689782634899	21.519546787737553
11	33.04270963076058	22.84945156494837	25.408815139218127	18.69902366507292
12	21.929366386757202	25.58158222507935	31.793161637671258	20.695889750492185
13	27.86773273333601	21.575796536622605	26.642291775483145	23.91417895455824
14	21.222226686488007	22.238740005624972	27.98425007031219	28.55478323757483
15	22.974004580336697	29.985133994937524	23.91417895455824	23.126682470167545
16	22.672666639881072	28.928442283739802	26.115954839487326	22.2829362368918
17	21.33874402346418	28.61906866487203	28.22130258347061	21.820884728193178
18	21.953473421993653	27.91594680380891	28.526658363132306	21.60392141106513
19	22.676684479087147	27.77934027080236	28.040499819197233	21.503475430913255
20	22.652577443850696	28.462372935835106	27.642733737795815	21.24231588251838
21	22.805255333681547	27.60657318494114	26.879344288641573	22.708827192735747
22	22.21061513118245	28.309695046004258	27.895857607778535	21.583832215034754
23	22.102133472618426	28.14898147776126	27.27711037004299	22.471774679577322
24	22.471774679577322	27.795411627626663	27.99630358793041	21.7365101048656
25	22.3793643778376	27.60657318494114	27.84362569809956	22.1704367391217
26	21.828920406605327	27.815500823657036	27.803447306038816	22.552131463698824
27	22.4757925187834	27.723090521917314	27.393627707019164	22.407489252280126
28	22.284726965885802	28.44858761602443	27.22304817776349	22.043637240326277
29	22.286954076097874	27.863714894129938	27.377556350194865	22.471774679577322
30	21.989633974848328	27.891839768572463	27.827554341275263	22.29097191530395
31	22.773112620032947	27.152557354654665	27.799429466832738	22.27490055847965
32	21.704367391217	28.892281730885127	27.53425207923179	21.86909879866608
33	21.905259351520755	27.26907469163084	28.41415886536221	22.4115070914862
34	21.94945558278758	27.602555345735063	28.79183575073326	21.656153320744103
35	22.00168749246655	28.377998312507536	27.731126200329463	21.889187994696453
36	22.21061513118245	28.13291012093696	28.080678211257986	21.575796536622605
37	22.13025834706095	28.128892281730884	27.373538510988794	22.367310860219376
38	21.861063120253927	27.964160874281813	28.177106352203786	21.997669653260477
39	22.495881714813773	27.78335811000844	27.799429466832738	21.921330708345053
40	22.79320181606332	27.662822933826188	27.731126200329463	21.81284904978103
41	22.676684479087147	27.46996665193459	27.654787255414035	22.198561613564223
42	21.591867893446903	27.952107356663586	28.209249065852383	22.246775684037125
43	22.198561613564223	27.972196552693962	27.77934027080236	22.04990156293945
44	21.757473481195756	28.134040501446478	27.925104468016716	22.183381549341046
45	22.347221664189	28.29362368917996	27.248985495600465	22.110169151030576
46	22.254811362449274	28.281570171561732	27.461930973522442	22.00168749246655
47	22.640523926232472	27.827554341275263	27.823536502069185	21.708385230423076
48	21.463297038852506	28.390051830125756	28.06058901522761	22.086062115794125
49	22.123111539697845	27.157666345226616	27.985374477659917	22.73384763741562
50	22.1061513118245	28.012374944754708	27.586483988910764	22.294989754510024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	3.0
7	1.5
8	0.0
9	3.5
10	7.0
11	8.0
12	9.0
13	10.0
14	11.0
15	14.5
16	18.0
17	32.5
18	47.0
19	67.0
20	87.0
21	102.5
22	118.0
23	182.5
24	247.0
25	293.0
26	339.0
27	454.5
28	570.0
29	713.0
30	856.0
31	910.0
32	964.0
33	1137.5
34	1311.0
35	1471.0
36	1631.0
37	1660.5
38	1690.0
39	1713.5
40	1737.0
41	1746.5
42	1756.0
43	1740.0
44	1724.0
45	1682.5
46	1641.0
47	1629.0
48	1617.0
49	1538.0
50	1459.0
51	1398.5
52	1338.0
53	1302.0
54	1266.0
55	1166.5
56	1067.0
57	949.0
58	831.0
59	762.5
60	694.0
61	618.0
62	542.0
63	469.5
64	397.0
65	336.5
66	276.0
67	242.0
68	208.0
69	182.0
70	156.0
71	123.5
72	91.0
73	79.5
74	68.0
75	56.0
76	44.0
77	38.0
78	32.0
79	23.0
80	14.0
81	12.5
82	11.0
83	8.5
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03214271364859979
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.008035678412149947
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0040178392060749735
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0040178392060749735
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	24889.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.68395676805015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.82853711884033	87.90228615050826
2	5.699704078569284	10.679416609747278
3	0.4245829223313462	1.193298244204267
4	0.03002101471029721	0.11249949777009925
5	0.004288716387185316	0.020089196030374866
6	0.004288716387185316	0.024107035236449838
7	0.0	0.0
8	0.004288716387185316	0.03214271364859979
9	0.004288716387185316	0.03616055285467476
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.004017839206074973	0.0
32	0.0	0.0	0.0	0.004017839206074973	0.0
33	0.0	0.0	0.0	0.008035678412149945	0.0
34	0.0	0.0	0.0	0.024107035236449838	0.0
35	0.0	0.0	0.0	0.024107035236449838	0.0
36	0.0	0.0	0.0	0.04017839206074973	0.0
37	0.0	0.0	0.0	0.04017839206074973	0.0
38	0.0	0.0	0.0	0.0723211057093495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAATGC	20	7.7085546E-4	44.0	8
>>END_MODULE