FastQCFastQC Report
Thu 2 Feb 2017
SRR4061958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262789
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41920.12847904047733397No Hit
GCGCAAGACGGACCAGAGCGAAAGC37050.11355315958218566No Hit
GGGTAGGCACACGCTGAGCCAGTCA36110.11067218873178743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.014.4265431
TCGTATA500.001502227913.2975722
GTATCAA41150.012.8310631
CAAGACG7600.012.375214
GCGTTAT2100.012.209621
ACGGTAT4800.011.8762959
AAGACGG7850.011.73959355
TATACTG2700.011.6118425
CGGTCCA5000.011.59108710
CGTTATT2250.011.397922
CGTATAC855.3325275E-511.1752883
CGCAAGA8000.011.1604622
TATACCG600.00587524711.0840315
ACGGTGC1408.349161E-910.8583278
CGAATTT1053.4693494E-610.8571615
TAGGACG1158.823972E-710.7393134
GTCTTAG2850.010.6625931
CCGACCA6150.010.6596999
TCGGCGT2150.010.60499313
CGGACCA8500.010.5070289
Produced by FastQC (version 0.11.5)