##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4061960_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2622952 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.302320057705973 32.0 32.0 32.0 32.0 32.0 2 31.457347294193717 32.0 32.0 32.0 32.0 32.0 3 31.577262946481675 32.0 32.0 32.0 32.0 32.0 4 31.656298704665584 32.0 32.0 32.0 32.0 32.0 5 31.62925017308742 32.0 32.0 32.0 32.0 32.0 6 35.28247181038768 36.0 36.0 36.0 36.0 36.0 7 35.282544247855085 36.0 36.0 36.0 36.0 36.0 8 35.231054171025626 36.0 36.0 36.0 36.0 36.0 9 35.31406598367031 36.0 36.0 36.0 36.0 36.0 10 35.20075586590986 36.0 36.0 36.0 36.0 36.0 11 35.31811638184763 36.0 36.0 36.0 36.0 36.0 12 35.24429459631743 36.0 36.0 36.0 36.0 36.0 13 35.27678280044774 36.0 36.0 36.0 36.0 36.0 14 35.24117940396927 36.0 36.0 36.0 36.0 36.0 15 35.21950725747173 36.0 36.0 36.0 36.0 36.0 16 35.22934235929594 36.0 36.0 36.0 36.0 36.0 17 35.210059505473225 36.0 36.0 36.0 36.0 36.0 18 35.20742659415803 36.0 36.0 36.0 36.0 36.0 19 35.19447782498498 36.0 36.0 36.0 36.0 36.0 20 35.20055647224959 36.0 36.0 36.0 36.0 36.0 21 35.190780464148794 36.0 36.0 36.0 36.0 36.0 22 35.17489797754591 36.0 36.0 36.0 36.0 36.0 23 35.14526952837871 36.0 36.0 36.0 36.0 36.0 24 35.127502523873865 36.0 36.0 36.0 36.0 36.0 25 35.114612848424215 36.0 36.0 36.0 36.0 36.0 26 35.068502969173664 36.0 36.0 36.0 36.0 36.0 27 35.05844864869811 36.0 36.0 36.0 36.0 36.0 28 35.03675705845932 36.0 36.0 36.0 36.0 36.0 29 35.02025008463746 36.0 36.0 36.0 36.0 36.0 30 35.00112735574269 36.0 36.0 36.0 36.0 36.0 31 35.00307325486703 36.0 36.0 36.0 36.0 36.0 32 34.97858557838649 36.0 36.0 36.0 36.0 36.0 33 34.96064091146159 36.0 36.0 36.0 36.0 36.0 34 34.954932457780394 36.0 36.0 36.0 36.0 36.0 35 34.93703125333594 36.0 36.0 36.0 36.0 36.0 36 34.9154609005426 36.0 36.0 36.0 36.0 36.0 37 34.90358496838677 36.0 36.0 36.0 32.0 36.0 38 34.88850386892326 36.0 36.0 36.0 32.0 36.0 39 34.87585247461639 36.0 36.0 36.0 32.0 36.0 40 34.86409892365548 36.0 36.0 36.0 32.0 36.0 41 34.835048449228196 36.0 36.0 36.0 32.0 36.0 42 34.807266392980125 36.0 36.0 36.0 32.0 36.0 43 34.806682318242956 36.0 36.0 36.0 32.0 36.0 44 34.76161515727318 36.0 36.0 36.0 32.0 36.0 45 34.752148342783244 36.0 36.0 36.0 32.0 36.0 46 34.72776093500758 36.0 36.0 36.0 32.0 36.0 47 34.70891994973602 36.0 36.0 36.0 32.0 36.0 48 34.68405331092601 36.0 36.0 36.0 32.0 36.0 49 34.67611683324743 36.0 36.0 36.0 32.0 36.0 50 34.26904952892771 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 5.0 20 24.0 21 57.0 22 176.0 23 457.0 24 1181.0 25 2827.0 26 5995.0 27 11336.0 28 19695.0 29 31381.0 30 47371.0 31 69716.0 32 106278.0 33 181763.0 34 419372.0 35 1725318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59117362154576 17.280999488967026 11.793191820421487 26.334635069065726 2 16.50682696135023 19.32091827319741 36.96445374396264 27.207801021489715 3 19.18976415777715 22.574477494719666 27.892365056005858 30.34339329149733 4 12.899745363142506 15.570401429534902 34.482077629357164 37.04777557796543 5 15.031765735705418 