##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061963_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2586917
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248005251038204	32.0	32.0	32.0	32.0	32.0
2	30.715824280407915	32.0	32.0	32.0	32.0	32.0
3	30.74595667352296	32.0	32.0	32.0	32.0	32.0
4	30.76103717282	32.0	32.0	32.0	32.0	32.0
5	30.641993925587872	32.0	32.0	32.0	32.0	32.0
6	34.328472850114636	36.0	36.0	36.0	32.0	36.0
7	34.2634089149362	36.0	36.0	36.0	32.0	36.0
8	34.20934301332436	36.0	36.0	36.0	32.0	36.0
9	34.367698693077514	36.0	36.0	36.0	32.0	36.0
10	34.0005435041016	36.0	36.0	36.0	32.0	36.0
11	34.36048508707469	36.0	36.0	36.0	32.0	36.0
12	34.15295504262409	36.0	36.0	36.0	32.0	36.0
13	34.247043875006426	36.0	36.0	36.0	32.0	36.0
14	34.14360916875184	36.0	36.0	36.0	32.0	36.0
15	34.08083599125909	36.0	36.0	36.0	32.0	36.0
16	34.088814600545746	36.0	36.0	36.0	32.0	36.0
17	34.03021975579425	36.0	36.0	36.0	32.0	36.0
18	34.02618251764552	36.0	36.0	36.0	32.0	36.0
19	34.03232419130571	36.0	36.0	36.0	32.0	36.0
20	34.01940649815978	36.0	36.0	36.0	32.0	36.0
21	34.00952059923067	36.0	36.0	36.0	32.0	36.0
22	33.97478504335469	36.0	36.0	36.0	32.0	36.0
23	33.9322572003663	36.0	36.0	36.0	32.0	36.0
24	33.903270572654634	36.0	36.0	36.0	32.0	36.0
25	33.536895076262596	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	41.0
5	197.0
6	510.0
7	108.0
8	447.0
9	382.0
10	158.0
11	51.0
12	107.0
13	107.0
14	397.0
15	579.0
16	936.0
17	1236.0
18	1730.0
19	2411.0
20	3638.0
21	5314.0
22	8094.0
23	11715.0
24	16976.0
25	23728.0
26	32248.0
27	41632.0
28	53836.0
29	71200.0
30	92966.0
31	125747.0
32	177442.0
33	249392.0
34	535036.0
35	1128555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49757701894471	16.858582641126663	11.806234189546082	25.83760615038254
2	16.678373026073935	19.016140086659274	37.532495294584706	26.77299159268209
3	19.574099945185313	22.499302253123236	27.954368513949913	29.972229287741538
4	13.07897472535077	15.2932175548187	34.90721443032974	36.7205932895008
5	15.103928008108058	36.119415220552895	33.37304611748565	15.403610653853397
6	35.32753118653902	34.34530509621894	16.372691229088097	13.954472488153948
7	31.073739070942036	29.9589500055337	19.665067956160712	19.30224296736355
8	28.257954614494864	33.43242539362393	18.60305735510882	19.70656263677239
9	27.00491847118327	14.830841202151912	18.178412520758087	39.98582780590673
10	16.088330273567454	26.9939474308611	31.417970212085706	25.49975208348574
11	36.93366654124919	21.41936972298221	21.951968202798515	19.694995532970086
12	24.66532047835745	23.302062903919392	28.619313196245074	23.41330342147809
13	29.595076827805084	19.680380849169797	24.835391105778534	25.889151217246585
14	23.41543627070053	19.33361248796436	25.028986157851886	32.22196508348323
15	25.1079042291181	27.146075870288104	21.99427065411387	25.75174924647993
16	25.735363552346612	26.076979997616	23.328993626668606	24.85866282336878
17	24.218374153216896	26.138258785973406	24.819438730310104	24.823928330499594
18	24.663157735868918	25.675087352408386	25.494210109389325	24.16754480233337
19	25.642125904733753	25.14105450186538	25.049855637266095	24.166963956134776
20	25.66452008301568	24.932793514748283	24.83954790193502	24.56313850030102
21	25.953784689147014	24.619998932135676	24.66975521918689	24.756461159530417
22	25.703654604347975	24.545907274189936	24.848693800208874	24.901744321253215
23	24.706580534542216	24.694703709979553	25.143547092963423	25.45516866251481
24	24.80700956130658	24.979958089967283	25.094018998802127	25.119013349924014
25	25.010978177897165	24.68730086939431	25.16972166304983	25.13199928965869
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	462.0
3	709.0
4	709.0
5	709.0
6	1505.0
7	2301.0
8	2301.0
9	2301.0
10	2882.5
11	3464.0
12	3464.0
13	3464.0
14	4925.5
15	6387.0
16	6387.0
17	6387.0
18	10805.5
19	15224.0
20	15224.0
