##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3482755
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313479702132362	32.0	32.0	32.0	32.0	32.0
2	31.564783626755254	32.0	32.0	32.0	32.0	32.0
3	31.634918907588965	32.0	32.0	32.0	32.0	32.0
4	31.692785740024778	32.0	32.0	32.0	32.0	32.0
5	31.683809512871274	32.0	32.0	32.0	32.0	32.0
6	35.34492521007076	36.0	36.0	36.0	36.0	36.0
7	35.34970188830394	36.0	36.0	36.0	36.0	36.0
8	35.31218561167811	36.0	36.0	36.0	36.0	36.0
9	35.36534094416633	36.0	36.0	36.0	36.0	36.0
10	35.27952325098952	36.0	36.0	36.0	36.0	36.0
11	35.369298156201054	36.0	36.0	36.0	36.0	36.0
12	35.318299736846264	36.0	36.0	36.0	36.0	36.0
13	35.33110339372135	36.0	36.0	36.0	36.0	36.0
14	35.30030852012272	36.0	36.0	36.0	36.0	36.0
15	35.29144657031574	36.0	36.0	36.0	36.0	36.0
16	35.30408397949325	36.0	36.0	36.0	36.0	36.0
17	35.2885847554594	36.0	36.0	36.0	36.0	36.0
18	35.2815704808406	36.0	36.0	36.0	36.0	36.0
19	35.27362102703176	36.0	36.0	36.0	36.0	36.0
20	35.27173860923321	36.0	36.0	36.0	36.0	36.0
21	35.26747445628533	36.0	36.0	36.0	36.0	36.0
22	35.24817364414092	36.0	36.0	36.0	36.0	36.0
23	35.22107325953161	36.0	36.0	36.0	36.0	36.0
24	35.20909911837037	36.0	36.0	36.0	36.0	36.0
25	35.20348029074684	36.0	36.0	36.0	36.0	36.0
26	35.16403508142261	36.0	36.0	36.0	36.0	36.0
27	35.148415550333	36.0	36.0	36.0	36.0	36.0
28	35.13696168694037	36.0	36.0	36.0	36.0	36.0
29	35.12536512042909	36.0	36.0	36.0	36.0	36.0
30	35.10726048774605	36.0	36.0	36.0	36.0	36.0
31	35.10281171084386	36.0	36.0	36.0	36.0	36.0
32	35.08201868922735	36.0	36.0	36.0	36.0	36.0
33	35.060342745900876	36.0	36.0	36.0	36.0	36.0
34	35.05555687953933	36.0	36.0	36.0	36.0	36.0
35	35.04302714374109	36.0	36.0	36.0	36.0	36.0
36	35.02296888526468	36.0	36.0	36.0	36.0	36.0
37	35.012148141342124	36.0	36.0	36.0	36.0	36.0
38	35.009454009828424	36.0	36.0	36.0	36.0	36.0
39	34.990260871063285	36.0	36.0	36.0	36.0	36.0
40	34.97698172854536	36.0	36.0	36.0	36.0	36.0
41	34.95151998920395	36.0	36.0	36.0	36.0	36.0
42	34.93728987540037	36.0	36.0	36.0	36.0	36.0
43	34.920074768394564	36.0	36.0	36.0	36.0	36.0
44	34.893359136660486	36.0	36.0	36.0	36.0	36.0
45	34.873980512554	36.0	36.0	36.0	36.0	36.0
46	34.85123903346632	36.0	36.0	36.0	36.0	36.0
47	34.82370709395292	36.0	36.0	36.0	36.0	36.0
48	34.81265377553115	36.0	36.0	36.0	36.0	36.0
49	34.79546967845857	36.0	36.0	36.0	36.0	36.0
50	34.410249070060914	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	24.0
21	87.0
22	221.0
23	584.0
24	1594.0
25	3748.0
26	7807.0
27	14512.0
28	24666.0
29	39066.0
30	57786.0
31	84308.0
32	124898.0
33	206509.0
34	475691.0
35	2441245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03338443978122	17.486619742177638	12.54782971844732	25.93216609959381
2	15.98321938557223	19.98110601592493	37.78592943757376	26.249745160929077
3	18.551532097410604	24.278226753517774	28.45562490704164	28.71461624202999
4	13.195878893542295	15.86827550840513	34.295732629981906	36.64011296807067
5	14.517140468227424	37.23009849682091	32.57323518872432	15.679525846227351
