##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061959_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4414
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.411644766651563	32.0	32.0	32.0	32.0	32.0
2	31.167195287720887	32.0	32.0	32.0	32.0	32.0
3	31.108744902582693	32.0	32.0	32.0	32.0	32.0
4	31.072043497961033	32.0	32.0	32.0	32.0	32.0
5	31.16107838695061	32.0	32.0	32.0	32.0	32.0
6	34.784775713638425	36.0	36.0	36.0	36.0	36.0
7	34.72927050294518	36.0	36.0	36.0	36.0	36.0
8	34.79859537834164	36.0	36.0	36.0	36.0	36.0
9	34.72927050294518	36.0	36.0	36.0	32.0	36.0
10	34.64454009968283	36.0	36.0	36.0	32.0	36.0
11	34.82442229270503	36.0	36.0	36.0	36.0	36.0
12	34.63094698685999	36.0	36.0	36.0	32.0	36.0
13	34.63797009515179	36.0	36.0	36.0	32.0	36.0
14	34.752378794744	36.0	36.0	36.0	32.0	36.0
15	34.649977344811965	36.0	36.0	36.0	32.0	36.0
16	34.58087902129588	36.0	36.0	36.0	32.0	36.0
17	34.54599003171727	36.0	36.0	36.0	32.0	36.0
18	34.45582238332578	36.0	36.0	36.0	32.0	36.0
19	34.545763479836886	36.0	36.0	36.0	32.0	36.0
20	34.51880380607159	36.0	36.0	36.0	32.0	36.0
21	34.51223380154055	36.0	36.0	36.0	32.0	36.0
22	34.42433167195288	36.0	36.0	36.0	32.0	36.0
23	34.43248753964658	36.0	36.0	36.0	32.0	36.0
24	34.32283642954236	36.0	36.0	36.0	32.0	36.0
25	34.13140009062075	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	3.0
17	2.0
18	5.0
19	2.0
20	4.0
21	8.0
22	8.0
23	13.0
24	18.0
25	32.0
26	39.0
27	29.0
28	61.0
29	92.0
30	105.0
31	175.0
32	233.0
33	322.0
34	844.0
35	2415.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.40734028092433	19.25690983235161	10.512007249660172	26.823742637063887
2	17.308563661078388	20.84277299501586	36.429542365201634	25.419120978704125
3	19.98187584956955	21.83960126869053	29.519710013593116	28.658812868146804
4	14.208021753908906	16.655336505778383	36.18853387718106	32.94810786313165
5	14.68721668177697	34.72348141432457	34.13417951042611	16.455122393472347
6	31.738834731353432	36.091589208796194	17.66039446837452	14.509181591475857
7	28.532547062826037	30.006804264005442	22.317985937854388	19.142662735314133
8	26.961451247165535	32.04081632653061	20.58956916099773	20.408163265306122
9	25.2665003402132	16.284871853027898	20.503515536402812	37.94511227035609
10	16.62508505330007	27.14901338171921	30.868677704694942	25.357223860285778
11	33.26525981393238	22.05582028590878	23.712275924665306	20.966643975493533
12	22.82214156079855	24.36479128856624	29.174228675136117	23.638838475499092
13	28.77240753346948	21.238938053097346	26.59405491263898	23.394599500794193
14	22.29530505783624	19.52823769562259	26.491267861193013	31.68518938534815
15	24.41016333938294	27.041742286751365	23.11705989110708	25.43103448275862
16	24.676797459741437	27.08097074166478	23.542753458834202	24.69947833975958
17	23.560090702947846	25.396825396825395	25.736961451247165	25.30612244897959
18	23.764172335600907	26.780045351473923	26.077097505668934	23.378684807256235
19	24.121514395828612	25.300385400136022	26.365903423260033	24.212196780775336
20	24.17233560090703	27.573696145124714	25.238095238095237	23.015873015873016
21	24.693877551020407	26.258503401360546	24.671201814058957	24.37641723356009
22	24.9546279491833	26.066243194192378	26.383847549909255	22.595281306715066
23	24.784580498866212	26.281179138321995	24.85260770975057	24.081632653061224
24	23.980054397098822	25.54397098821396	25.97461468721668	24.501359927470535
25	24.648526077097504	26.009070294784582	25.442176870748302	23.900226757369612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	3.5
7	5.0
8	5.0
9	5.0
10	7.0
11	9.0
12	9.0
13	9.0
14	16.0
15	23.0
16	23.0
17	23.0
18	38.0
19	53.0
20	53.0
21	53.0
22	77.0
23	101.0
24	101.0
25	101.0
26	144.0
27	187.0
28	187.0
29	187.0
30	226.0
31	265.0
32	265.0
33	265.0
34	304.5
35	344.0
36	344.0
37	344.0
38	375.0
39	406.0
40	406.0
41	406.0
42	430.5
43	455.0
44	455.0
45	455.0
46	455.5
47	456.0
48	456.0
49	456.0
50	477.5
51	499.0
52	499.0
53	499.0
54	464.0
55	429.0
56	429.0
57	429.0
58	407.0
59	385.0
60	385.0
61	385.0
62	342.0
63	299.0
64	299.0
65	299.0
66	253.0
67	207.0
68	207.0
69	207.0
70	176.5
71	146.0
72	146.0
73	146.0
74	112.5
75	79.0
76	79.0
77	79.0
78	58.0
79	37.0
80	37.0
81	37.0
82	27.0
83	17.0
84	17.0
85	17.0
86	11.5
87	6.0
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.022655188038060717
5	0.045310376076121435
6	0.06796556411418216
7	0.11327594019030357
8	0.09062075215224287
9	0.11327594019030357
10	0.11327594019030357
11	0.15858631626642503
12	0.1359311282283643
13	0.15858631626642503
14	0.11327594019030357
15	0.1359311282283643
16	0.11327594019030357
17	0.09062075215224287
18	0.09062075215224287
19	0.06796556411418216
20	0.09062075215224287
21	0.09062075215224287
22	0.1359311282283643
23	0.09062075215224287
24	0.045310376076121435
25	0.09062075215224287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4414.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.52695967376529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.50990099009901	83.75623017671046
2	7.797029702970297	14.27276846397825
3	0.6188118811881188	1.6991391028545535
4	0.07425742574257425	0.2718622564567286
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE