FastQCFastQC Report
Thu 2 Feb 2017
SRR4061961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5831840
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76470.13112499657055063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.014.7316531
GTATCAA58500.012.5325561
TCGTATA853.9557763E-612.2921122
GTACGTA550.00306742412.09236311
GTATAGG8050.010.8535091
ATAGGGG5850.09.9053883
TATAGGG6850.09.845112
CCGTGCG2750.09.6738069
GTACTAA2950.09.6577831
CGCGTAA1106.834371E-59.501142510
CGTCCGA2900.09.50114211
TCGCGTA1106.835013E-59.50106059
GTTCTAG5300.09.496821
GCGTTAT1606.681876E-89.496821
TCTTATA4700.09.2963562
TGCGCGA1252.753177E-59.12109610
GTATTAC3750.09.1169471
ACCGTGC3450.09.0877378
TTACACC9100.09.0823394
CCTACAC8050.08.9684053
Produced by FastQC (version 0.11.5)