##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3262789
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27815405777082	32.0	32.0	32.0	32.0	32.0
2	31.451854226552804	32.0	32.0	32.0	32.0	32.0
3	31.575796044427022	32.0	32.0	32.0	32.0	32.0
4	31.654155693181508	32.0	32.0	32.0	32.0	32.0
5	31.627399136137825	32.0	32.0	32.0	32.0	32.0
6	35.27927487802613	36.0	36.0	36.0	36.0	36.0
7	35.28428194406687	36.0	36.0	36.0	36.0	36.0
8	35.23253419084102	36.0	36.0	36.0	36.0	36.0
9	35.31862495552118	36.0	36.0	36.0	36.0	36.0
10	35.20519163206692	36.0	36.0	36.0	36.0	36.0
11	35.32095149272601	36.0	36.0	36.0	36.0	36.0
12	35.24689307215392	36.0	36.0	36.0	36.0	36.0
13	35.2827718862605	36.0	36.0	36.0	36.0	36.0
14	35.24095306193566	36.0	36.0	36.0	36.0	36.0
15	35.2186105200183	36.0	36.0	36.0	36.0	36.0
16	35.23305552397044	36.0	36.0	36.0	36.0	36.0
17	35.21060356645802	36.0	36.0	36.0	36.0	36.0
18	35.206872096234235	36.0	36.0	36.0	36.0	36.0
19	35.206502780290116	36.0	36.0	36.0	36.0	36.0
20	35.20595018556211	36.0	36.0	36.0	36.0	36.0
21	35.194669345765234	36.0	36.0	36.0	36.0	36.0
22	35.18349669561838	36.0	36.0	36.0	36.0	36.0
23	35.15310429206424	36.0	36.0	36.0	36.0	36.0
24	35.13941293782712	36.0	36.0	36.0	36.0	36.0
25	35.1219870485036	36.0	36.0	36.0	36.0	36.0
26	35.07836087469953	36.0	36.0	36.0	36.0	36.0
27	35.0624456561549	36.0	36.0	36.0	36.0	36.0
28	35.04722187061437	36.0	36.0	36.0	36.0	36.0
29	35.02813268035414	36.0	36.0	36.0	36.0	36.0
30	35.013798317942104	36.0	36.0	36.0	36.0	36.0
31	35.017102546318505	36.0	36.0	36.0	36.0	36.0
32	34.99276232695403	36.0	36.0	36.0	36.0	36.0
33	34.971285608723086	36.0	36.0	36.0	36.0	36.0
34	34.96352752200648	36.0	36.0	36.0	36.0	36.0
35	34.95096127883231	36.0	36.0	36.0	36.0	36.0
36	34.935182446673686	36.0	36.0	36.0	36.0	36.0
37	34.919640528394574	36.0	36.0	36.0	36.0	36.0
38	34.903449472215335	36.0	36.0	36.0	36.0	36.0
39	34.888195038048735	36.0	36.0	36.0	32.0	36.0
40	34.8760931215595	36.0	36.0	36.0	32.0	36.0
41	34.85411560477861	36.0	36.0	36.0	32.0	36.0
42	34.830428507635645	36.0	36.0	36.0	32.0	36.0
43	34.82155235904007	36.0	36.0	36.0	32.0	36.0
44	34.78645631084327	36.0	36.0	36.0	32.0	36.0
45	34.76873006498428	36.0	36.0	36.0	32.0	36.0
46	34.75247863101169	36.0	36.0	36.0	32.0	36.0
47	34.73168231227947	36.0	36.0	36.0	32.0	36.0
48	34.702634463950936	36.0	36.0	36.0	32.0	36.0
49	34.700431747195424	36.0	36.0	36.0	32.0	36.0
50	34.28918909558663	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	26.0
21	74.0
22	229.0
23	606.0
24	1483.0
25	3541.0
26	7417.0
27	14097.0
28	24435.0
29	38675.0
30	57897.0
31	85229.0
32	130317.0
33	223458.0
34	517763.0
35	2157538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21931052793425	17.552709266572503	12.193562094519047	26.0344181109742
2	16.541578074805503	19.2587799808804	37.111604443176205	27.08803750113789
3	19.656127491319516	22.453436165918685	27.43553884587341	30.45489749688839
4	13.056686490204083	15.360758958289658	34.4192757850035	37.163278766502756
5	15.226298727867476	36.142637479775736	32.560977740209374	16.07008605214741
6	36.082406926109726	33.88581652968753	15.979666444361627	14.052110099841117
7	31.903687305553625	29.27716134877248	19.190637212519718	19.62851413315418
