##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4414
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323742637063887	32.0	32.0	32.0	32.0	32.0
2	31.646579066606254	32.0	32.0	32.0	32.0	32.0
3	31.672632532850024	32.0	32.0	32.0	32.0	32.0
4	31.66787494336203	32.0	32.0	32.0	32.0	32.0
5	31.75668328047123	32.0	32.0	32.0	32.0	32.0
6	35.337109198006345	36.0	36.0	36.0	36.0	36.0
7	35.416175804259176	36.0	36.0	36.0	36.0	36.0
8	35.34594472134119	36.0	36.0	36.0	36.0	36.0
9	35.44834617127322	36.0	36.0	36.0	36.0	36.0
10	35.267557770729496	36.0	36.0	36.0	36.0	36.0
11	35.4322609877662	36.0	36.0	36.0	36.0	36.0
12	35.352061622111464	36.0	36.0	36.0	36.0	36.0
13	35.44064340734028	36.0	36.0	36.0	36.0	36.0
14	35.32374263706389	36.0	36.0	36.0	36.0	36.0
15	35.3808337109198	36.0	36.0	36.0	36.0	36.0
16	35.33235160851835	36.0	36.0	36.0	36.0	36.0
17	35.307884005437245	36.0	36.0	36.0	36.0	36.0
18	35.34685092886271	36.0	36.0	36.0	36.0	36.0
19	35.30924331671953	36.0	36.0	36.0	36.0	36.0
20	35.2816039873131	36.0	36.0	36.0	36.0	36.0
21	35.23878568192116	36.0	36.0	36.0	36.0	36.0
22	35.28341640235614	36.0	36.0	36.0	36.0	36.0
23	35.20299048482102	36.0	36.0	36.0	36.0	36.0
24	35.2102401449932	36.0	36.0	36.0	36.0	36.0
25	35.180788400543726	36.0	36.0	36.0	36.0	36.0
26	35.240144993203444	36.0	36.0	36.0	36.0	36.0
27	35.10353420933394	36.0	36.0	36.0	36.0	36.0
28	35.14635251472587	36.0	36.0	36.0	36.0	36.0
29	35.09220661531491	36.0	36.0	36.0	36.0	36.0
30	35.14272768463978	36.0	36.0	36.0	36.0	36.0
31	35.01495242410512	36.0	36.0	36.0	36.0	36.0
32	35.05437245129134	36.0	36.0	36.0	36.0	36.0
33	35.0373810602628	36.0	36.0	36.0	36.0	36.0
34	34.983235160851834	36.0	36.0	36.0	36.0	36.0
35	35.113049388309925	36.0	36.0	36.0	36.0	36.0
36	34.86905301314001	36.0	36.0	36.0	36.0	36.0
37	34.9474399637517	36.0	36.0	36.0	36.0	36.0
38	34.94358858178523	36.0	36.0	36.0	36.0	36.0
39	34.90869959220662	36.0	36.0	36.0	36.0	36.0
40	34.90507476212053	36.0	36.0	36.0	36.0	36.0
41	34.90643407340281	36.0	36.0	36.0	36.0	36.0
42	34.83982782057091	36.0	36.0	36.0	36.0	36.0
43	34.840960579972815	36.0	36.0	36.0	36.0	36.0
44	34.90348889895786	36.0	36.0	36.0	36.0	36.0
45	34.684639782510196	36.0	36.0	36.0	32.0	36.0
46	34.77797915722701	36.0	36.0	36.0	36.0	36.0
47	34.81286814680562	36.0	36.0	36.0	36.0	36.0
48	34.66379700951518	36.0	36.0	36.0	32.0	36.0
49	34.79497054825555	36.0	36.0	36.0	36.0	36.0
50	34.49592206615315	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	3.0
25	4.0
26	5.0
27	21.0
28	28.0
29	54.0
30	72.0
31	127.0
32	156.0
33	249.0
34	570.0
35	3123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52754477442757	18.77125368397189	11.856721831784176	25.84447970981637
2	18.3960126869053	19.93656547349343	35.04757589487993	26.619845944721344
3	18.849116447666518	23.878568192115996	29.497054825555054	27.775260534662436
4	13.525147258722248	17.172632532850024	35.52333484367921	33.778885364748525
5	15.15632079746262	34.798368826461264	34.798368826461264	15.246941549614862
6	32.736746714997736	35.59130040779338	18.146805618486635	13.525147258722248
7	29.225192569098326	29.74626189397372	22.541912097870412	18.486633439057545
8	28.18305391934753	33.14454009968283	20.25373810602628	18.418667874943363
9	24.05980969642048	16.198459447213413	20.57091073855913	39.170820117806976
10	15.90394200271862	25.73629361123697	32.94064340734028	25.419120978704125
11	34.59447213411872	22.42863615768011	23.402809243316717	19.57408246488446
12	23.674671499773446	25.283189850475758	28.568192115994563	22.47394653375623
13	29.1798821930222	20.299048482102403	26.189397371998187	24.33167195287721
14	23.674671499773446	20.27639329406434	24.309016764839146	31.739918441323063
15	25.056637970095153	26.95967376529225	23.312188491164477	24.67149977344812
16	24.44494789306751	27.25419120978704	23.923878568192116	24.376982328953332
17	23.221567739012233	26.84639782510195	26.5292251925691	23.402809243316717
18	22.38332578160399	26.0081558676937	26.5292251925691	25.079293158133215