36.27134617789422 32.77539962607017 15.921488460330194 6 35.971297900374545 34.1493375382776 16.020929154415803 13.858435406932056 7 31.677056995324353 29.469048613928123 19.452471871387658 19.401422519359866 8 28.714898328295753 33.109984475506984 18.287334270699578 19.88778292549768 9 27.125582168488023 14.354742290365968 18.08279373774282 40.43688180340319 10 16.71952697553252 26.365227562390604 30.46042415584584 26.454821306231036 11 37.74396176521721 20.98299930765031 21.47576471090588 19.7972742162266 12 24.901942544125856 23.05497775026001 28.13353046491129 23.90954924070284 13 29.897115921297836 19.024518938966477 24.751196361961636 26.32716877777405 14 23.836730523471264 18.965539590507184 24.511504594822934 32.68622529119862 15 25.543357255489234 26.796258566683644 21.546486554081053 26.11389762374607 16 26.346823458184755 25.516416558422623 23.002130059845268 25.134629923547358 17 24.60521580265289 25.763147781583502 24.470138988437455 25.161497427326157 18 25.282601650813014 24.82967650927391 25.354924798413997 24.532797041499073 19 25.986483931082233 24.61600517279767 24.700261384882378 24.69724951123772 20 26.21479002784277 24.12416129515586 24.38436519588874 25.276683481112627 21 26.832678155253376 23.907194606380372 24.239301458548024 25.020825779818228 22 26.33246815038933 23.903296743516467 24.54852395316422 25.21571115292998 23 25.054728454602643 23.967593739873045 24.983587182370997 25.99409062315332 24 25.227474618079782 24.49443942461293 24.892810711685023 25.385275245622264 25 25.4008944900526 24.109941985103013 24.824901151262303 25.66426237358208 26 25.047799470593464 24.90627872495603 25.09259658161824 24.95332522283227 27 25.568923093111618 24.45730703508813 24.673743717922424 25.300026153877823 28 25.32408759458035 24.303309845354494 24.971234152095654 25.401368407969493 29 25.021798116840955 24.531087908078476 25.065260912116322 25.38185306296425 30 25.076698840719946 24.686790032944188 25.03861153271486 25.197899593621003 31 25.62375592893626 24.456059057371096 24.40756387581726 25.512621137875385 32 25.621137903899765 24.446992063830955 24.25655603203748 25.675314000231804 33 25.04458726942911 24.272745226169352 24.791818054965674 25.890849449435866 34 25.68321469379103 24.405178786632206 24.895965620140217 25.015640899436548 35 25.898580335142622 24.212154057484284 25.04114652891809 24.848119078455003 36 24.98224325539174 24.81045175522037 24.643654637322065 25.563650352065824 37 25.90572078122361 24.3491296502245 24.612348646217885 25.132800922334003 38 25.25471301037914 24.265255330635103 24.825425703558434 25.654605955427318 39 25.828540971831437 24.301009102818078 24.289266482749785 25.581183442600704 40 25.9826531195791 24.477553899235595 24.599363312301033 24.94042966888427 41 25.05411894960056 24.80470283950062 25.06467967453142 25.076498536367403 42 26.098570316354397 24.73907835007995 24.596254867855873 24.566096465709784 43 25.678440355429622 23.951771619769914 24.89288385857543 25.476904166225033 44 25.161914122891933 24.659062444120707 24.53335905607698 