8	28.60794859857625	32.91745191000705	18.311971751774326	20.162627739642375
9	26.781688917058382	14.959257248936417	18.338789299583883	39.92026453442132
10	16.87327946117214	26.411232609187362	30.040431704684277	26.67505622495622
11	37.656403769903605	21.355288374455107	21.22257982358038	19.765728032060913
12	25.037421983898433	23.16678251850871	27.863471362527996	23.93232413506486
13	29.49029189444981	19.30667291081342	24.89180268782321	26.311232506913562
14	23.865778632942554	19.125846017011828	24.490612172592222	32.5177631774534
15	25.41779440840336	27.01087934279538	21.589259985858725	25.98206626294253
16	26.40324656358257	25.865084699425918	22.847038393415662	24.884630343575846
17	24.52549030905768	26.030797578390757	24.43213459405435	25.011577518497212
18	25.128149803404025	25.08993089641573	25.271616285851678	24.510303014328567
19	25.858061921871133	24.83298797439859	24.810767720499243	24.498182383231036
20	26.189958811210435	24.18951808288696	24.55092756761793	25.069595538284673
21	26.787436630095684	24.049685131147083	24.357274930910293	24.80560330784694
22	26.253950224792348	24.059355355188462	24.79783400029852	24.888860419720675
23	24.84026692517777	24.317983368206384	25.13556661835419	25.706183088261657
24	24.85534463972931	24.716811406311535	25.101271777991297	25.326572175967854
25	25.181823364233345	24.28773972640526	25.032441640293467	25.49799526906793
26	24.923508269504662	25.130019851224617	25.095693200656743	24.850778678613974
27	25.263985734824786	24.6499677727919	24.976745226680023	25.109301265703294
28	25.154341278503296	24.46908837580228	25.114252236695755	25.26231810899867
29	24.799150660068182	24.721118779784902	25.269609953999193	25.210120606147722
30	24.926166350981077	24.86134353334927	25.291718003886306	24.92077211178335
31	25.458237236708015	24.590754802277203	24.627441365095663	25.323566595919118
32	25.422364160458997	24.58776528496536	24.475283999920315	25.514586554655335
33	24.851982142846197	24.538507322891938	24.933415717016953	25.676094817244916
34	25.460163125897246	24.70377803304936	24.932480393875604	24.903578447177786
35	25.67482248288578	24.386505511857358	25.1610587767992	24.77761322845766
36	24.776731463248307	24.89944908858257	24.899816873673483	25.424002574495635
37	25.665638489462207	24.532814831251848	24.89232949564966	24.90921718363629
38	25.055198192466076	24.289595278639002	25.059918082327137	25.595288446567782
39	25.688999605856853	24.46620575007647	24.53957889820135	25.30521574586533
40	25.65903055212035	24.594510517855145	24.76277168124777	24.983687248776736
41	24.761597397048753	25.19647488413177	25.257987261084025	24.783940457735447
42	25.98555086300353	24.798804150165342	24.73036216019226	24.48528282663886
43	25.477356777641763	24.138600047691234	24.911406480736233	25.472636693930767
44	24.90141449435275	24.802997563330422	24.708442523715384	25.58714541860144
45	25.211004799060415	24.880728422464433	24.712647905644427	25.19561887283072
46	25.116252697136133	24.703621518242446	24.732431836014122	25.4476939486073
47	25.09970868177353	24.68962579170057	25.075158876470958	25.13550665005494
48	25.596199184225917	25.02927052338761	24.22532724751645	25.149203044870028
49	25.085849669596772	25.020473966187307	24.481224024421643	25.412452339794278