6	34.303161930269965	34.35941064895344	16.99133384978405	14.346093570992544
7	30.844460778894867	30.749823056746745	19.70744425031333	18.69827191404506
8	29.15169169235275	32.596636857889806	18.667319406619185	19.58435204313826
9	28.51059003576192	14.656787514482069	17.860601736269132	38.972020713486884
10	16.964476962771418	27.628910913604578	30.670584256022675	24.73602786760133
11	36.82050560547612	21.807850394299916	21.532005553075077	19.83963844714888
12	25.172944981774485	23.994768509412808	28.101747036469693	22.73053947234302
13	30.127470924598487	20.311879532151988	24.74937226419889	24.811277279050636
14	24.164576606738056	19.2360358394432	25.18109944569744	31.4182881081213
15	25.593273141521582	26.76943971080366	22.516714497574476	25.120572650100282
16	25.678764278326803	26.300719608720296	22.762370625768437	25.258145487184464
17	24.172616219056465	26.152428178266916	24.709978163838684	24.96497743883793
18	24.641389706410163	26.041576340535492	25.335812217356974	23.981221735697368
19	26.152514316970333	24.949013065805662	25.38863629511694	23.509836322107066
20	27.03370473577595	24.453039197529534	24.78191771146036	23.73133835523416
21	26.951810823219446	24.467569190235423	24.467971171082187	24.11264881546294
22	27.03982910081243	24.175745925280417	24.47763336783667	24.306791606070483
23	26.133550096719464	24.244010413600964	24.752631036424347	24.869808453255228
24	25.217767821328312	24.718790440698267	24.871113780797383	25.192327957176037
25	25.55306167627928	24.488586458570513	25.01498098197717	24.94337088317303
26	25.16573157915894	24.885837013386013	24.912080718055325	25.03635068939973
27	25.225390260591023	25.414006282989497	24.4841054607339	24.876497995685575
28	25.73271561247827	24.78356560077756	24.692687703057544	24.791031083686626
29	25.32323053373918	25.19772557800186	24.613873422897147	24.86517046536181
30	25.617765695239804	24.96293118696005	24.574814110382942	24.84448900741721
31	25.341786472106655	24.734676532490315	24.896733309788583	25.026803685614446
32	25.470342366575178	25.009123592447185	24.860274801743117	24.66025923923452
33	25.59041055534914	24.776885825150615	24.688478574090674	24.94422504540957
34	25.250543467493454	24.682540616855345	25.126617661035354	24.940298254615847
35	25.140614578090226	24.83476405262857	24.99170913075225	25.032912238528954
36	25.453894453460315	24.932093852325806	24.92577700137937	24.68823469283451
37	25.707380578561278	24.500532344371102	24.988974097168065	24.80311297989955
38	25.805784732427266	24.94660834500513	24.53690384104074	24.710703081526862
39	25.413433921350574	25.010788977928467	24.65351086119158	24.922266239529378
40	25.362500689110224	24.965774192362822	24.45066430225293	25.221060816274026
41	25.19043310700634	25.050370127477983	24.676380724370812	25.082816041144863
42	25.12230132369988	24.76024288840451	24.841073604191365	25.27638218370424
43	25.3332123514119	24.50662060165152	24.97529154824508	25.1848754986915
44	25.188644057041014	24.522507068678525	24.900958101335863	25.387890772944598
