FastQCFastQC Report
Thu 2 Feb 2017
SRR4061960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622952
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26690.10175557920998936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30600.026.2508471
GGTATCA14450.025.891251
TCAACGC43500.018.206214
ATCAACG44750.017.7468193
CGCAATA6100.017.67146536
GATATAC3500.017.6060491
TCTAGCG6300.017.46032528
CAACGCA45400.017.4442755
TAGCGGC6550.017.12912630
AACGCAG46550.017.0605836
TATCAAC47250.016.9015942
GTCCTAA4050.016.8452931
TACGAAT6450.016.7144641
TAGGACG3050.016.5895394
ATACGAA6400.016.5009540
CGGATCG1206.412258E-616.50000826
CGAGCCG8300.016.43342815
CTAGCGG6800.016.17586129
CGAATGC6600.016.00153243
CAATACG6600.016.00092138
Produced by FastQC (version 0.11.5)