25.645664376910382 45 25.244806114777123 24.84185395357491 24.605473652746664 25.307866278901304 46 25.331236922851687 24.518242431266813 24.60261481964973 25.54790582623177 47 25.5000922637248 24.521258019700213 24.837432841920975 25.14121687465401 48 25.986909704257833 24.876670838958557 24.018599551100536 25.117819905683074 49 25.3783183757254 24.953400051928107 24.1837431472627 25.48453842508379 50 25.451626419368722 25.085696835078913 24.251737288636395 25.210939456915966 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 83.0 2 49.0 3 146.5 4 244.0 5 261.5 6 279.0 7 234.5 8 190.0 9 271.5 10 353.0 11 602.5 12 852.0 13 1575.0 14 2298.0 15 3005.5 16 3713.0 17 3587.0 18 3461.0 19 3345.5 20 3230.0 21 3809.0 22 4388.0 23 5510.5 24 6633.0 25 8717.0 26 10801.0 27 13995.0 28 17189.0 29 21024.5 30 24860.0 31 29482.0 32 34104.0 33 39948.0 34 45792.0 35 54225.5 36 62659.0 37 73358.5 38 84058.0 39 94032.0 40 104006.0 41 114463.5 42 124921.0 43 131858.5 44 138796.0 45 153183.5 46 167571.0 47 179832.0 48 192093.0 49 201462.5 50 210832.0 51 209908.5 52 208985.0 53 209520.0 54 210055.0 55 209394.0 56 208733.0 57 200645.0 58 192557.0 59 178368.0 60 164179.0 61 143854.0 62 123529.0 63 106928.0 64 90327.0 65 75493.5 66 60660.0 67 51887.5 68 43115.0 69 37249.5 70 31384.0 71 24290.0 72 17196.0 73 14690.0 74 12184.0 75 8963.0 76 5742.0 77 5010.0 78 4278.0 79 3544.5 80 2811.0 81 2213.5 82 1616.0 83 1367.5 84 1119.0 85 858.5 86 598.0 87 409.0 88 220.0 89 144.0 90 68.0 91 52.5 92 37.0 93 29.5 94 22.0 95 21.5 96 21.0 97 18.5 98 16.0 99 13.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030957486069131266 2 0.004422498009875896 3 4.574997941250926E-4 4 3.812498284375772E-5 5 0.0 6 3.0499986275006174E-4 7 0.0 8 0.0 9 0.0 10 3.812498284375772E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 8.006246397189122E-4 17 0.0 18 7.624996568751543E-5 19 0.0 20 3.431248455938195E-4 21 3.812498284375772E-5 22 0.0 23 7.624996568751543E-5 24 8.387496225626699E-4 25 1.9062491421878861E-4 26 5.718747426563658E-4 27 5.33749759812608E-4 28 0.0019062491421878859 29 7.243746740313966E-4 30 0.0011818744681564893 31 4.1937481128133494E-4 32 9.149995882501852E-4 33 2.287498970625463E-4 34 8.006246397189122E-4 35 3.431248455938195E-4 36 1.9062491421878861E-4 37 0.0015631242965940665 38 0.0 39 0.001067499519625216 40 7.624996568751543E-5 41 9.912495539377009E-4 42 0.005108747701063535 43 0.0037362483186882565 44 0.004231873095657107 45 0.002287498970625463 46 0.002134999039250432 47 0.001372499382375278 48 0.003545623404469468 49 0.0036218733701569835 50 0.0016393742622815817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2622952.