##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061958_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3262789
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267051592977666	32.0	32.0	32.0	32.0	32.0
2	30.769065973925986	32.0	32.0	32.0	32.0	32.0
3	30.805873747888693	32.0	32.0	32.0	32.0	32.0
4	30.81614747383297	32.0	32.0	32.0	32.0	32.0
5	30.703449410918083	32.0	32.0	32.0	32.0	32.0
6	34.38336895214493	36.0	36.0	36.0	32.0	36.0
7	34.323632941020705	36.0	36.0	36.0	32.0	36.0
8	34.267714216273255	36.0	36.0	36.0	32.0	36.0
9	34.42969772179568	36.0	36.0	36.0	32.0	36.0
10	34.08895671770378	36.0	36.0	36.0	32.0	36.0
11	34.40768986287498	36.0	36.0	36.0	32.0	36.0
12	34.2174467916865	36.0	36.0	36.0	32.0	36.0
13	34.31253844487033	36.0	36.0	36.0	32.0	36.0
14	34.20519224503944	36.0	36.0	36.0	32.0	36.0
15	34.15329645894969	36.0	36.0	36.0	32.0	36.0
16	34.15546822059287	36.0	36.0	36.0	32.0	36.0
17	34.08879397349936	36.0	36.0	36.0	32.0	36.0
18	34.09498806082772	36.0	36.0	36.0	32.0	36.0
19	34.10020782833337	36.0	36.0	36.0	32.0	36.0
20	34.08930856393104	36.0	36.0	36.0	32.0	36.0
21	34.075224907280244	36.0	36.0	36.0	32.0	36.0
22	34.04050430475277	36.0	36.0	36.0	32.0	36.0
23	34.00309305934279	36.0	36.0	36.0	32.0	36.0
24	33.983543220232754	36.0	36.0	36.0	32.0	36.0
25	33.6260797127856	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	42.0
5	258.0
6	703.0
7	162.0
8	573.0
9	391.0
10	231.0
11	67.0
12	128.0
13	129.0
14	388.0
15	567.0
16	977.0
17	1296.0
18	1857.0
19	2628.0
20	4093.0
21	6128.0
22	9527.0
23	14024.0
24	20184.0
25	28310.0
26	39079.0
27	49735.0
28	65688.0
29	86391.0
30	114414.0
31	154541.0
32	219129.0
33	308660.0
34	673966.0
35	1458521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62290821267884	17.021693290343144	11.785880665448884	25.569517831529136
2	17.127540696815544	18.820449762871363	37.387741641652255	26.66426789866084
3	20.240452647035656	22.075584404996622	27.117936546417866	30.566026401549852
4	13.3117892236948	15.024119880310957	34.31588613969729	37.34820475629695
5	15.532196696784162	35.80003495142059	32.69702330401297	15.97074504778227
6	35.84107367335729	33.85115178160971	16.13947915360083	14.168295391432167
7	31.890102115358477	29.312566021538633	19.17015432203876	19.627177541064132
8	28.547106624059793	32.90727482200687	18.39871381650101	20.14690473743233
9	26.72652972052112	15.14728164580849	18.44882193707629	39.6773666965941
10	16.959665366473427	26.406525722036893	29.940248397263762	26.69356051422592
11	37.43125516957749	21.547489522733866	21.225813282917645	19.795442024771003
12	25.015482346442436	23.309846404075433	27.80841171686793	23.8662595326142
13	29.302756060519116	19.55022796089083	24.814732868256495	26.33228311033356
14	23.762996601744387	19.3040863448424	24.51101172896802	32.42190532444519
15	25.377976357622945	27.06241669789297	21.63797069238916	25.921636252094927
16	26.31433274980937	25.97603204727925	22.83334893147608	24.876286271435298
17	24.37890562188609	26.15958074902018	24.45482397620693	25.0066896528868
18	24.96147787501465	25.24044494818613	25.31380727462179	24.484269902177427
19	25.749488744400423	25.01293094720799	24.81511659940938	24.422463708982207
20	25.98869865850254	24.417830596449463	24.528942048414162	25.064528696633833
21	26.735212436771754	24.175651577987352	24.300842654664358	24.788293330576533
22	26.21115660934748	24.176284036623166	24.73714260644455	24.875416747584804
23	24.78518488072098	24.43636432791463	25.108362276462064	25.67008851490232
24	24.72864683257183	24.90396573504789	25.071707425115974	25.29568000726431
25	25.036121027990653	24.522849656152353	24.94489050803804	25.49613880781895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	190.0
2	466.0
3	742.0
4	742.0
5	742.0
6	1550.0
7	2358.0
8	2358.0
9	2358.0
10	2902.0
11	3446.0
12	3446.0
13	3446.0
14	5010.0
15	6574.0
16	6574.0
17	6574.0
18	11407.0
19	16240.0
20	16240.0
21	16240.0
22	26307.5
23	36375.0
24	36375.0
25	36375.0
26	54891.0
27	73407.0
28	73407.0
29	73407.0
30	96455.0
