FastQCFastQC Report
Thu 2 Feb 2017
SRR4061962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482755
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84200.24176262757500885No Hit
GTATCAACGCAGAGTACATGGGGGG76260.21896458407209235No Hit
GGTATCAACGCAGAGTACATGGGGG47220.13558231916973776No Hit
TATCAACGCAGAGTACTTTTTTTTT46630.13388825800264445No Hit
GTATCAACGCAGAGTACATGGGGGA35550.10207436354265516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.015.3500651
TCGCGTA400.005273393414.25303211
ACGGGTC400.00527742214.2513925
GTATCAA67400.013.7207471
GTACGCT500.001501859713.2980463
CGTTCAA802.8675742E-511.87547713
GCCGATC658.0134853E-411.6947978
GTAGGCG1302.6266207E-911.69059118
ACCGTGT2100.011.3119318
TATAGTT1354.7293724E-911.2603585
GGTTATA1701.2732926E-1111.170171
GCGTACT600.00587637911.08377813
ACGGCGC700.0014895610.85945310
CTTACAC2900.010.8087583
TATTCCG803.7698008E-410.6885445
CCCGTCG1002.3943254E-510.4525249
TGGACCG2400.010.2926725
CAACGTT1201.528424E-610.29000819
ATACCGA1303.8353392E-710.23286
CTAACAC2600.010.2292663
Produced by FastQC (version 0.11.5)