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.985740854812725 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45289305725102 36.216115365412094 2 15.407296500473771 15.4029026029189 3 5.273285609770501 7.907672638302042 4 2.309942181385385 4.618566850733227 5 1.1869732029950426 2.966586746325861 6 0.73085621441103 2.191943360140752 7 0.49341980558017834 1.726476817405401 8 0.32463120357473196 1.2981544972218007 9 0.2688671420887508 1.209557095994017 >10 1.3887720343528664 12.581550674912952 >50 0.09282288222192428 3.1847590885636774 >100 0.0598800282327678 5.851237753796297 >500 0.007290467243729733 2.5068602762876377 >1k 0.0030696704184068713 2.3376162319854257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2669 0.10175557920998936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.624996568751543E-5 2 0.0 0.0 0.0 0.0 7.624996568751543E-5 3 0.0 0.0 0.0 0.0 7.624996568751543E-5 4 0.0 0.0 0.0 0.0 7.624996568751543E-5 5 0.0 0.0 0.0 0.0 1.1437494853127317E-4 6 0.0 0.0 0.0 0.0 1.9062491421878861E-4 7 0.0 0.0 0.0 0.0 1.9062491421878861E-4 8 0.0 0.0 0.0 0.0 1.9062491421878861E-4 9 0.0 0.0 0.0 0.0 1.9062491421878861E-4 10 0.0 0.0 0.0 1.5249993137503087E-4 1.9062491421878861E-4 11 0.0 0.0 0.0 1.5249993137503087E-4 1.9062491421878861E-4 12 0.0 0.0 0.0 1.5249993137503087E-4 4.1937481128133494E-4 13 0.0 0.0 0.0 1.5249993137503087E-4 5.718747426563658E-4 14 0.0 0.0 0.0 1.5249993137503087E-4 5.718747426563658E-4 15 0.0 0.0 0.0 1.5249993137503087E-4 7.624996568751545E-4 16 0.0 0.0 0.0 2.2874989706254633E-4 7.624996568751545E-4 17 0.0 0.0 0.0 2.66874879906304E-4 7.624996568751545E-4 18 0.0 0.0 0.0 3.0499986275006174E-4 8.006246397189122E-4 19 0.0 0.0 0.0 3.4312484559381946E-4 8.006246397189122E-4 20 0.0 0.0 0.0 3.8124982843757723E-4 8.006246397189122E-4 21 0.0 0.0 0.0 4.1937481128133494E-4 9.149995882501853E-4 22 0.0 0.0 0.0 4.5749979412509266E-4 9.53124571093943E-4 23 0.0 0.0 0.0 6.099997255001235E-4 9.53124571093943E-4 24 0.0 0.0 0.0 8.768746054064276E-4 9.53124571093943E-4 25 0.0 0.0 0.0 9.912495539377007E-4 9.53124571093943E-4 26 0.0 0.0 0.0 0.0010293745367814585 0.0011437494853127315 27 0.0 0.0 0.0 0.001105624502468974 0.0012581244338440048 28 0.0 0.0 0.0 0.001219999451000247 0.0012581244338440048 29 0.0 0.0 0.0 0.0017918741936566128 0.0012581244338440048 30 0.0 0.0 0.0 0.0022493739877817056 0.0012581244338440048 31 0.0 0.0 0.0 0.003278748524563164 0.0012581244338440048 32 0.0 0.0 0.0 0.004384373027032138 0.0014106243652190356 33 0.0 0.0 0.0 0.006252497186376266 0.0014106243652190356 34 0.0 0.0 0.0 0.00785374646581409 0.0014487493480627935 35 0.0 0.0 0.0 0.010255620384970826 0.001486874330906551 36 0.0 0.0 0.0 0.012848119218346351 0.001486874330906551 37 0.0 0.0 0.0 0.018338116747847463 0.001486874330906551 38 0.0 0.0 0.0 0.02420936410578615 0.001486874330906551 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3060 0.0 26.250847 1 GGTATCA 1445 0.0 25.89125 1 TCAACGC 4350 0.0 18.20621 4 ATCAACG 4475 0.0 17.746819 3 CGCAATA 610 0.0 17.671465 36 GATATAC 350 0.0 17.606049 1 TCTAGCG 630 0.0 17.460325 28 CAACGCA 4540 0.0 17.444275 5 TAGCGGC 655 0.0 17.129126 30 AACGCAG 4655 0.0 17.060583 6 TATCAAC 4725 0.0 16.901594 2 GTCCTAA 405 0.0 16.845293 1 TACGAAT 645 0.0 16.71446 41 TAGGACG 305 0.0 16.589539 4 ATACGAA 640 0.0 16.50095 40 CGGATCG 120 6.412258E-6 16.500008 26 CGAGCCG 830 0.0 16.433428 15 CTAGCGG 680 0.0 16.175861 29 CGAATGC 660 0.0 16.001532 43 CAATACG 660 0.0 16.000921 38 >>END_MODULE