31	119503.0
32	119503.0
33	119503.0
34	152297.5
35	185092.0
36	185092.0
37	185092.0
38	222633.5
39	260175.0
40	260175.0
41	260175.0
42	298747.5
43	337320.0
44	337320.0
45	337320.0
46	376011.5
47	414703.0
48	414703.0
49	414703.0
50	435785.5
51	456868.0
52	456868.0
53	456868.0
54	431144.5
55	405421.0
56	405421.0
57	405421.0
58	376122.0
59	346823.0
60	346823.0
61	346823.0
62	304060.5
63	261298.0
64	261298.0
65	261298.0
66	213955.0
67	166612.0
68	166612.0
69	166612.0
70	126237.0
71	85862.0
72	85862.0
73	85862.0
74	64845.0
75	43828.0
76	43828.0
77	43828.0
78	33852.5
79	23877.0
80	23877.0
81	23877.0
82	16522.5
83	9168.0
84	9168.0
85	9168.0
86	6620.5
87	4073.0
88	4073.0
89	4073.0
90	2684.5
91	1296.0
92	1296.0
93	1296.0
94	818.5
95	341.0
96	341.0
97	341.0
98	769.0
99	1197.0
100	1197.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.888101559739229E-4
2	9.194587820419893E-5
3	7.968642777697239E-4
4	0.010267289732802214
5	0.0342958125701662
6	0.06307487244808047
7	0.10530867916987584
8	0.10831224452454633
9	0.11631153592831164
10	0.13175844346661705
11	0.1392673568532933
12	0.12991952590253308
13	0.12614974489616093
14	0.11536142852020158
15	0.1323407673619103
16	0.11811980486632755
17	0.12336071992396688
18	0.11103997224460424
19	0.097064198757566
20	0.09191522957813085
21	0.09108771667429306
22	0.10181473579811628
23	0.08152534534105638
24	0.09240560759521992
25	0.0893407449884133
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3262789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.83086790465423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.47529619623475	23.010193815076686
2	16.18627726136109	11.923092793625973
3	7.511325776528807	8.299459423924704
4	4.120955430265373	6.071134603722859
5	2.4414049594836262	4.495953178225457
6	1.648906554617833	3.6438395700148756
7	1.124806946261772	2.899933123920338
8	0.7744030035953514	2.281754778431029
9	0.6017633103419913	1.9947118493765994
>10	2.8416301075206336	18.194659440625745
>50	0.16396391530382426	4.135503730334235
>100	0.09384101728677863	6.84621988053023
>500	0.009557116394980851	2.4715122089465376
>1k	0.005868404803386618	3.7320316032446623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4192	0.12847904047733397	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3705	0.11355315958218566	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3611	0.11067218873178743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.129725213613262E-5
2	0.0	0.0	0.0	0.0	6.129725213613262E-5
3	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
4	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
5	0.0	3.064862606806631E-5	0.0	0.0	9.194587820419893E-5
6	0.0	3.064862606806631E-5	0.0	0.0	9.194587820419893E-5
7	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	9.194587820419893E-5
8	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	9.194587820419893E-5
9	0.0	3.064862606806631E-5	0.0	6.129725213613262E-5	9.194587820419893E-5
10	3.064862606806631E-5	3.064862606806631E-5	0.0	6.129725213613262E-5	9.194587820419893E-5
11	3.064862606806631E-5	3.064862606806631E-5	0.0	6.129725213613262E-5	9.194587820419893E-5
12	3.064862606806631E-5	3.064862606806631E-5	0.0	6.129725213613262E-5	2.4518900854453047E-4
13	3.064862606806631E-5	6.129725213613262E-5	0.0	6.129725213613262E-5	3.677835128167957E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1435	0.0	14.426543	1
TCGTATA	50	0.0015022279	13.297572	2
GTATCAA	4115	0.0	12.831063	1
CAAGACG	760	0.0	12.37521	4
GCGTTAT	210	0.0	12.20962	1
ACGGTAT	480	0.0	11.876295	9
AAGACGG	785	0.0	11.7395935	5
TATACTG	270	0.0	11.611842	5
CGGTCCA	500	0.0	11.591087	10
CGTTATT	225	0.0	11.39792	2
CGTATAC	85	5.3325275E-5	11.175288	3
CGCAAGA	800	0.0	11.160462	2
TATACCG	60	0.005875247	11.084031	5
ACGGTGC	140	8.349161E-9	10.858327	8
CGAATTT	105	3.4693494E-6	10.85716	15
TAGGACG	115	8.823972E-7	10.739313	4
GTCTTAG	285	0.0	10.662593	1
CCGACCA	615	0.0	10.659699	9
TCGGCGT	215	0.0	10.604993	13
CGGACCA	850	0.0	10.507028	9
>>END_MODULE