##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2586917
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290755366329883	32.0	32.0	32.0	32.0	32.0
2	31.460968403702168	32.0	32.0	32.0	32.0	32.0
3	31.585288588694574	32.0	32.0	32.0	32.0	32.0
4	31.662853118209824	32.0	32.0	32.0	32.0	32.0
5	31.643204633159858	32.0	32.0	32.0	32.0	32.0
6	35.29569251738653	36.0	36.0	36.0	36.0	36.0
7	35.30385976821058	36.0	36.0	36.0	36.0	36.0
8	35.260287052116475	36.0	36.0	36.0	36.0	36.0
9	35.334098465470674	36.0	36.0	36.0	36.0	36.0
10	35.228106274766446	36.0	36.0	36.0	36.0	36.0
11	35.33909901245382	36.0	36.0	36.0	36.0	36.0
12	35.272093770306505	36.0	36.0	36.0	36.0	36.0
13	35.30421153829056	36.0	36.0	36.0	36.0	36.0
14	35.266039072765	36.0	36.0	36.0	36.0	36.0
15	35.243942499894665	36.0	36.0	36.0	36.0	36.0
16	35.26086650634713	36.0	36.0	36.0	36.0	36.0
17	35.24439206978809	36.0	36.0	36.0	36.0	36.0
18	35.23747186322561	36.0	36.0	36.0	36.0	36.0
19	35.22727091746662	36.0	36.0	36.0	36.0	36.0
20	35.22836217783563	36.0	36.0	36.0	36.0	36.0
21	35.22546297387972	36.0	36.0	36.0	36.0	36.0
22	35.20596833991968	36.0	36.0	36.0	36.0	36.0
23	35.17944951461527	36.0	36.0	36.0	36.0	36.0
24	35.1630450455117	36.0	36.0	36.0	36.0	36.0
25	35.14745621912106	36.0	36.0	36.0	36.0	36.0
26	35.106226059823335	36.0	36.0	36.0	36.0	36.0
27	35.090121175128544	36.0	36.0	36.0	36.0	36.0
28	35.07698739464776	36.0	36.0	36.0	36.0	36.0
29	35.05885809247069	36.0	36.0	36.0	36.0	36.0
30	35.04210765169505	36.0	36.0	36.0	36.0	36.0
31	35.04020461421839	36.0	36.0	36.0	36.0	36.0
32	35.01319563016517	36.0	36.0	36.0	36.0	36.0
33	35.00235183424903	36.0	36.0	36.0	36.0	36.0
34	34.991938280199946	36.0	36.0	36.0	36.0	36.0
35	34.978544731044714	36.0	36.0	36.0	36.0	36.0
36	34.96025539280928	36.0	36.0	36.0	36.0	36.0
37	34.945978552848814	36.0	36.0	36.0	36.0	36.0
38	34.92743872339159	36.0	36.0	36.0	36.0	36.0
39	34.908473677354166	36.0	36.0	36.0	36.0	36.0
40	34.90193423291122	36.0	36.0	36.0	36.0	36.0
41	34.87941630906597	36.0	36.0	36.0	36.0	36.0
42	34.85599885887332	36.0	36.0	36.0	32.0	36.0
43	34.84631087893427	36.0	36.0	36.0	32.0	36.0
44	34.810875261943075	36.0	36.0	36.0	32.0	36.0
45	34.797949451026064	36.0	36.0	36.0	32.0	36.0
46	34.78568079300573	36.0	36.0	36.0	32.0	36.0
47	34.75444129054005	36.0	36.0	36.0	32.0	36.0
48	34.73556515342394	36.0	36.0	36.0	32.0	36.0
49	34.72138070142954	36.0	36.0	36.0	32.0	36.0
50	34.31454391462888	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	12.0
21	51.0
22	146.0
23	429.0
24	1067.0
25	2596.0
26	5467.0
27	10399.0
28	18093.0
29	29323.0
30	44270.0
31	66046.0
32	101221.0
33	174351.0
34	407320.0
35	1726116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.173364293544566	17.34516833164805	12.197910765762543	26.28355660904484
2	16.115814939179256	19.399199468683484	37.28938116934477	27.195604422792492
3	18.935300777878155	22.879158099492482	28.2420835058365	29.943457616792863
4	12.891175093750592	15.67595713352999	34.98596978565605	36.446897987063366
5	14.81787007468736	36.418679068559214	33.220161296245685	15.54328956050774
6	35.52418308940663	34.47652431799935	16.243427006186142	13.75586558640788
7	31.187896635261204	29.980977356443983	19.70492288697318	19.126203121321637
8	28.352011293752373	33.48163083701564	18.524676284550296	19.641681584681688
9	27.130016154364444	14.611794657501576	18.05423212263865	40.20395706549534
10	15.997072963686119	27.082585177645825	31.488254165093043	25.432087693575017
11	37.272204713177885	21.20968705219379	21.8998908739631	19.618217360665223
12	24.691515035078435	23.20921003650291	28.674673366018315	23.42460156240034
13	29.692757827174198	19.57654613580567	24.87223208166323	25.858463955356896
14	23.430322658206663	19.189560391771362	25.1045936147159	32.27552333530608
15	25.171893802545657	27.0941433374167	21.974303775498015	25.759659084539628
16	25.76582768534006	25.986168037737734	23.385610692614772	24.862393584307437
17	24.253271365103714	26.10458704318693	24.849966195281876	24.792175396427485
18	24.735234621342116	25.49803568964244	25.562436773095964	24.204292915919474
19	25.616245128854153	25.08600005334535	25.158712088559472	24.139042729241023
20	25.773693624321538	24.72471973308686	24.965238437069104	24.536348205522497
21	26.014054567657176	24.471794031273518	24.76314470081568	24.751006700253622
22	25.681999074574097	24.468585578895652	24.977299233025256	24.872116113504994
23	24.770798599259273	24.57616537368613	25.210743135554793	25.442292891499807
24	24.912114546811033	24.788955077332243	25.192489823687904	25.10644055216882
25	25.09940415491199	24.54882535716301	25.28758203790308	25.064188450021916
26	24.803674813203568	25.10012922768387	25.41475205718644	24.681443901926123
27	25.107473906097127	25.099742665662124	24.86003555597476	24.932747872265992
28	25.000773132112812	24.78147420831272	25.1453488372093	25.072403822365164
29	24.87900613396379	25.031543448781324	25.096795092828966	24.992655324425918
30	24.97993728371696	24.969036154638314	25.191079010765062	24.859947550879667
31	25.22781066346799	24.74205682693657	24.882688090449328	25.147444419146108
32	25.104227107436355	24.92548027580512	24.75593364874315	25.21435896801537
33	24.777756345110948	24.766855321381122	25.087817023610764	25.367571309897162
34	25.21124697079633	24.810419880776266	25.217393321197832	24.76093982722957
35	25.34897771977643	24.750774378574	25.27402351760573	24.62622438404384
36	24.8146729325648	25.008185431825503	25.073707542542017	25.103434093067683
37	25.5057428075742	24.76005919081151	24.814642149176926	24.91955585243737
38	25.022797407106605	24.836823137348436	25.072663715148185	25.067715740396775
39	25.321697784993624	24.793807259654415	24.779890989214888	25.104603966137073
40	25.538363054695246	24.796359835417924	24.950558889426638	24.714718220460195
41	24.894603335119257	24.964610069063518	25.2395343605111	24.901252235306124
42	25.539606168871583	24.986295777707852	24.857990238109412	24.616107815311153
43	25.11553827984715	24.58349199108553	25.12721291322693	25.17375681584039
44	24.88160132301896	24.927333307303858	24.98953344484773	25.20153192482945
45	24.98259478929934	25.058400715304874	24.9596713246786	24.999333170717186
46	25.041691181416365	24.86634468266381	24.95336076923612	25.138603366683704
