FastQCFastQC Report
Thu 2 Feb 2017
SRR4061961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5831840
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.028.3880021
GTATCAA63000.027.8064751
TCAACGC92450.018.6082554
TATCAAC93000.018.5463122
GTATAGG10450.018.3221471
ATCAACG95000.018.1550853
CAACGCA95300.018.0517675
AACGCAG99650.017.3520626
TATACCG2750.015.199415
ATAGGGG9350.014.8229543
TATAGGG11100.014.6667262
TAGGGCG6850.014.45236631
CGCGTAA3200.014.4369410
ACGCAGA121050.014.2844527
TACGCGG1100.001019118413.99945710
AGAGTAC125800.013.78006811
ATACCGT5200.013.5379386
GTACTAG4400.013.5046861
ACCGTAT3151.8189894E-1213.2693268
GTCTTAG9250.013.0856221
Produced by FastQC (version 0.11.5)