FastQCFastQC Report
Thu 2 Feb 2017
SRR4061963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586917
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39220.15160903886750135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.013.3250521
GCGTTAT1352.7284841E-1112.6621141
GTATCAA24900.012.28072551
CGATTAG550.003070094212.0905418
CGTTATT1501.7826096E-1011.3965642
GTCCTAA3500.010.8532411
GATATAC2053.6379788E-1210.1914591
TTACGCT750.002649258810.1330244
GTATAGG2350.010.1027511
GTTATTC2107.2759576E-129.9511143
GGGTAGG5750.09.9094811
TAGGACT3300.09.787584
CTAGAAC1752.581146E-99.77018453
CAGTGCG2251.8189894E-129.7123179
TGATCGA1002.7524523E-49.50136410
AACCGGG800.0045139729.5008137
CGCATCG1701.6636477E-89.50081313
CGTCTTA900.00111375879.50026115
CGAGCCG3300.09.50026115
TCTATAC1606.652772E-89.4987913
Produced by FastQC (version 0.11.5)