47	25.197756536509242	24.9251515234685	25.043130890995492	24.83396104902677
48	25.317106020027584	25.244236966297002	24.572681952287585	24.86597506138783
49	25.148474203585646	25.08778176079987	24.677431263875665	25.086312771738818
50	25.051229765643875	25.274510751389222	24.74421841024402	24.93004107272288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	50.5
2	67.0
3	178.5
4	290.0
5	303.0
6	316.0
7	269.0
8	222.0
9	304.0
10	386.0
11	631.0
12	876.0
13	1631.5
14	2387.0
15	3199.5
16	4012.0
17	3954.0
18	3896.0
19	3713.0
20	3530.0
21	4457.5
22	5385.0
23	6781.0
24	8177.0
25	10983.0
26	13789.0
27	17760.5
28	21732.0
29	26033.0
30	30334.0
31	35433.0
32	40532.0
33	47298.5
34	54065.0
35	62709.5
36	71354.0
37	81580.0
38	91806.0
39	100869.5
40	109933.0
41	119797.5
42	129662.0
43	136980.0
44	144298.0
45	156369.5
46	168441.0
47	177938.5
48	187436.0
49	193684.5
50	199933.0
51	201758.0
52	203583.0
53	203469.5
54	203356.0
55	199568.5
56	195781.0
57	187345.0
58	178909.0
59	165547.0
60	152185.0
61	133945.5
62	115706.0
63	99792.0
64	83878.0
65	70098.5
66	56319.0
67	47280.0
68	38241.0
69	32388.5
70	26536.0
71	20720.0
72	14904.0
73	12575.5
74	10247.0
75	7854.0
76	5461.0
77	4574.0
78	3687.0
79	3016.5
80	2346.0
81	1871.5
82	1397.0
83	1083.5
84	770.0
85	593.0
86	416.0
87	279.5
88	143.0
89	100.0
90	57.0
91	42.5
92	28.0
93	26.0
94	24.0
95	21.0
96	18.0
97	21.0
98	24.0
99	16.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031002154301819504
2	0.005218567120630465
3	3.0924842196328677E-4
4	0.0
5	0.0
6	2.3193631647246512E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.865605274541085E-4
17	0.0
18	1.5462421098164339E-4
19	0.0
20	3.0924842196328677E-4
21	0.0
22	0.0
23	0.0
24	0.001043713424126093
25	1.1596815823623256E-4
26	3.865605274541085E-4
27	3.865605274541085E-4
28	0.0014302739515802013
29	3.4790447470869765E-4
30	0.0011210255296169147
31	3.865605274541085E-4
32	6.958089494173953E-4
33	1.5462421098164339E-4
34	6.184968439265735E-4
35	1.5462421098164339E-4
36	1.5462421098164339E-4
37	0.0011210255296169147
38	0.0
39	6.571528966719844E-4
40	3.8656052745410846E-5
41	8.890892131444495E-4
42	0.004754694487685535
43	0.004329477907486015
44	0.00409754159101355
45	0.001855490531779721
46	0.0015075860570710232
47	9.277452658898605E-4
48	0.0032857644833599224
49	0.004020229485522728
50	0.0016235542153072557
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2586917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.23481436630546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93567027257868	48.14910260114978
2	11.882600948450307	14.31492524637579
3	3.3833551937030255	6.113873160839316
4	1.4802201779912867	3.5664315057025906
5	0.8216547982226408	2.4746112122062467
6	0.5114987515591253	1.8486019409256542
7	0.36033181159272315	1.519316384709356
8	0.26005957266345026	1.2531712066850922
9	0.19420588166182404	1.0528159707670188
>10	1.064992151003259	11.530016696074847
>50	0.06620156260270205	2.7145997188333952
>100	0.03598618637599307	3.993797003990581
>500	0.002578153276075074	1.0127924531476373
