##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061961_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5831840
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300220685066805	32.0	32.0	32.0	32.0	32.0
2	30.880042147932727	32.0	32.0	32.0	32.0	32.0
3	30.90402943153447	32.0	32.0	32.0	32.0	32.0
4	30.908472969080083	32.0	32.0	32.0	32.0	32.0
5	30.837216898954704	32.0	32.0	32.0	32.0	32.0
6	34.48227351916376	36.0	36.0	36.0	32.0	36.0
7	34.433101388241106	36.0	36.0	36.0	32.0	36.0
8	34.39800748991742	36.0	36.0	36.0	32.0	36.0
9	34.52349464320009	36.0	36.0	36.0	32.0	36.0
10	34.24990397541771	36.0	36.0	36.0	32.0	36.0
11	34.501588520947074	36.0	36.0	36.0	32.0	36.0
12	34.35181589344015	36.0	36.0	36.0	32.0	36.0
13	34.41730020027984	36.0	36.0	36.0	32.0	36.0
14	34.34142980603034	36.0	36.0	36.0	32.0	36.0
15	34.299180876018546	36.0	36.0	36.0	32.0	36.0
16	34.298073506817744	36.0	36.0	36.0	32.0	36.0
17	34.254800028807374	36.0	36.0	36.0	32.0	36.0
18	34.2464983607232	36.0	36.0	36.0	32.0	36.0
19	34.24863147822985	36.0	36.0	36.0	32.0	36.0
20	34.237032394578726	36.0	36.0	36.0	32.0	36.0
21	34.21983010507833	36.0	36.0	36.0	32.0	36.0
22	34.190031962468105	36.0	36.0	36.0	32.0	36.0
23	34.1575005487119	36.0	36.0	36.0	32.0	36.0
24	34.136149825783974	36.0	36.0	36.0	32.0	36.0
25	33.789202893083484	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	66.0
5	505.0
6	1202.0
7	265.0
8	954.0
9	811.0
10	426.0
11	119.0
12	230.0
13	256.0
14	609.0
15	932.0
16	1463.0
17	2245.0
18	3111.0
19	4297.0
20	6652.0
21	9647.0
22	14330.0
23	21514.0
24	30952.0
25	43967.0
26	61096.0
27	80177.0
28	106903.0
29	143842.0
30	190237.0
31	259762.0
32	371638.0
33	531695.0
34	1191435.0
35	2750500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3831856854707	16.979049796280073	11.856463745809386	25.78130077243984
2	16.81111527218205	19.180233586975756	37.62874170586532	26.379909434976867
3	19.425227005462123	22.693207137832964	28.044164081129008	29.8374017755759
4	13.408152426395775	15.410117366500087	34.44031704509227	36.74141316201187
5	15.173829217168166	35.960777788831976	33.40498742679927	15.460405567200585
6	35.10417907057108	34.22659175194547	16.566045622118075	14.103183555365367
7	31.173472085184628	30.01639988107769	19.822158961778012	18.98796907195967
8	28.301342670585495	33.62718807515989	18.571023459743056	19.500445794511563
9	27.143278236597734	15.233957548600705	18.253820150397416	39.36894406440415
10	16.426866212877833	27.242971847556074	31.346421828566168	24.98374011099993
11	36.46194930236839	21.759107262319652	21.97596237720873	19.802981058103228
12	24.52383699607284	23.572678559409457	28.608704998715673	23.294779445802032
13	29.556294597174627	20.128958374825732	24.906118440481016	25.408628587518628
14	23.535268774969545	19.25180288874209	25.266484878532474	31.94644345775589
15	25.06964596583952	27.293680962497373	22.04121186813617	25.59546120352693
16	25.493716830673836	26.33703511869067	23.341883486381704	24.82736456425379
17	24.090670138792316	26.443900551344164	24.910407003035708	24.555022306827816
18	24.33060954241789	26.263853919524312	25.38443698622071	24.021099551837086
19	25.50829502326067	25.44945679337826	25.160672307447257	23.881575875913814
20	25.668127776481953	25.096281696068218	25.025586155963047	24.210004371486786
21	25.832687719235615	24.859323634418914	24.81286344755525	24.495125198790216
22	25.881994094950084	24.556370294450897	24.92793769024012	24.633697920358895
23	25.1102575046237	24.96814420904195	25.02134445252745	24.9002538338069
24	24.80147383409	25.182049541091672	25.00351844592475	25.01295817889358
25	24.88152134606648	25.041169111812987	25.121885456932947	24.95542408518758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	233.0
1	233.0
2	741.0
3	1249.0
4	1249.0
5	1249.0
6	2569.5
7	3890.0
8	3890.0
9	3890.0
10	4826.0
11	5762.0
12	5762.0
13	5762.0
14	8195.5
15	10629.0
16	10629.0
17	10629.0
18	20939.5
19	31250.0
20	31250.0
21	31250.0
22	47992.0
23	64734.0
24	64734.0
25	64734.0
26	98807.0
27	132880.0
28	132880.0
29	132880.0
30	182442.0
31	232004.0
32	232004.0
33	232004.0
34	291109.0
35	350214.0
36	350214.0
37	350214.0
38	425819.0
39	501424.0
40	501424.0
41	501424.0
42	573574.0
43	645724.0
44	645724.0
45	645724.0
46	708849.5
47	771975.0
48	771975.0
49	771975.0
50	796821.0
51	821667.0
52	821667.0
53	821667.0
54	784452.5
55	747238.0
56	747238.0
57	747238.0
58	675068.5
59	602899.0
60	602899.0
61	602899.0
62	515688.0
63	428477.0
64	428477.0
65	428477.0
66	341921.0
67	255365.0
68	255365.0
69	255365.0
70	190888.0
71	126411.0
72	126411.0
73	126411.0
74	91886.5
75	57362.0
76	57362.0
77	57362.0
78	41275.5
79	25189.0
80	25189.0
81	25189.0
82	17056.5
83	8924.0
84	8924.0
85	8924.0
86	5978.0
87	3032.0
88	3032.0
89	3032.0
90	2020.5
91	1009.0
92	1009.0
93	1009.0
94	699.5
95	390.0
96	390.0
97	390.0
98	1149.5
99	1909.0
100	1909.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010116875634448132
2	1.3717797470438146E-4
3	7.201843671980027E-4
4	0.010614145792751516
5	0.03395154873933441
6	0.06347910779445252
7	0.10610716343383907
8	0.10778759362396774
9	0.11785302751790172
10	0.13189662267826277
11	0.13951000027435595
12	0.13347416938736317
13	0.12826140634859667
14	0.11564103267579356
15	0.13273683777332712
16	0.11991069713846744
17	0.1228771708414497
18	0.11150854618782408
19	0.0976707179895196
20	0.09340105352684573
21	0.09166918159620291
22	0.10252338884468709
23	0.08228963757579083
24	0.09266372191280968
25	0.09024596010864495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5831840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.65314634825163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52716243857019	31.04606559548138
2	14.974555410213622	13.373220284645443
3	5.750079426559008	7.702774144446306
4	2.8307480110639807	5.056072208522487
5	1.6588918159627306	3.7037369517050363
6	1.0888437650957326	2.917217999592064
7	0.7437181007130758	2.3246547235089303
8	0.5363817503099181	1.916090623609611
9	0.4116979788619941	1.6545249091263627
>10	2.2555819107565744	18.10017851054459
>50	0.1455663479153853	4.4786869153133475
>100	0.07221850311380058	5.6972442221249375
>500	0.003242226070620674	0.9324737461408297
>1k	0.001273717299600464	0.9652631550697433
>5k	3.859749391614888E-5	0.13179601016895104
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7647	0.13112499657055063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7147246838047683E-5	0.0	0.0	0.0	6.858898735219073E-5
2	1.7147246838047683E-5	0.0	0.0	0.0	6.858898735219073E-5
3	1.7147246838047683E-5	0.0	0.0	0.0	6.858898735219073E-5
4	1.7147246838047683E-5	0.0	0.0	0.0	6.858898735219073E-5
5	1.7147246838047683E-5	0.0	0.0	0.0	8.573623419023842E-5
6	1.7147246838047683E-5	0.0	0.0	0.0	8.573623419023842E-5
7	1.7147246838047683E-5	0.0	0.0	0.0	8.573623419023842E-5
8	1.7147246838047683E-5	0.0	0.0	0.0	1.028834810282861E-4
9	1.7147246838047683E-5	0.0	0.0	0.0	1.2003072786633378E-4
10	1.7147246838047683E-5	0.0	0.0	1.7147246838047683E-5	1.5432522154242915E-4
11	1.7147246838047683E-5	0.0	0.0	3.4294493676095365E-5	1.5432522154242915E-4
12	1.7147246838047683E-5	0.0	0.0	3.4294493676095365E-5	3.4294493676095367E-4
13	1.7147246838047683E-5	0.0	0.0	3.4294493676095365E-5	4.115339241131444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2050	0.0	14.731653	1
GTATCAA	5850	0.0	12.532556	1
TCGTATA	85	3.9557763E-6	12.292112	2
GTACGTA	55	0.003067424	12.092363	11
GTATAGG	805	0.0	10.853509	1
ATAGGGG	585	0.0	9.905388	3
TATAGGG	685	0.0	9.84511	2
CCGTGCG	275	0.0	9.673806	9
GTACTAA	295	0.0	9.657783	1
CGCGTAA	110	6.834371E-5	9.5011425	10
CGTCCGA	290	0.0	9.501142	11
TCGCGTA	110	6.835013E-5	9.5010605	9
GTTCTAG	530	0.0	9.49682	1
GCGTTAT	160	6.681876E-8	9.49682	1
TCTTATA	470	0.0	9.296356	2
TGCGCGA	125	2.753177E-5	9.121096	10
GTATTAC	375	0.0	9.116947	1
ACCGTGC	345	0.0	9.087737	8
TTACACC	910	0.0	9.082339	4
CCTACAC	805	0.0	8.968405	3
>>END_MODULE