##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061960_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2622952
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236313893658746	32.0	32.0	32.0	32.0	32.0
2	30.746331995400602	32.0	32.0	32.0	32.0	32.0
3	30.77955219920151	32.0	32.0	32.0	32.0	32.0
4	30.791588637535114	32.0	32.0	32.0	32.0	32.0
5	30.695861380612378	32.0	32.0	32.0	32.0	32.0
6	34.3605517752517	36.0	36.0	36.0	32.0	36.0
7	34.29741642241261	36.0	36.0	36.0	32.0	36.0
8	34.246429976606514	36.0	36.0	36.0	32.0	36.0
9	34.38880886878601	36.0	36.0	36.0	32.0	36.0
10	34.05788516145168	36.0	36.0	36.0	32.0	36.0
11	34.36982491482879	36.0	36.0	36.0	32.0	36.0
12	34.1866862222412	36.0	36.0	36.0	32.0	36.0
13	34.283748234813295	36.0	36.0	36.0	32.0	36.0
14	34.1869611033675	36.0	36.0	36.0	32.0	36.0
15	34.124067081669814	36.0	36.0	36.0	32.0	36.0
16	34.135618570221645	36.0	36.0	36.0	32.0	36.0
17	34.066279138924386	36.0	36.0	36.0	32.0	36.0
18	34.06516817692432	36.0	36.0	36.0	32.0	36.0
19	34.07856186464716	36.0	36.0	36.0	32.0	36.0
20	34.06375869630859	36.0	36.0	36.0	32.0	36.0
21	34.04612512924369	36.0	36.0	36.0	32.0	36.0
22	34.017980504408776	36.0	36.0	36.0	32.0	36.0
23	33.98113232724045	36.0	36.0	36.0	32.0	36.0
24	33.95953833695775	36.0	36.0	36.0	32.0	36.0
25	33.598453193196065	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	35.0
5	196.0
6	556.0
7	125.0
8	468.0
9	340.0
10	193.0
11	65.0
12	114.0
13	115.0
14	339.0
15	533.0
16	810.0
17	1260.0
18	1750.0
19	2353.0
20	3737.0
21	5380.0
22	8050.0
23	11998.0
24	16904.0
25	23899.0
26	32197.0
27	40995.0
28	54294.0
29	70625.0
30	91428.0
31	123437.0
32	174186.0
33	244565.0
34	533692.0
35	1178308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78672965002678	16.8340567650877	11.434987409046805	25.944226175838715
2	17.055409091688922	18.889950037076645	37.25232515359643	26.802315717638
3	19.799857640282845	22.192408269673685	27.677069906093678	30.3306641839498
4	13.134442403696681	15.171254409139975	34.37602114175707	37.31828204540628
5	15.34682937377696	35.98687745823597	32.960088907432464	15.70620426055461
6	35.803844401903504	34.09627256447838	16.182066222962494	13.917816810655614
7	31.649305548929497	29.479143568231613	19.44119502604263	19.430355856796254
8	28.663290437745676	33.027655994104	18.44346152392882	19.8655920442215
9	26.97068304441344	14.63007884144491	18.155785809303328	40.243452304838314
10	16.727221223478193	26.377099191761783	30.473341042474978	26.42233854228505
11	37.53187505703053	21.150567861848472	21.510867203902244	19.80668987721875
12	24.820626176323497	23.1842909990494	28.1287628035535	23.86632002107361
13	29.769862856815642	19.17061299281358	24.713602656970252	26.34592149340052
14	23.745492590614816	19.15046378556354	24.493168128135434	32.61087549568621
15	25.522787323531244	26.889602969871035	21.491593598926155	26.09601610767157
16	26.216743093438648	25.57167390853752	23.081903059068548	25.129679938955285
17	24.49351974005668	25.873781515684552	24.443360329326687	25.189338414932084
18	25.170335724833805	25.0375480968697	25.22487827164631	24.56723790665018
19	25.88258056834418	24.83147508151477	24.623719271679263	24.662225078461784
20	26.072803857900023	24.246811573242134	24.406705150519386	25.273679418338457
21	26.736186636199687	24.00262999028445	24.152484486033803	25.108698887482056
22	26.32154630936415	24.029301289278102	24.41587099738725	25.233281403970498
23	24.942440843992365	24.14462553543179	24.952857582202565	25.96007603837328
24	25.018307567904003	24.617010258725337	24.869213940387734	25.495468232982933
25	25.204877996660286	24.35983734923208	24.716628049320306	25.71865660478733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	358.0
1	358.0
2	550.0
3	742.0
4	742.0
5	742.0
6	1408.5
7	2075.0
8	2075.0
9	2075.0
10	2578.5
11	3082.0
12	3082.0
13	3082.0
14	4130.5
15	5179.0
16	5179.0
17	5179.0
18	8806.0
19	12433.0
20	12433.0
21	12433.0
22	20335.5
23	28238.0
24	28238.0
25	28238.0
26	42924.0
27	57610.0
28	57610.0
29	57610.0
30	74925.0
31	92240.0
32	92240.0
33	92240.0
34	118055.5
35	143871.0
36	143871.0
37	143871.0
38	173005.5
39	202140.0
40	202140.0
41	202140.0
42	235323.5
43	268507.0
44	268507.0
45	268507.0
46	303035.5
47	337564.0
48	337564.0
49	337564.0
50	353923.0
51	370282.0
52	370282.0
53	370282.0
54	351624.0
55	332966.0
56	332966.0
57	332966.0
58	309400.5
59	285835.0
60	285835.0
61	285835.0
62	249493.5
63	213152.0
64	213152.0
65	213152.0
66	173615.0
67	134078.0
68	134078.0
69	134078.0
70	101186.0
71	68294.0
72	68294.0
73	68294.0
74	50852.0
75	33410.0
76	33410.0
77	33410.0
78	25891.5
79	18373.0
80	18373.0
81	18373.0
82	12623.5
83	6874.0
84	6874.0
85	6874.0
86	5050.0
87	3226.0
88	3226.0
89	3226.0
90	2183.5
91	1141.0
92	1141.0
93	1141.0
94	710.0
95	279.0
96	279.0
97	279.0
98	641.0
99	1003.0
100	1003.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014106243652190356
2	2.66874879906304E-4
3	7.243746740313966E-4
4	0.010484370282033374
5	0.03427435957653819
6	0.06427872107457552
7	0.10770307653361556
8	0.1093805757787409
9	0.11959807118086796
10	0.13538181407818367
11	0.14262556081849764
12	0.13580118888946502
13	0.1306543162055577
14	0.11814932183280519
15	0.13644931359780887
16	0.12325806953386871
17	0.1259649433157755
18	0.11357432389155425
19	0.09790495594276982
20	0.09374933281280023
21	0.0918812086534561
22	0.10392870323208356
23	0.08273121277095426
24	0.09344433295005017
25	0.09027995937401828
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2622952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.17278160215675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30843195600308	36.04779514169108
2	14.80034045733486	14.555478178921835
3	5.079154571106972	7.492684753459178
4	2.23721293998807	4.400399331822094
5	1.1522361228515463	2.8329327611547472
6	0.753004309530479	2.221638987481505
7	0.4734667752416484	1.6297174834384507
8	0.34218748872611715	1.3461048520095866
9	0.25656865838276316	1.1354575144152579
>10	1.4174807284141189	12.805140393688792
>50	0.10101911147088904	3.40675404841254
>100	0.0669291051014262	6.543277534777295
>500	0.00808213433895975	2.690693205194949
>1k	0.0038856415091082304	2.891925813532667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3363	0.1282143173035572	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2651	0.10106932951880172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.812498284375772E-5	1.1437494853127317E-4
2	0.0	0.0	0.0	3.812498284375772E-5	1.1437494853127317E-4
3	0.0	0.0	0.0	3.812498284375772E-5	1.1437494853127317E-4
4	0.0	0.0	0.0	3.812498284375772E-5	1.1437494853127317E-4
5	0.0	0.0	0.0	3.812498284375772E-5	1.5249993137503087E-4
6	0.0	0.0	0.0	3.812498284375772E-5	1.9062491421878861E-4
7	0.0	0.0	0.0	3.812498284375772E-5	1.9062491421878861E-4
8	0.0	0.0	0.0	3.812498284375772E-5	1.9062491421878861E-4
9	0.0	0.0	0.0	7.624996568751543E-5	1.9062491421878861E-4
10	0.0	0.0	0.0	7.624996568751543E-5	1.9062491421878861E-4
11	0.0	0.0	0.0	1.1437494853127317E-4	1.9062491421878861E-4
12	0.0	0.0	0.0	1.1437494853127317E-4	5.33749759812608E-4
13	0.0	0.0	0.0	1.1437494853127317E-4	6.862496911876389E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1035	0.0	14.402333	1
ATTACGC	40	0.005283164	14.248868	3
CGAATTG	50	0.001499628	13.300468	14
GCGTTAT	210	0.0	12.659376	1
GTATCAA	2895	0.0	12.528191	1
ATACGCT	215	0.0	12.37371	8
ACGGTCC	70	1.0901202E-4	12.215881	8
TCGCGTA	245	0.0	12.022438	9
CGTTATT	225	0.0	11.818351	2
ACGAACG	130	2.6193447E-9	11.692273	15
GTCCTAA	310	0.0	11.638459	1
TACGCTA	230	0.0	11.567171	9
ATATACG	255	0.0	11.550529	6
CGCCGGT	350	0.0	11.401708	7
ACGGCGT	75	2.0723336E-4	11.401489	8
AGAGCGA	585	0.0	11.205094	15
TAAGGCG	60	0.0058759423	11.083723	5
ACGCTAT	250	0.0	11.02165	10
CGATAAC	130	3.2619937E-8	10.96318	10
CGAACGA	130	3.270361E-8	10.961296	16
>>END_MODULE