21	15224.0
22	24034.0
23	32844.0
24	32844.0
25	32844.0
26	48513.5
27	64183.0
28	64183.0
29	64183.0
30	83838.5
31	103494.0
32	103494.0
33	103494.0
34	127995.0
35	152496.0
36	152496.0
37	152496.0
38	181089.0
39	209682.0
40	209682.0
41	209682.0
42	241098.0
43	272514.0
44	272514.0
45	272514.0
46	302009.5
47	331505.0
48	331505.0
49	331505.0
50	344787.5
51	358070.0
52	358070.0
53	358070.0
54	342154.0
55	326238.0
56	326238.0
57	326238.0
58	297984.0
59	269730.0
60	269730.0
61	269730.0
62	234595.0
63	199460.0
64	199460.0
65	199460.0
66	160946.0
67	122432.0
68	122432.0
69	122432.0
70	92436.0
71	62440.0
72	62440.0
73	62440.0
74	45682.0
75	28924.0
76	28924.0
77	28924.0
78	21712.0
79	14500.0
80	14500.0
81	14500.0
82	10014.0
83	5528.0
84	5528.0
85	5528.0
86	3920.0
87	2312.0
88	2312.0
89	2312.0
90	1629.0
91	946.0
92	946.0
93	946.0
94	601.0
95	256.0
96	256.0
97	256.0
98	659.5
99	1063.0
100	1063.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.117771076536278E-4
2	1.5462421098164339E-4
3	7.344650021628062E-4
4	0.009818637397334356
5	0.0327803327281084
6	0.06443963992659989
7	0.10634280110262526
8	0.10777307505420546
9	0.11534966139230597
10	0.13088939459596113
11	0.13703570698248146
12	0.1285700314312365
13	0.1243565216819867
14	0.11306895428032675
15	0.13050283406850705
16	0.11693455955486783
17	0.12246237509746158
18	0.10993781400794846
19	0.09490060948998363
20	0.09064844368798844
21	0.08964338631660776
22	0.10004186450512328
23	0.07928356418083765
24	0.0901845710550435
25	0.08674418236070194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2586917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.787416222352064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89296672186626	46.96701087916874
2	11.69521365374587	13.750627857441867
3	3.427130425755387	6.044164283615057
4	1.5306641214997794	3.5993515522891353
5	0.865738969322928	2.544727856474853
6	0.5463877198914648	1.9272433384824872
7	0.35708167864886237	1.4694336487674786
8	0.2708122816626921	1.2736283456183617
9	0.20074721904510756	1.0621269289335977
>10	1.0918924534559507	11.610639834505784
>50	0.07393006293253815	2.9681509901978487
>100	0.04353344167351428	4.853874538406826
>500	0.0032400216014977818	1.3113059720257316
>1k	6.61228898260201E-4	0.617713974072146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3922	0.15160903886750135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.546242109816434E-4
2	0.0	0.0	0.0	7.73121054908217E-5	1.546242109816434E-4
3	0.0	0.0	0.0	7.73121054908217E-5	1.546242109816434E-4
4	0.0	0.0	0.0	1.1596815823623255E-4	1.546242109816434E-4
5	0.0	0.0	0.0	1.1596815823623255E-4	1.546242109816434E-4
6	0.0	0.0	0.0	1.1596815823623255E-4	2.319363164724651E-4
7	0.0	0.0	0.0	1.1596815823623255E-4	2.319363164724651E-4
8	0.0	0.0	0.0	1.1596815823623255E-4	2.319363164724651E-4
9	0.0	0.0	0.0	1.1596815823623255E-4	2.7059236921787595E-4
10	0.0	0.0	0.0	1.1596815823623255E-4	2.7059236921787595E-4
11	0.0	3.865605274541085E-5	0.0	1.1596815823623255E-4	2.7059236921787595E-4
12	0.0	3.865605274541085E-5	0.0	1.1596815823623255E-4	6.958089494173953E-4
13	0.0	3.865605274541085E-5	0.0	1.1596815823623255E-4	7.73121054908217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	955	0.0	13.325052	1
GCGTTAT	135	2.7284841E-11	12.662114	1
GTATCAA	2490	0.0	12.2807255	1
CGATTAG	55	0.0030700942	12.09054	18
CGTTATT	150	1.7826096E-10	11.396564	2
GTCCTAA	350	0.0	10.853241	1
GATATAC	205	3.6379788E-12	10.191459	1
TTACGCT	75	0.0026492588	10.133024	4
GTATAGG	235	0.0	10.102751	1
GTTATTC	210	7.2759576E-12	9.951114	3
GGGTAGG	575	0.0	9.909481	1
TAGGACT	330	0.0	9.78758	4
CTAGAAC	175	2.581146E-9	9.7701845	3
CAGTGCG	225	1.8189894E-12	9.712317	9
TGATCGA	100	2.7524523E-4	9.501364	10
AACCGGG	80	0.004513972	9.500813	7
CGCATCG	170	1.6636477E-8	9.500813	13
CGTCTTA	90	0.0011137587	9.500261	15
CGAGCCG	330	0.0	9.500261	15
TCTATAC	160	6.652772E-8	9.498791	3
>>END_MODULE