19	24.309016764839146	24.512913457181696	27.661984594472134	23.516085183507023
20	26.280018124150427	25.487086542818304	25.441776166742187	22.791119166289082
21	25.373810602627998	25.690983235160854	24.127775260534662	24.807430901676483
22	23.833257816039875	26.347983688264616	25.64567285908473	24.173085636610782
23	22.76846397825102	25.577707294970548	26.50657000453104	25.147258722247397
24	24.60910944935418	25.424881033310676	27.804214819850444	22.161794697484705
25	23.53874037154508	24.603534209333937	27.072949705482557	24.784775713638425
26	24.241051200724968	26.37063887630267	25.781603987313094	23.606705935659267
27	25.30584503851382	25.758948799275032	26.053466243769822	22.881739918441323
28	23.991844132306298	25.237879474399637	26.21205256003625	24.558223833257816
29	24.467603081105572	24.422292705029452	26.393294064340733	24.71681014952424
30	24.05980969642048	26.393294064340733	25.66832804712279	23.878568192115996
31	23.10829179882193	26.86905301314001	24.830086089714545	25.192569098323514
32	24.512913457181696	25.8948799275034	25.849569551427276	23.74263706388763
33	24.762120525600363	25.826914363389214	26.347983688264616	23.06298142274581
34	23.833257816039875	24.96601721794291	26.23470774807431	24.96601721794291
35	24.467603081105572	25.66832804712279	25.192569098323514	24.67149977344812
36	24.762120525600363	26.80108744902583	25.962845491617582	22.47394653375623
37	23.991844132306298	27.412777526053468	24.422292705029452	24.173085636610782
38	23.425464431354783	26.93701857725419	26.121431807884004	23.516085183507023
39	25.215224286361575	25.192569098323514	26.438604440416857	23.15360217489805
40	24.422292705029452	24.35432714091527	26.076121431807884	25.147258722247397
41	24.762120525600363	25.62301767104667	26.166742183960128	23.44811961939284
42	24.01449932034436	25.62301767104667	25.804259175351156	24.558223833257816
43	24.422292705029452	25.64567285908473	26.50657000453104	23.425464431354783
44	24.790392023566735	25.900747790618627	25.53818264219352	23.77067754362112
45	23.44811961939284	25.16991391028546	26.574535568645217	24.807430901676483
46	25.64567285908473	25.60036248300861	25.283189850475758	23.470774807430903
47	24.218396012686906	26.23470774807431	25.781603987313094	23.76529225192569
48	22.995015858631625	27.299501585863162	25.94019030357952	23.76529225192569
49	23.584050747621205	25.94019030357952	26.144086995922066	24.33167195287721
50	23.017671046669687	26.755777072949705	25.826914363389214	24.39963751699139
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	2.0
15	3.0
16	4.0
17	3.5
18	3.0
19	8.0
20	13.0
21	15.5
22	18.0
23	26.5
24	35.0
25	45.0
26	55.0
27	60.5
28	66.0
29	93.0
30	120.0
31	140.5
32	161.0
33	161.5
34	162.0
35	188.5
36	215.0
37	219.5
38	224.0
39	257.5
40	291.0
41	268.5
42	246.0
43	251.0
44	256.0
45	242.0
46	228.0
47	213.5
48	199.0
49	203.5
50	208.0
51	221.5
52	235.0
53	227.5
54	220.0
55	216.0
56	212.0
57	223.5
58	235.0
59	219.5
60	204.0
61	193.0
62	182.0
63	173.5
64	165.0
65	151.5
66	138.0
67	124.5
68	111.0
69	97.0
70	83.0
71	60.5
72	38.0
73	37.5
74	37.0
75	28.0
76	19.0
77	15.5
78	12.0
79	8.5
80	5.0
81	4.5
82	4.0
83	3.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06796556411418216
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.022655188038060717
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.022655188038060717
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4414.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.52695967376529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.38613861386139	83.64295423652017
2	8.01980198019802	14.680561848663343
3	0.5445544554455445	1.4952424105120072
4	0.04950495049504951	0.18124150430448574
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.022655188038060717	0.0
34	0.0	0.0	0.0	0.022655188038060717	0.0
35	0.0	0.0	0.0	0.09062075215224287	0.0
36	0.0	0.0	0.0	0.11327594019030358	0.0
37	0.0	0.0	0.0	0.13593112822836428	0.0
38	0.0	0.0	0.0	0.13593112822836428	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE