FastQCFastQC Report
Thu 2 Feb 2017
SRR4061963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586917
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29050.029.2404231
GGTATCA11400.024.9016061
TCAACGC40750.020.2983174
ATCAACG41000.020.22823
CAACGCA42550.019.5430365
TATCAAC42900.019.4872822
AACGCAG43400.019.160286
GTATAGG3400.016.828171
ATACCGT4500.016.1324846
ACGCAGA51700.015.9991567
GTCCTAC6150.015.7441821
TAGACAG5750.015.6861295
AGAGTAC53400.015.53101511
GCATATA2700.015.4857531
CGCAGAG53950.015.3319078
GGCGTTA4200.015.19202442
CAGAGTA56100.014.82274810
CCTATAC3450.014.6658943
TAGGACC8900.014.58354
TAGGACA5400.014.2585074
Produced by FastQC (version 0.11.5)