>1k	6.44538319006754E-4	0.45594489859278914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.73121054908217E-5
2	0.0	0.0	0.0	0.0	7.73121054908217E-5
3	0.0	0.0	0.0	3.865605274541085E-5	7.73121054908217E-5
4	0.0	0.0	0.0	7.73121054908217E-5	7.73121054908217E-5
5	0.0	0.0	0.0	7.73121054908217E-5	1.1596815823623255E-4
6	0.0	0.0	0.0	7.73121054908217E-5	3.092484219632868E-4
7	0.0	0.0	0.0	7.73121054908217E-5	3.092484219632868E-4
8	0.0	0.0	0.0	7.73121054908217E-5	3.4790447470869765E-4
9	0.0	0.0	0.0	1.546242109816434E-4	3.4790447470869765E-4
10	0.0	0.0	0.0	1.546242109816434E-4	3.4790447470869765E-4
11	0.0	0.0	0.0	1.546242109816434E-4	3.4790447470869765E-4
12	0.0	0.0	0.0	1.546242109816434E-4	7.344650021628062E-4
13	0.0	0.0	0.0	1.546242109816434E-4	0.0011210255296169147
14	0.0	0.0	0.0	1.546242109816434E-4	0.0011210255296169147
15	0.0	0.0	0.0	3.092484219632868E-4	0.0011983376351077364
16	0.0	0.0	0.0	5.411847384357519E-4	0.001275649740598558
17	0.0	0.0	0.0	6.571528966719844E-4	0.0013143057933439688
18	0.0	0.0	0.0	8.504331603990387E-4	0.001546242109816434
19	0.0	0.0	0.0	8.890892131444496E-4	0.001546242109816434
20	0.0	0.0	0.0	9.664013186352713E-4	0.0018941465845251316
21	0.0	0.0	0.0	0.0010823694768715038	0.0019328026372705425
22	0.0	0.0	0.0	0.0014302739515802015	0.0019328026372705425
23	0.0	0.0	0.0	0.0017781784262888992	0.0019328026372705425
24	0.0	0.0	0.0	0.002667267639433349	0.0019328026372705425
25	0.0	0.0	0.0	0.0032857644833599224	0.0019328026372705425
26	0.0	0.0	0.0	0.0038269492217956743	0.002048770795506775
27	0.0	0.0	0.0	0.004368133960231426	0.002048770795506775
28	0.0	0.0	0.0	0.005257223173375875	0.002087426848252186
29	0.0	0.0	0.0	0.006455560808483612	0.002087426848252186
30	0.0	3.865605274541085E-5	0.0	0.008542987656735798	0.002087426848252186
31	0.0	3.865605274541085E-5	0.0	0.012601873195003938	0.0022420510592338293
32	3.865605274541085E-5	3.865605274541085E-5	0.0	0.01770447215739817	0.0022420510592338293
33	3.865605274541085E-5	3.865605274541085E-5	0.0	0.021299485062721377	0.00228070711197924
34	3.865605274541085E-5	3.865605274541085E-5	0.0	0.026518052183351844	0.002396675270215473
35	3.865605274541085E-5	3.865605274541085E-5	0.0	0.031504682987509844	0.002396675270215473
36	3.865605274541085E-5	3.865605274541085E-5	0.0	0.039313205642082835	0.002396675270215473
37	3.865605274541085E-5	3.865605274541085E-5	0.0	0.05063942909648821	0.002396675270215473
38	3.865605274541085E-5	3.865605274541085E-5	0.0	0.06780271651545063	0.002396675270215473
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2905	0.0	29.240423	1
GGTATCA	1140	0.0	24.901606	1
TCAACGC	4075	0.0	20.298317	4
ATCAACG	4100	0.0	20.2282	3
CAACGCA	4255	0.0	19.543036	5
TATCAAC	4290	0.0	19.487282	2
AACGCAG	4340	0.0	19.16028	6
GTATAGG	340	0.0	16.82817	1
ATACCGT	450	0.0	16.132484	6
ACGCAGA	5170	0.0	15.999156	7
GTCCTAC	615	0.0	15.744182	1
TAGACAG	575	0.0	15.686129	5
AGAGTAC	5340	0.0	15.531015	11
GCATATA	270	0.0	15.485753	1
CGCAGAG	5395	0.0	15.331907	8
GGCGTTA	420	0.0	15.192024	42
CAGAGTA	5610	0.0	14.822748	10
CCTATAC	345	0.0	14.665894	3
TAGGACC	890	0.0	14.5835	4
TAGGACA	540	0.0	14.258507	4
>>END_MODULE