45	25.213528945928203	24.653670492662805	24.825320602090468	25.307479959318524
46	25.509305759182148	24.379630411274988	24.653758224265093	25.457305605277767
47	25.060132752904273	24.938359800741434	24.97677814782794	25.02472929852635
48	25.08950899196034	24.943009713114673	24.679041098761818	25.288440196163165
49	25.252288289602404	24.685244426399297	24.656932432824338	25.405534851173954
50	25.01027936913387	25.054239464508036	24.690814952979064	25.24466621337903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	137.0
1	174.5
2	212.0
3	460.5
4	709.0
5	835.0
6	961.0
7	860.5
8	760.0
9	992.0
10	1224.0
11	1739.0
12	2254.0
13	3691.0
14	5128.0
15	6404.0
16	7680.0
17	6852.5
18	6025.0
19	5169.5
20	4314.0
21	4686.0
22	5058.0
23	6035.0
24	7012.0
25	8483.0
26	9954.0
27	13221.5
28	16489.0
29	20382.0
30	24275.0
31	30878.0
32	37481.0
33	46003.5
34	54526.0
35	67390.0
36	80254.0
37	90451.5
38	100649.0
39	119500.5
40	138352.0
41	160201.5
42	182051.0
43	199147.0
44	216243.0
45	234740.0
46	253237.0
47	267583.0
48	281929.0
49	288684.0
50	295439.0
51	303792.0
52	312145.0
53	308594.5
54	305044.0
55	293059.5
56	281075.0
57	262391.0
58	243707.0
59	219195.5
60	194684.0
61	167552.5
62	140421.0
63	121776.0
64	103131.0
65	86634.0
66	70137.0
67	57737.5
68	45338.0
69	34551.5
70	23765.0
71	18196.5
72	12628.0
73	10363.5
74	8099.0
75	6427.0
76	4755.0
77	3695.0
78	2635.0
79	2059.5
80	1484.0
81	1096.5
82	709.0
83	524.5
84	340.0
85	262.5
86	185.0
87	120.0
88	55.0
89	38.5
90	22.0
91	16.5
92	11.0
93	12.5
94	14.0
95	11.0
96	8.0
97	8.5
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032531716988418655
2	0.004737628687633784
3	3.7326771478326783E-4
4	2.8712901137174452E-5
5	8.613870341152336E-5
6	2.5841611023457003E-4
7	0.0
8	0.0
9	0.0
10	2.8712901137174452E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.326741329780591E-4
17	0.0
18	1.4356450568587224E-4
19	0.0
20	2.871290113717445E-4
21	5.7425802274348903E-5
22	0.0
23	5.7425802274348903E-5
24	9.188128363895825E-4
25	1.7227740682304672E-4
26	4.306935170576168E-4
27	5.168322204691401E-4
28	0.0017227740682304671
29	6.891096272921869E-4
30	0.0012346547488985013
31	7.178225284293612E-4
32	7.465354295665357E-4
33	2.5841611023457003E-4
34	7.465354295665357E-4
35	2.2970320909739561E-4
36	2.0099030796022114E-4
37	0.0013495063534471991
38	2.8712901137174452E-5
39	9.762386386639314E-4
40	8.613870341152336E-5
41	0.0010336644409382801
42	0.004823767391045308
43	0.004048519060341598
44	0.003962380356930074
45	0.002555448201208526
46	0.002096041783013735
47	0.0012633676500356757
48	0.0041346577637531206
49	0.003790102950107028
50	0.001464357957995897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3482755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.302447349324506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.551874371118515	12.47298220995015
2	11.370542749258139	4.8444077649862995
3	6.087269401477086	3.890212077783592
4	3.9110682101298164	3.3326129850362896
5	2.7725331603284604	2.953087083607665
6	2.191777807139773	2.8014138802807755