50	25.229930009574797	25.084040000833657	24.468635487070344	25.217394502521202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	43.5
2	47.0
3	155.5
4	264.0
5	295.5
6	327.0
7	286.0
8	245.0
9	341.5
10	438.0
11	709.0
12	980.0
13	1750.0
14	2520.0
15	3331.5
16	4143.0
17	4057.5
18	3972.0
19	4002.5
20	4033.0
21	4750.5
22	5468.0
23	7048.5
24	8629.0
25	11398.5
26	14168.0
27	18321.5
28	22475.0
29	27139.0
30	31803.0
31	38318.0
32	44833.0
33	53202.5
34	61572.0
35	73148.0
36	84724.0
37	98078.0
38	111432.0
39	124357.5
40	137283.0
41	149768.0
42	162253.0
43	170841.0
44	179429.0
45	195755.5
46	212082.0
47	225542.5
48	239003.0
49	248094.0
50	257185.0
51	253909.0
52	250633.0
53	252432.5
54	254232.0
55	253153.5
56	252075.0
57	240588.5
58	229102.0
59	213607.0
60	198112.0
61	175801.5
62	153491.0
63	130948.5
64	108406.0
65	91478.0
66	74550.0
67	64864.5
68	55179.0
69	47709.0
70	40239.0
71	30662.0
72	21085.0
73	18325.0
74	15565.0
75	11482.0
76	7399.0
77	6244.0
78	5089.0
79	4365.5
80	3642.0
81	2849.0
82	2056.0
83	1676.0
84	1296.0
85	1042.5
86	789.0
87	529.0
88	269.0
89	173.5
90	78.0
91	62.5
92	47.0
93	39.0
94	31.0
95	32.0
96	33.0
97	32.0
98	31.0
99	21.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03297792164923934
2	0.00514896917943514
3	3.064862606806631E-4
4	3.064862606806631E-5
5	0.0
6	1.5324313034033156E-4
7	0.0
8	0.0
9	0.0
10	9.194587820419893E-5
11	0.0
12	3.064862606806631E-5
13	0.0
14	0.0
15	0.0
16	4.903780170890609E-4
17	0.0
18	1.8389175640839785E-4
19	0.0
20	2.4518900854453047E-4
21	6.129725213613262E-5
22	0.0
23	6.129725213613262E-5
24	0.0010420532863142545
25	2.1454038247646414E-4
26	5.516752692251935E-4
27	5.516752692251935E-4
28	0.0015937285555394478
29	6.129725213613262E-4
30	0.001501782677335249
31	4.290807649529283E-4
32	7.355670256335914E-4
33	2.1454038247646414E-4
34	0.001011404660246188
35	1.5324313034033156E-4
36	4.290807649529283E-4
37	0.0017469716858797796
38	3.064862606806631E-5
39	4.597293910209946E-4
40	1.2259450427226523E-4
41	9.501074081100556E-4
42	0.005271563683707405
43	0.004137564519188951
44	0.004260159023461217
45	0.0020534579465604427
46	0.002114755198696575
47	0.0010420532863142545
48	0.0037084837542360233
49	0.0033407002414192273
50	0.0017469716858797796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3262789.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.16523149769515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.29408168701321	22.780087353364834
2	16.73047683503884	12.435840892820892
3	7.743668235257717	8.633856678141047
4	4.204666247886541	6.250695778929943
5	2.6320024307393433	4.8909489820462015
6	1.6766576534468742	3.738802189964125
7	1.1275311616863561	2.9333469651456996
8	0.8283388651195902	2.4628324544566125
9	0.6102238179727205	2.041119851432719
>10	2.9038289582595374	18.60101190314564
>50	0.15003848285364668	3.813961471449047
>100	0.08465113994741787	6.045304853331595
>500	0.008946852432197535	2.2922381153450266
>1k	0.004887632346135631	3.0799525104266596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3807	0.11667931944112843	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3723	0.11410483485141086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.129725213613262E-5
2	0.0	0.0	0.0	0.0	6.129725213613262E-5
3	0.0	0.0	0.0	0.0	6.129725213613262E-5
4	0.0	0.0	0.0	0.0	6.129725213613262E-5
5	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
6	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
7	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
8	0.0	3.064862606806631E-5	0.0	0.0	6.129725213613262E-5
9	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	6.129725213613262E-5
10	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	6.129725213613262E-5
11	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	9.194587820419893E-5
12	0.0	3.064862606806631E-5	0.0	3.064862606806631E-5	2.4518900854453047E-4
13	0.0	3.064862606806631E-5	0.0	6.129725213613262E-5	3.0648626068066306E-4
14	0.0	3.064862606806631E-5	0.0	9.194587820419893E-5	3.0648626068066306E-4
15	0.0	3.064862606806631E-5	0.0	3.0648626068066306E-4	3.98432138884862E-4
16	0.0	3.064862606806631E-5	0.0	4.5972939102099464E-4	4.290807649529283E-4
17	0.0	3.064862606806631E-5	0.0	6.129725213613261E-4	4.290807649529283E-4
18	0.0	3.064862606806631E-5	0.0	7.96864277769724E-4	4.903780170890609E-4
19	0.0	3.064862606806631E-5	0.0	9.194587820419893E-4	4.903780170890609E-4
20	0.0	3.064862606806631E-5	0.0	0.0014098367991310502	5.210266431571272E-4
21	0.0	3.064862606806631E-5	0.0	0.0018695661901520447	5.516752692251935E-4
22	0.0	3.064862606806631E-5	0.0	0.002390592833309172	5.823238952932598E-4
23	0.0	3.064862606806631E-5	0.0	0.0030648626068066308	5.823238952932598E-4
24	0.0	9.194587820419893E-5	0.0	0.004045618640984752	6.129725213613261E-4
25	0.0	9.194587820419893E-5	0.0	0.0050263746751628745	6.129725213613261E-4
26	0.0	9.194587820419893E-5	0.0	0.0057312930747284	6.742697734974588E-4
27	0.0	9.194587820419893E-5	0.0	0.007110481247791383	6.742697734974588E-4
28	0.0	9.194587820419893E-5	0.0	0.008029940029833373	6.742697734974588E-4
29	0.0	9.194587820419893E-5	0.0	0.010359235611006412	6.742697734974588E-4
30	0.0	9.194587820419893E-5	0.0	0.012412693557566854	7.049183995655251E-4
31	0.0	9.194587820419893E-5	0.0	0.015446907538305419	7.049183995655251E-4
32	0.0	9.194587820419893E-5	0.0	0.020105498700651498	7.662156517016577E-4
33	0.0	9.194587820419893E-5	0.0	0.02439630635018078	7.662156517016577E-4
34	0.0	1.5324313034033153E-4	0.0	0.02930008652107139	7.96864277769724E-4
35	0.0	1.5324313034033153E-4	0.0	0.03484748783939139	7.96864277769724E-4
36	0.0	1.5324313034033153E-4	0.0	0.042478995730339905	7.96864277769724E-4
37	0.0	1.5324313034033153E-4	0.0	0.05473844615756643	7.96864277769724E-4
38	0.0	1.5324313034033153E-4	0.0	0.06880616552280885	7.96864277769724E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3680	0.0	29.006447	1
GGTATCA	1420	0.0	28.518724	1
CGCAATA	565	0.0	19.08015	36
TCAACGC	5710	0.0	18.37708	4
ATCAACG	5755	0.0	18.309835	3
CTAGCGG	590	0.0	18.27083	29
ATACGAA	620	0.0	18.098919	40
TATCAAC	5895	0.0	18.062412	2
CAACGCA	5870	0.0	17.913649	5
CGAATGC	620	0.0	17.743765	43
TACGAAT	635	0.0	17.325151	41
AACGCAG	6110	0.0	17.173998	6
GTATAGG	480	0.0	16.965282	1
TATAGGG	330	0.0	16.666342	2
TCTAGCG	650	0.0	16.5848	28
TAGCGGC	665	0.0	16.54103	30
GCGCAAT	660	0.0	16.332514	35
CAATACG	675	0.0	15.972262	38
CGTCGTA	900	0.0	15.887849	10
ACCGTCG	950	0.0	15.746339	8
>>END_MODULE