FastQCFastQC Report
Thu 2 Feb 2017
SRR4061960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622952
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33630.1282143173035572No Hit
GCGCAAGACGGACCAGAGCGAAAGC26510.10106932951880172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.014.4023331
ATTACGC400.00528316414.2488683
CGAATTG500.00149962813.30046814
GCGTTAT2100.012.6593761
GTATCAA28950.012.5281911
ATACGCT2150.012.373718
ACGGTCC701.0901202E-412.2158818
TCGCGTA2450.012.0224389
CGTTATT2250.011.8183512
ACGAACG1302.6193447E-911.69227315
GTCCTAA3100.011.6384591
TACGCTA2300.011.5671719
ATATACG2550.011.5505296
CGCCGGT3500.011.4017087
ACGGCGT752.0723336E-411.4014898
AGAGCGA5850.011.20509415
TAAGGCG600.005875942311.0837235
ACGCTAT2500.011.0216510
CGATAAC1303.2619937E-810.9631810
CGAACGA1303.270361E-810.96129616
Produced by FastQC (version 0.11.5)