7	1.7403169849544147	2.595110766018861
8	1.4452166540048934	2.4629321344244937
9	1.189678396491709	2.2808755263504086
>10	9.664546937897466	40.65079188834374
>50	0.8064355285449529	11.68771786292945
>100	0.263858094749002	9.249069486432825
>500	0.004203689474418094	0.5424061940275233
>1k	6.780144313259917E-4	0.23638013982796402
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.74258022743489E-5
2	0.0	0.0	0.0	0.0	5.74258022743489E-5
3	0.0	0.0	0.0	0.0	5.74258022743489E-5
4	0.0	0.0	0.0	0.0	8.613870341152334E-5
5	0.0	0.0	0.0	0.0	8.613870341152334E-5
6	0.0	0.0	0.0	0.0	3.445548136460934E-4
7	0.0	0.0	0.0	0.0	3.445548136460934E-4
8	0.0	0.0	0.0	0.0	4.881193193319656E-4
9	0.0	0.0	0.0	0.0	6.891096272921868E-4
10	0.0	0.0	0.0	0.0	6.891096272921868E-4
11	0.0	0.0	0.0	0.0	6.891096272921868E-4
12	0.0	0.0	0.0	0.0	6.891096272921868E-4
13	0.0	0.0	0.0	0.0	6.891096272921868E-4
14	0.0	0.0	0.0	0.0	8.326741329780591E-4
15	0.0	0.0	0.0	2.871290113717445E-5	0.0010910902432126291
16	0.0	0.0	0.0	2.0099030796022114E-4	0.0012920805511728503
17	0.0	0.0	0.0	2.0099030796022114E-4	0.0013207934523100247
18	0.0	0.0	0.0	3.1584191250891893E-4	0.001464357957995897
19	0.0	0.0	0.0	4.019806159204423E-4	0.001464357957995897
20	0.0	0.0	0.0	6.029709238806635E-4	0.0014930708591330713
21	0.0	0.0	0.0	8.90099935252408E-4	0.0016366353648189437
22	0.0	0.0	0.0	0.0012920805511728503	0.0016366353648189437
23	0.0	0.0	0.0	0.001722774068230467	0.0018950514750535137
24	0.0	0.0	0.0	0.002555448201208526	0.0020099030796022115
25	0.0	0.0	0.0	0.003388122334186585	0.002038615980739386
26	0.0	0.0	0.0	0.004163370664890295	0.0022108933875624325
27	0.0	0.0	0.0	0.005857431831983588	0.002239606288699607
28	0.0	0.0	0.0	0.007063373679744914	0.002239606288699607
29	0.0	0.0	0.0	0.009073276759347127	0.0022683191898367817
30	0.0	0.0	0.0	0.011887141070790221	0.0022683191898367817
31	0.0	0.0	0.0	0.020414872708531033	0.0023257449921111305
32	0.0	0.0	0.0	0.029660426874701207	0.0026702998057572236
33	0.0	0.0	0.0	0.03950895196475204	0.0026990127068943983
34	0.0	0.0	0.0	0.05148223173895379	0.002928715915991794
35	0.0	0.0	0.0	0.06268026318245182	0.002928715915991794
36	0.0	0.0	0.0	0.08025255867840259	0.003043567520540492
37	0.0	0.0	0.0	0.10841991469397072	0.003043567520540492
38	0.0	0.0	0.0	0.14428232821430162	0.003129706223952015
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6710	0.0	31.384083	1
GGTATCA	2225	0.0	30.658558	1
TGCGATA	70	1.1332068E-6	25.14308	24
TATCAAC	9800	0.0	21.460182	2
TCGACGT	75	5.476055E-5	20.533514	35
GTCGACG	55	0.0044850474	20.000177	34
ATCAACG	10750	0.0	19.604347	3
TCAACGC	10835	0.0	19.430248	4
CAACGCA	11020	0.0	19.143986	5
CCCTATA	115	2.0251537E-7	19.129503	2
AACGCAG	11365	0.0	18.562843	6
TTAGTCG	185	4.2200554E-10	16.650469	39
TATAGCG	80	0.0019902	16.49896	5
CGGTAAG	215	1.2732926E-11	16.371061	16
CCTATAC	215	1.2732926E-11	16.371061	3
GCGATTT	325	0.0	16.24793	39
TACCGTA	190	6.184564E-10	16.209505	7
TAACGCC	85	0.0029826981	15.528434	4
TATACCG	170	4.501453E-8	15.528434	5
ACGCAGA	13630	0.0	15.478116	7
>>END_MODULE