##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061962_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3482755
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.372503377354995	32.0	32.0	32.0	32.0	32.0
2	31.067967456797852	32.0	32.0	32.0	32.0	32.0
3	31.080393251893973	32.0	32.0	32.0	32.0	32.0
4	31.073387016887494	32.0	32.0	32.0	32.0	32.0
5	31.07001267674585	32.0	32.0	32.0	32.0	32.0
6	34.72002222378548	36.0	36.0	36.0	32.0	36.0
7	34.667771347683086	36.0	36.0	36.0	32.0	36.0
8	34.65258193585251	36.0	36.0	36.0	32.0	36.0
9	34.71477379258662	36.0	36.0	36.0	32.0	36.0
10	34.53014495708139	36.0	36.0	36.0	32.0	36.0
11	34.69951546979331	36.0	36.0	36.0	32.0	36.0
12	34.606194234162324	36.0	36.0	36.0	32.0	36.0
13	34.633723589514624	36.0	36.0	36.0	32.0	36.0
14	34.57983981072456	36.0	36.0	36.0	32.0	36.0
15	34.55502612156181	36.0	36.0	36.0	32.0	36.0
16	34.551658385387434	36.0	36.0	36.0	32.0	36.0
17	34.515740843096914	36.0	36.0	36.0	32.0	36.0
18	34.51347482093917	36.0	36.0	36.0	32.0	36.0
19	34.51625595254332	36.0	36.0	36.0	32.0	36.0
20	34.49333702772661	36.0	36.0	36.0	32.0	36.0
21	34.48139963907883	36.0	36.0	36.0	32.0	36.0
22	34.459739774976995	36.0	36.0	36.0	32.0	36.0
23	34.4307334279902	36.0	36.0	36.0	32.0	36.0
24	34.41623082875482	36.0	36.0	36.0	32.0	36.0
25	34.10243585896797	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	48.0
5	285.0
6	823.0
7	148.0
8	683.0
9	489.0
10	259.0
11	76.0
12	154.0
13	154.0
14	518.0
15	762.0
16	1253.0
17	1620.0
18	1996.0
19	2548.0
20	3440.0
21	4905.0
22	6993.0
23	10313.0
24	14810.0
25	21353.0
26	29313.0
27	38794.0
28	51716.0
29	70130.0
30	92238.0
31	127893.0
32	187146.0
33	278466.0
34	676676.0
35	1856752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74580708108066	16.83148530299763	12.100552528385165	25.32215508753654
2	16.555355840516377	19.698139970253305	38.085874914578746	25.66062927465157
3	19.174924542084153	24.01244309071948	28.175520625163664	28.6371117420327
4	13.464477434672514	15.583489213405322	34.32568204830147	36.62635130362069
5	14.846072580186508	37.014418721416334	32.61368982888145	15.525818869515712
6	34.10544003070957	34.33923848994411	17.15558329224549	14.39973818710083
7	30.85118859746706	30.845008316071663	19.6985402462799	18.60526284018137
8	29.05519011153812	32.631389525058204	18.71593036636166	19.597489997042018
9	28.49817184696068	14.941665272337017	17.856964202239322	38.70319867846298
10	17.03350718590302	27.68623497671407	30.56554176702797	24.714716070354942
11	36.64272410886843	22.06569475927421	21.508447278303695	19.78313385355367
12	25.18959210848277	24.125489776618444	27.97386094498889	22.711057169909896
13	29.92614852754206	20.561824372308486	24.684091312920817	24.827935787228636
14	24.130917499977002	19.425953055573444	25.141009217776876	31.302120226672674
15	25.517499761348596	26.823728045508233	22.566992037668967	25.091780155474204
16	25.619449093009866	26.52852353672249	22.72585487525346	25.126172495014192
17	24.087123049318226	26.32060139031403	24.643961673356415	24.948313887011324
18	24.475705199611916	26.247094757267597	25.31057529914837	23.966624743972115
19	26.057346454606233	25.097883553780914	25.35898301128959	23.485786980323265
20	26.86547688642939	24.616517469903414	24.84802968874756	23.669975954919643
21	26.865520643731838	24.601439872021594	24.503921293050944	24.02911819119562
22	26.928437746752394	24.259552398530243	24.466253111936588	24.34575674278078
23	26.00328075767799	24.41882653712234	24.8073388273188	24.770553877880864
24	25.07012872645083	24.94406946652733	24.86687038486875	25.11893142215309
25	25.357966778015907	24.761463257193753	25.035213969592256	24.845355995198084
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	409.0
1	409.0
2	848.5
3	1288.0
4	1288.0
5	1288.0
6	2436.0
7	3584.0
8	3584.0
9	3584.0
10	4118.0
11	4652.0
12	4652.0
13	4652.0
14	5795.5
15	6939.0
16	6939.0
17	6939.0
18	12854.0
19	18769.0
20	18769.0
21	18769.0
22	27196.0
23	35623.0
24	35623.0
25	35623.0
26	52807.0
27	69991.0
28	69991.0
29	69991.0
30	96951.5
31	123912.0
32	123912.0
33	123912.0
34	158090.5
35	192269.0
36	192269.0
37	192269.0
38	241460.0
39	290651.0
40	290651.0
41	290651.0
42	335191.0
43	379731.0
44	379731.0
45	379731.0
46	430384.5
47	481038.0
48	481038.0
49	481038.0
50	501158.0
51	521278.0
52	521278.0
53	521278.0
54	496053.5
55	470829.0
56	470829.0
57	470829.0
58	419432.0
59	368035.0
60	368035.0
61	368035.0
62	311155.0
63	254275.0
64	254275.0
65	254275.0
66	201440.5
67	148606.0
68	148606.0
69	148606.0
70	107812.5
71	67019.0
72	67019.0
73	67019.0
74	47617.5
75	28216.0
76	28216.0
77	28216.0
78	19156.0
79	10096.0
80	10096.0
81	10096.0
82	6534.5
83	2973.0
84	2973.0
85	2973.0
86	2014.5
87	1056.0
88	1056.0
89	1056.0
90	701.5
91	347.0
92	347.0
93	347.0
94	244.0
95	141.0
96	141.0
97	141.0
98	584.5
99	1028.0
100	1028.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010910902432126291
2	4.306935170576168E-4
3	5.455451216063146E-4
4	0.010824763728714768
5	0.03758518758856136
6	0.0691119530371789
7	0.11338724659070192
8	0.11582784318736174
9	0.12579121988196126
10	0.1408080671767035
11	0.14718233122915622
12	0.14052093816533176
13	0.13474964503675968
14	0.12228824594322599
15	0.14009024464827416
16	0.12581993278309844
17	0.1305862743718694
18	0.11858428169653047
19	0.10396941501770868
20	0.09926049923121208
21	0.09814069608686227
22	0.109453579134909
23	0.08817731939226274
24	0.09874366701074294
25	0.09509712856632178
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3482755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.506388317854434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.97269412675723	12.093169659133373
2	10.84808239996162	4.449099803953905
3	5.904022475451537	3.6321053255684825
4	4.00945362391293	3.2887765181754895
5	2.9149379149205408	2.9887424402898772
6	2.26169955234915	2.7827573567273536
7	1.7482142296056058	2.5094691898563903
8	1.4590205427075742	2.3935393449990587
9	1.171887605068774	2.162806406497865
>10	9.563053260606349	38.69661797229782
>50	0.8397446438984121	11.783456808703848
>100	0.2946801723069151	10.264219800311297
>500	0.008855005669377922	1.1802972012829436
>1k	0.003373335493066634	1.312450135776211
>5k	2.811112910888862E-4	0.4624920364261575
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8420	0.24176262757500885	No Hit
GTATCAACGCAGAGTACATGGGGGG	7626	0.21896458407209235	No Hit
GGTATCAACGCAGAGTACATGGGGG	4722	0.13558231916973776	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4663	0.13388825800264445	No Hit
GTATCAACGCAGAGTACATGGGGGA	3555	0.10207436354265516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.613870341152334E-5
2	0.0	0.0	0.0	0.0	1.4356450568587224E-4
3	0.0	0.0	0.0	0.0	1.4356450568587224E-4
4	0.0	0.0	0.0	0.0	1.722774068230467E-4
5	0.0	0.0	0.0	0.0	1.722774068230467E-4
6	0.0	0.0	0.0	0.0	4.881193193319656E-4
7	2.871290113717445E-5	0.0	0.0	0.0	4.881193193319656E-4
8	2.871290113717445E-5	0.0	0.0	0.0	4.881193193319656E-4
9	2.871290113717445E-5	0.0	0.0	0.0	5.455451216063146E-4
10	2.871290113717445E-5	0.0	0.0	0.0	5.455451216063146E-4
11	2.871290113717445E-5	0.0	0.0	0.0	5.455451216063146E-4
12	2.871290113717445E-5	0.0	0.0	0.0	5.455451216063146E-4
13	2.871290113717445E-5	0.0	0.0	0.0	6.029709238806635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2035	0.0	15.350065	1
TCGCGTA	40	0.0052733934	14.253032	11
ACGGGTC	40	0.005277422	14.251392	5
GTATCAA	6740	0.0	13.720747	1
GTACGCT	50	0.0015018597	13.298046	3
CGTTCAA	80	2.8675742E-5	11.875477	13
GCCGATC	65	8.0134853E-4	11.694797	8
GTAGGCG	130	2.6266207E-9	11.690591	18
ACCGTGT	210	0.0	11.311931	8
TATAGTT	135	4.7293724E-9	11.260358	5
GGTTATA	170	1.2732926E-11	11.17017	1
GCGTACT	60	0.005876379	11.083778	13
ACGGCGC	70	0.00148956	10.859453	10
CTTACAC	290	0.0	10.808758	3
TATTCCG	80	3.7698008E-4	10.688544	5
CCCGTCG	100	2.3943254E-5	10.452524	9
TGGACCG	240	0.0	10.292672	5
CAACGTT	120	1.528424E-6	10.290008	19
ATACCGA	130	3.8353392E-7	10.2328	6
CTAACAC	260	0.0	10.229266	3
>>END_MODULE