FastQCFastQC Report
Thu 2 Feb 2017
SRR4061962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482755
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67100.031.3840831
GGTATCA22250.030.6585581
TGCGATA701.1332068E-625.1430824
TATCAAC98000.021.4601822
TCGACGT755.476055E-520.53351435
GTCGACG550.004485047420.00017734
ATCAACG107500.019.6043473
TCAACGC108350.019.4302484
CAACGCA110200.019.1439865
CCCTATA1152.0251537E-719.1295032
AACGCAG113650.018.5628436
TTAGTCG1854.2200554E-1016.65046939
TATAGCG800.001990216.498965
CGGTAAG2151.2732926E-1116.37106116
CCTATAC2151.2732926E-1116.3710613
GCGATTT3250.016.2479339
TACCGTA1906.184564E-1016.2095057
TAACGCC850.002982698115.5284344
TATACCG1704.501453E-815.5284345
ACGCAGA136300.015.4781167
Produced by FastQC (version 0.11.5)