##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061957_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2973
Sequences flagged as poor quality	0
Sequence length	25
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394214598049107	32.0	32.0	32.0	32.0	32.0
2	31.197780020181636	32.0	32.0	32.0	32.0	32.0
3	31.15472586612849	32.0	32.0	32.0	32.0	32.0
4	31.14026236125126	32.0	32.0	32.0	32.0	32.0
5	31.124789774638412	32.0	32.0	32.0	32.0	32.0
6	34.56575849310461	36.0	36.0	36.0	32.0	36.0
7	34.60511268079381	36.0	36.0	36.0	32.0	36.0
8	34.504204507231755	36.0	36.0	36.0	32.0	36.0
9	34.64480322906155	36.0	36.0	36.0	32.0	36.0
10	34.39354187689203	36.0	36.0	36.0	32.0	36.0
11	34.52875882946519	36.0	36.0	36.0	32.0	36.0
12	34.595358224016145	36.0	36.0	36.0	32.0	36.0
13	34.39017827110663	36.0	36.0	36.0	32.0	36.0
14	34.56979482004709	36.0	36.0	36.0	32.0	36.0
15	34.52169525731584	36.0	36.0	36.0	32.0	36.0
16	34.3790783720148	36.0	36.0	36.0	32.0	36.0
17	34.37941473259334	36.0	36.0	36.0	32.0	36.0
18	34.418768920282545	36.0	36.0	36.0	32.0	36.0
19	34.18230743356879	36.0	36.0	36.0	32.0	36.0
20	34.307097208207196	36.0	36.0	36.0	32.0	36.0
21	34.36495122771611	36.0	36.0	36.0	32.0	36.0
22	34.22468886646485	36.0	36.0	36.0	32.0	36.0
23	34.08207198116381	36.0	36.0	36.0	32.0	36.0
24	34.25193407332661	36.0	36.0	36.0	32.0	36.0
25	33.78506559031282	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.0
19	1.0
20	6.0
21	5.0
22	9.0
23	12.0
24	11.0
25	17.0
26	21.0
27	36.0
28	39.0
29	52.0
30	83.0
31	118.0
32	188.0
33	286.0
34	758.0
35	1323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74503868146653	18.197107299024555	10.023545240497814	26.034308779011102
2	20.181634712411707	17.79347460477632	34.10696266397578	27.917928018836193
3	21.358896737302388	20.820719811638075	26.807938109653552	31.012445341405986
4	15.539858728557013	16.279852001345443	33.13151698620921	35.04877228388833
5	17.306397306397304	31.64983164983165	33.6026936026936	17.44107744107744
6	35.319865319865315	32.55892255892256	16.296296296296298	15.824915824915825
7	31.42472212866285	27.382957224654763	19.198383294038397	21.99393735264399
8	29.235432805658473	29.639609296059277	18.322667564836646	22.802290333445605
9	24.02291105121294	13.780323450134771	18.564690026954178	43.63207547169811
10	18.025606469002696	20.99056603773585	29.582210242587603	31.40161725067385
11	40.03372681281619	19.190556492411467	21.45025295109612	19.32546374367622
12	23.896191439164138	21.166161105493764	27.165487023929895	27.772160431412203
13	32.09710047201618	17.194875252865813	23.162508428860416	27.545515846257583
14	23.98921832884097	18.53099730458221	21.69811320754717	35.78167115902965
15	28.263069139966273	23.204047217537944	19.595278246205734	28.93760539629005
16	27.131782945736433	24.098415908324906	20.626895854398384	28.142905291540277
17	26.617250673854446	23.045822102425877	22.708894878706197	27.628032345013477
18	27.046143482654095	23.341192320646684	21.657123610643314	27.95554058605591
19	27.367711493090663	22.716548702392988	22.24469160768453	27.671048196831816
20	26.473560121252948	23.84641293364769	22.26338834624453	27.41663859885483
21	28.258672953856518	22.90333445604581	21.72448635904345	27.11350623105423
22	26.776692489053556	21.791849107443582	23.576961940047152	27.854496463455707
23	26.86868686868687	23.03030303030303	22.087542087542086	28.013468013468014
24	26.540922869653084	23.913775682047827	22.499157965644997	27.046143482654095
25	27.315594476254628	23.071741327046144	22.6002020882452	27.012462108454027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	4.0
9	4.0
10	5.5
11	7.0
12	7.0
13	7.0
14	8.0
15	9.0
16	9.0
17	9.0
18	18.0
19	27.0
20	27.0
21	27.0
22	33.5
23	40.0
24	40.0
25	40.0
26	46.0
27	52.0
28	52.0
29	52.0
30	68.5
31	85.0
32	85.0
33	85.0
34	119.0
35	153.0
36	153.0
37	153.0
38	165.5
39	178.0
40	178.0
41	178.0
42	211.5
43	245.0
44	245.0
45	245.0
46	257.0
47	269.0
48	269.0
49	269.0
50	285.5
51	302.0
52	302.0
53	302.0
54	311.0
55	320.0
56	320.0
57	320.0
58	319.5
59	319.0
60	319.0
61	319.0
62	315.0
63	311.0
64	311.0
65	311.0
66	292.5
67	274.0
68	274.0
69	274.0
70	229.0
71	184.0
72	184.0
73	184.0
74	140.5
75	97.0
76	97.0
77	97.0
78	81.5
79	66.0
80	66.0
81	66.0
82	42.0
83	18.0
84	18.0
85	18.0
86	14.5
87	11.0
88	11.0
89	11.0
90	6.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.10090817356205853
6	0.10090817356205853
7	0.13454423141607805
8	0.13454423141607805
9	0.16818028927009754
10	0.16818028927009754
11	0.2690884628321561
12	0.20181634712411706
13	0.23545240497813655
14	0.16818028927009754
15	0.2690884628321561
16	0.20181634712411706
17	0.16818028927009754
18	0.13454423141607805
19	0.20181634712411706
20	0.13454423141607805
21	0.13454423141607805
22	0.13454423141607805
23	0.10090817356205853
24	0.13454423141607805
25	0.13454423141607805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.79549276824756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.64606741573034	91.62462159434914
2	4.318820224719102	8.274470232088799
3	0.0351123595505618	0.10090817356205853
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTCATTGACCCACAGCTTGTT	3	0.10090817356205853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE