##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5831840
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280599090510027	32.0	32.0	32.0	32.0	32.0
2	31.437881697714616	32.0	32.0	32.0	32.0	32.0
3	31.560318527257262	32.0	32.0	32.0	32.0	32.0
4	31.643707303355374	32.0	32.0	32.0	32.0	32.0
5	31.615112383055777	32.0	32.0	32.0	32.0	32.0
6	35.25656396608961	36.0	36.0	36.0	36.0	36.0
7	35.265852629701776	36.0	36.0	36.0	36.0	36.0
8	35.21637819281736	36.0	36.0	36.0	36.0	36.0
9	35.30420210431013	36.0	36.0	36.0	36.0	36.0
10	35.183732749869684	36.0	36.0	36.0	36.0	36.0
11	35.300928694888746	36.0	36.0	36.0	36.0	36.0
12	35.23174229745672	36.0	36.0	36.0	36.0	36.0
13	35.263430924030835	36.0	36.0	36.0	36.0	36.0
14	35.23007764273368	36.0	36.0	36.0	36.0	36.0
15	35.20648285961206	36.0	36.0	36.0	36.0	36.0
16	35.22457423386101	36.0	36.0	36.0	36.0	36.0
17	35.20901756563966	36.0	36.0	36.0	36.0	36.0
18	35.199765768608195	36.0	36.0	36.0	36.0	36.0
19	35.19296774259925	36.0	36.0	36.0	36.0	36.0
20	35.193442549864194	36.0	36.0	36.0	36.0	36.0
21	35.18737499657055	36.0	36.0	36.0	36.0	36.0
22	35.16769407253971	36.0	36.0	36.0	36.0	36.0
23	35.140541235699196	36.0	36.0	36.0	36.0	36.0
24	35.13159105873961	36.0	36.0	36.0	36.0	36.0
25	35.120170649400535	36.0	36.0	36.0	36.0	36.0
26	35.0743758402151	36.0	36.0	36.0	36.0	36.0
27	35.062733202557	36.0	36.0	36.0	36.0	36.0
28	35.045177851244205	36.0	36.0	36.0	36.0	36.0
29	35.03143433290351	36.0	36.0	36.0	36.0	36.0
30	35.01281568081429	36.0	36.0	36.0	36.0	36.0
31	35.010750637877585	36.0	36.0	36.0	36.0	36.0
32	34.9856817059453	36.0	36.0	36.0	36.0	36.0
33	34.97055594803698	36.0	36.0	36.0	36.0	36.0
34	34.96425484924141	36.0	36.0	36.0	36.0	36.0
35	34.95213020247469	36.0	36.0	36.0	36.0	36.0
36	34.92568811901561	36.0	36.0	36.0	36.0	36.0
37	34.921697440258995	36.0	36.0	36.0	36.0	36.0
38	34.91263837828198	36.0	36.0	36.0	36.0	36.0
39	34.89224018491591	36.0	36.0	36.0	36.0	36.0
40	34.88191479875991	36.0	36.0	36.0	32.0	36.0
41	34.860225074762	36.0	36.0	36.0	32.0	36.0
42	34.84081370545145	36.0	36.0	36.0	32.0	36.0
43	34.82803008998875	36.0	36.0	36.0	32.0	36.0
44	34.8011903618755	36.0	36.0	36.0	32.0	36.0
45	34.78780213448928	36.0	36.0	36.0	32.0	36.0
46	34.77159970095202	36.0	36.0	36.0	32.0	36.0
47	34.747746337348076	36.0	36.0	36.0	32.0	36.0
48	34.727819864742514	36.0	36.0	36.0	32.0	36.0
49	34.71311078493237	36.0	36.0	36.0	32.0	36.0
50	34.31107111992099	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	29.0
21	150.0
22	374.0
23	1026.0
24	2704.0
25	6314.0
26	13449.0
27	25294.0
28	43573.0
29	69286.0
30	103976.0
31	154860.0
32	236117.0
33	400397.0
34	921082.0
35	3853198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.949281881541886	17.53997022655298	12.280629232077313	26.230118659827816
2	16.27972897127509	19.665787484958997	37.27630981698033	26.77817372678558
3	18.77120476502557	23.12359799486473	28.366827130987794	29.73837010912191
4	13.191977146149414	15.857585256111278	34.52037435869297	36.43006323904634
5	14.947274779019104	36.2915025603416	33.18797038121251	15.57325227942678
6	35.329482397374825	34.329264455456425	16.452745785241646	13.888507361927108
7	31.22570235123049	30.052179072128176	19.828699004087905	18.89341957255343
8	28.386289747318173	33.72357609262257	18.46849364865977	19.42164051139949
9	27.282212817909958	14.992386622403908	18.216840654064583	39.50855990562155
10	16.325199075351385	27.288845693046632	31.458166611995498	24.92778861960648
11	36.746189881752585	21.61844289280913	21.980198359351423	19.65516886608686
12	24.63236725156507	23.516715739237657	28.562482606258506	23.288434402938766
13	29.66729196960136	20.00613871436802	24.9330742955911	25.393495020439516
14	23.65575873137809	19.085674504101622	25.276516502510354	31.98205026200993
15	25.140350215369423	27.25695492331751	21.994070482043405	25.608624379269664
16	25.643140919052733	26.26454330037215	23.333737438931898	24.75857834164322
17	24.230448709155258	26.366018272106228	24.8880970671349	24.51543595160361
18	24.56561211435113	26.144516975555003	25.367179101849214	23.922691808244657
19	25.629081044747455	25.38925965047052	25.153570742681552	23.82808856210047
20	25.864811647960956	25.002662119657693	24.987984023509647	24.14454220887171
21	26.034052037444795	24.73254148477007	24.80858953753696	24.424816940248178
22	26.0338075118659	24.499437570303712	24.895504677768937	24.571250240061456
23	25.291168924788376	24.80790790141976	25.02530077138631	24.875622402405554
24	24.98553614352261	25.055857750415058	24.990183096818843	24.968423009243494
25	25.072481561527532	24.818033042126757	25.21176893420039	24.89771646214532
26	24.920864967341153	25.176823500926986	25.272522876283222	24.62978865544864
27	25.059895497532164	25.446275470590336	24.861758516717924	24.63207051515958
28	25.26906547407052	24.828203634615218	25.183773883660145	24.718957007654122
29	25.01496531869913	25.186661378434888	25.037239680188865	24.761133622677118
30	25.00498561929622	25.289496007474916	24.976092176427304	24.72942619680156
31	25.2094543384398	24.940738911694805	24.88427283420956	24.965533915655833
32	25.12796251439953	25.243553175648074	24.889116301592683	24.7393680083597
33	25.012461802239816	25.20221385395745	24.85057411741547	24.934750226387266
34	25.23476921583781	24.923063943821035	25.162132867930282	24.68003397241088
35	25.034877571835107	25.16091009542805	25.18952890928882	24.614683423448017
36	24.984593172297544	25.149995798917317	25.142348113713876	24.723062915071257
37	25.423210317891726	24.826115182894146	25.0429632078555	24.707711291358624
38	25.15950800425046	25.08008194327724	25.053160761125266	24.707249291347036
39	25.169767539959377	25.2516462332886	24.937403524607678	24.64118270214435
40	25.301020124708586	25.009705348367135	24.95639452138229	24.732880005541993
41	24.996754848479288	25.152162025399804	25.081720374424464	24.769362751696438
42	25.104594833277606	25.030755116564208	25.01485882827036	24.849791221887834
43	25.080411270833324	24.750929550346793	25.097696457377012	25.070962721442875
44	24.9408266520566	25.005251564966358	25.08879646134548	24.96512532163156
45	24.951373552459717	25.13768276608051	25.036700257197598	24.874243424262175
46	25.168120305496505	24.885423377489225	24.956723500421063	24.989732816593207
47	25.018763622595774	25.18490549084385	24.99083038868349	24.805500497876885
48	24.939810645319053	25.42586969330523	24.782563812719538	24.851755848656182
49	25.04595199728927	25.095697960209744	24.93145568759604	24.92689435490495
50	24.84162105278681	25.351160142400015	24.92156287462012	24.88565593019306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	42.5
2	71.0
3	182.5
4	294.0
5	357.0
6	420.0
7	410.0
8	400.0
9	505.0
10	610.0
11	918.0
12	1226.0
13	2448.0
14	3670.0
15	4796.0
16	5922.0
17	5796.5
18	5671.0
19	5655.0
20	5639.0
21	6814.5
22	7990.0
23	11028.5
24	14067.0
25	18978.0
26	23889.0
27	31884.5
28	39880.0
29	49050.5
30	58221.0
31	73757.0
32	89293.0
33	107596.5
34	125900.0
35	148596.0
36	171292.0
37	190979.5
38	210667.0
39	239123.5
40	267580.0
41	292444.0
42	317308.0
43	334755.5
44	352203.0
45	376705.0
46	401207.0
47	419621.5
48	438036.0
49	448867.0
50	459698.0
51	466823.5
52	473949.0
53	471442.0
54	468935.0
55	454141.5
56	439348.0
57	415202.0
58	391056.0
59	356519.5
60	321983.0
61	280970.5
62	239958.0
63	206716.0
64	173474.0
65	145405.5
66	117337.0
67	98556.0
68	79775.0
69	64423.0
70	49071.0
71	38600.0
72	28129.0
73	23567.5
74	19006.0
75	15525.0
76	12044.0
77	9769.0
78	7494.0
79	6011.5
80	4529.0
81	3497.0
82	2465.0
83	1794.5
84	1124.0
85	848.5
86	573.0
87	380.0
88	187.0
89	131.5
90	76.0
91	59.5
92	43.0
93	37.5
94	32.0
95	33.5
96	35.0
97	35.0
98	35.0
99	24.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03108795851738045
2	0.0048183763614913985
3	2.4006145573266758E-4
4	0.0
5	1.7147246838047683E-5
6	1.8861971521852452E-4
7	0.0
8	0.0
9	0.0
10	5.144174051414305E-5
11	0.0
12	1.7147246838047683E-5
13	0.0
14	0.0
15	0.0
16	5.315646519794781E-4
17	0.0
18	2.2291420889461986E-4
19	0.0
20	3.6009218359900135E-4
21	1.7147246838047683E-5
22	0.0
23	3.4294493676095365E-5
24	0.0010631293039589562
25	2.057669620565722E-4
26	6.173008861697166E-4
27	2.743559494087629E-4
28	0.0013889269938818622
29	3.943866772750967E-4
30	0.0011488655381491947
31	3.4294493676095367E-4
32	9.259513292545749E-4
33	3.25797689922906E-4
34	8.402150950643365E-4
35	2.057669620565722E-4
36	1.7147246838047683E-4
37	0.0018176081648330542
38	1.7147246838047683E-5
39	7.201843671980027E-4
40	6.858898735219073E-5
41	0.001080276550797004
42	0.004972701583033828
43	0.0044068424373782545
44	0.0042010754753216825
45	0.0023491728168125326
46	0.0026235287662212956
47	0.0013203380065296717
48	0.004183928228483635
49	0.003618069082828061
50	0.0016975774369667209
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5831840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.498891260978446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96913478244784	31.380191638303607
2	15.600123227661328	14.195766207864539
3	5.792278338423574	7.906267268197653
4	2.8413424955438615	5.171117329597892
5	1.6342430634908878	3.7178123719890097
6	1.0679027113995931	2.9153033605964485
7	0.7401550625674314	2.357336429561278
8	0.5232364935228527	1.9045344258057544
9	0.38404623753197314	1.5726310200589635
>10	2.239762201648337	18.39561973427946
>50	0.139139162803619	4.3589566765864864
>100	0.06590424474509696	5.201437708362974
>500	0.002352540910056627	0.6952967131905076
>1k	3.794373037300483E-4	0.22772911560538403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4294493676095365E-5
2	0.0	0.0	0.0	0.0	3.4294493676095365E-5
3	0.0	0.0	0.0	0.0	3.4294493676095365E-5
4	0.0	0.0	0.0	0.0	3.4294493676095365E-5
5	0.0	0.0	0.0	0.0	5.144174051414305E-5
6	0.0	0.0	0.0	0.0	5.144174051414305E-5
7	0.0	0.0	0.0	0.0	5.144174051414305E-5
8	0.0	0.0	0.0	0.0	5.144174051414305E-5
9	0.0	0.0	0.0	0.0	5.144174051414305E-5
10	0.0	0.0	0.0	1.7147246838047683E-5	5.144174051414305E-5
11	0.0	0.0	0.0	1.7147246838047683E-5	5.144174051414305E-5
12	0.0	0.0	0.0	1.7147246838047683E-5	2.4006145573266755E-4
13	0.0	0.0	0.0	1.7147246838047683E-5	3.25797689922906E-4
14	0.0	0.0	0.0	8.573623419023842E-5	3.25797689922906E-4
15	0.0	0.0	0.0	1.7147246838047683E-4	4.115339241131444E-4
16	1.7147246838047683E-5	0.0	0.0	3.6009218359900135E-4	4.458284177892398E-4
17	1.7147246838047683E-5	0.0	0.0	4.972701583033828E-4	4.6297566462728747E-4
18	1.7147246838047683E-5	0.0	0.0	6.344481330077642E-4	4.6297566462728747E-4
19	1.7147246838047683E-5	0.0	0.0	9.088040824165272E-4	4.6297566462728747E-4
20	1.7147246838047683E-5	0.0	0.0	0.0011145710444730994	5.658591456555736E-4
21	1.7147246838047683E-5	0.0	0.0	0.0014575159812340532	6.00153639331669E-4
22	1.7147246838047683E-5	0.0	0.0	0.0016975774369667206	6.00153639331669E-4
23	1.7147246838047683E-5	0.0	0.0	0.0022462893357842466	6.00153639331669E-4
24	1.7147246838047683E-5	0.0	0.0	0.003137946171362726	6.173008861697166E-4
25	1.7147246838047683E-5	0.0	0.0	0.004166780981645587	6.173008861697166E-4
26	1.7147246838047683E-5	0.0	0.0	0.0050927323109001615	6.858898735219073E-4
27	1.7147246838047683E-5	0.0	0.0	0.005778622184422069	7.373316140360503E-4
28	1.7147246838047683E-5	0.0	0.0	0.00680745699470493	7.373316140360503E-4
29	1.7147246838047683E-5	0.0	0.0	0.007939175286016077	7.373316140360503E-4
30	1.7147246838047683E-5	0.0	0.0	0.00961960547614475	7.373316140360503E-4
31	1.7147246838047683E-5	0.0	0.0	0.013083349337430382	7.373316140360503E-4
32	3.4294493676095365E-5	0.0	0.0	0.018416143104063212	7.373316140360503E-4
33	3.4294493676095365E-5	0.0	0.0	0.023063046997174135	7.544788608740981E-4
34	3.4294493676095365E-5	0.0	0.0	0.02906458339049082	7.887733545501934E-4
35	3.4294493676095365E-5	0.0	0.0	0.035031825290131414	7.887733545501934E-4
36	3.4294493676095365E-5	0.0	0.0	0.04298814782298554	7.887733545501934E-4
37	3.4294493676095365E-5	0.0	0.0	0.05456253943866773	8.059206013882411E-4
38	3.4294493676095365E-5	0.0	0.0	0.06989217811188235	8.230678482262888E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2380	0.0	28.388002	1
GTATCAA	6300	0.0	27.806475	1
TCAACGC	9245	0.0	18.608255	4
TATCAAC	9300	0.0	18.546312	2
GTATAGG	1045	0.0	18.322147	1
ATCAACG	9500	0.0	18.155085	3
CAACGCA	9530	0.0	18.051767	5
AACGCAG	9965	0.0	17.352062	6
TATACCG	275	0.0	15.19941	5
ATAGGGG	935	0.0	14.822954	3
TATAGGG	1110	0.0	14.666726	2
TAGGGCG	685	0.0	14.452366	31
CGCGTAA	320	0.0	14.43694	10
ACGCAGA	12105	0.0	14.284452	7
TACGCGG	110	0.0010191184	13.999457	10
AGAGTAC	12580	0.0	13.780068	11
ATACCGT	520	0.0	13.537938	6
GTACTAG	440	0.0	13.504686	1
ACCGTAT	315	1.8189894E-12	13.269326	8
GTCTTAG	925	0.0	13.085622	1
>>END_MODULE