FastQCFastQC Report
Thu 2 Feb 2017
SRR4061958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262789
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT38070.11667931944112843No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC37230.11410483485141086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36800.029.0064471
GGTATCA14200.028.5187241
CGCAATA5650.019.0801536
TCAACGC57100.018.377084
ATCAACG57550.018.3098353
CTAGCGG5900.018.2708329
ATACGAA6200.018.09891940
TATCAAC58950.018.0624122
CAACGCA58700.017.9136495
CGAATGC6200.017.74376543
TACGAAT6350.017.32515141
AACGCAG61100.017.1739986
GTATAGG4800.016.9652821
TATAGGG3300.016.6663422
TCTAGCG6500.016.584828
TAGCGGC6650.016.5410330
GCGCAAT6600.016.33251435
CAATACG6750.015.97226238
CGTCGTA9000.015.88784910
ACCGTCG9500.015.7463398
Produced by FastQC (version 0.11.5)