##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2973
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353178607467203	32.0	32.0	32.0	32.0	32.0
2	31.38109653548604	32.0	32.0	32.0	32.0	32.0
3	31.461823074335687	32.0	32.0	32.0	32.0	32.0
4	31.59031281533804	32.0	32.0	32.0	32.0	32.0
5	31.564076690211905	32.0	32.0	32.0	32.0	32.0
6	35.18802556340397	36.0	36.0	36.0	36.0	36.0
7	35.15405314497141	36.0	36.0	36.0	36.0	36.0
8	35.264379414732595	36.0	36.0	36.0	36.0	36.0
9	35.29330642448705	36.0	36.0	36.0	36.0	36.0
10	35.18668012108981	36.0	36.0	36.0	36.0	36.0
11	35.294315506222674	36.0	36.0	36.0	36.0	36.0
12	35.20652539522368	36.0	36.0	36.0	36.0	36.0
13	35.29397914564413	36.0	36.0	36.0	36.0	36.0
14	35.196770938446015	36.0	36.0	36.0	36.0	36.0
15	35.171880255634036	36.0	36.0	36.0	36.0	36.0
16	35.07702657248571	36.0	36.0	36.0	36.0	36.0
17	35.076353851328626	36.0	36.0	36.0	36.0	36.0
18	35.135553313151696	36.0	36.0	36.0	36.0	36.0
19	35.1765893037336	36.0	36.0	36.0	36.0	36.0
20	35.122771611167174	36.0	36.0	36.0	36.0	36.0
21	35.07601749075008	36.0	36.0	36.0	36.0	36.0
22	35.14362596703666	36.0	36.0	36.0	36.0	36.0
23	35.13017154389505	36.0	36.0	36.0	36.0	36.0
24	35.09014463504877	36.0	36.0	36.0	36.0	36.0
25	35.15674402959973	36.0	36.0	36.0	36.0	36.0
26	34.88193743693239	36.0	36.0	36.0	32.0	36.0
27	34.91153716784393	36.0	36.0	36.0	32.0	36.0
28	34.894382778338375	36.0	36.0	36.0	32.0	36.0
29	34.84392869155735	36.0	36.0	36.0	32.0	36.0
30	34.97712748065927	36.0	36.0	36.0	32.0	36.0
31	35.03834510595358	36.0	36.0	36.0	36.0	36.0
32	34.856710393541874	36.0	36.0	36.0	32.0	36.0
33	34.85502859064918	36.0	36.0	36.0	32.0	36.0
34	34.817356205852676	36.0	36.0	36.0	32.0	36.0
35	34.87991927346115	36.0	36.0	36.0	32.0	36.0
36	34.823074335687856	36.0	36.0	36.0	32.0	36.0
37	34.840901446350486	36.0	36.0	36.0	32.0	36.0
38	34.839556004036325	36.0	36.0	36.0	32.0	36.0
39	34.72822065253952	36.0	36.0	36.0	32.0	36.0
40	34.90514631685166	36.0	36.0	36.0	32.0	36.0
41	34.84493777329297	36.0	36.0	36.0	32.0	36.0
42	34.815338042381434	36.0	36.0	36.0	32.0	36.0
43	34.61150353178608	36.0	36.0	36.0	32.0	36.0
44	34.67070299360915	36.0	36.0	36.0	32.0	36.0
45	34.70669357551295	36.0	36.0	36.0	32.0	36.0
46	34.57214934409687	36.0	36.0	36.0	32.0	36.0
47	34.72586612848974	36.0	36.0	36.0	32.0	36.0
48	34.66834846955937	36.0	36.0	36.0	32.0	36.0
49	34.66633030608813	36.0	36.0	36.0	32.0	36.0
50	34.06256306760848	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	1.0
25	2.0
26	7.0
27	11.0
28	26.0
29	31.0
30	51.0
31	84.0
32	139.0
33	210.0
34	583.0
35	1826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.530282637954244	19.818304172274562	11.709286675639301	25.942126514131896
2	20.887991927346114	18.096199125462494	31.146989572822065	29.868819374369327
3	21.05617221661621	21.493440968718467	26.2024890682812	31.247897746384123
4	15.338042381432896	16.313488059199464	32.694248234106965	35.65422132526068
5	17.12075344769593	33.40060544904137	31.247897746384123	18.230743356878573
6	35.21695257315843	33.871510258997645	16.851664984863774	14.059872182980154
7	31.348805919946184	26.303397241843257	20.080726538849646	22.267070299360913
8	29.70063908509923	28.59064917591658	17.89438277833838	23.81432896064581
9	25.36158762193071	14.160780356542213	17.961654894046415	42.51597712748066
10	18.836192398250926	24.217961654894047	26.505213588967376	30.440632357887655
11	39.95963673057518	20.047090480995628	21.12344433232425	18.869828456104944
12	24.453414059872184	21.728893373696604	26.26976118398924	27.547931382441977
13	33.501513622603426	18.230743356878573	22.334342415068953	25.93340060544904
14	25.15977127480659	18.46619576185671	21.25798856374033	35.11604439959637
15	29.297006390850992	24.386141944164144	18.46619576185671	27.850655903128153
16	28.523377060208542	23.84796501849983	21.527077026572485	26.101580894719138
17	25.22704339051463	24.587958291288263	23.37706020854356	26.807938109653552
18	26.303397241843257	24.04978136562395	23.81432896064581	25.832492431886983
19	27.413387151025898	24.520686175580224	21.05617221661621	27.009754456777667
20	26.437941473259336	23.747056844937774	23.040699630003363	26.77430205179953
21	26.2024890682812	23.007063572149345	21.560713084426506	29.229734275142956
22	28.187016481668348	23.242515977127482	21.19071644803229	27.379751093171876
23	26.437941473259336	24.251597712748065	23.175243861419442	26.13521695257316
24	26.97611839892365	22.53615876219307	21.863437605112683	28.624285233770603
25	27.514295324587955	23.578876555667673	22.737975109317187	26.168853010427178
26	28.893373696602758	23.646148671375713	22.53615876219307	24.924318869828458
27	28.59064917591658	21.82980154725866	21.82980154725866	27.749747729566092
28	28.388832828792467	23.20887991927346	20.955264043054154	27.447023208879916
29	29.263370332996974	22.906155398587284	21.96434577867474	25.866128489741
30	27.077026572485707	23.040699630003363	22.805247225025226	27.077026572485707
31	27.88429196098217	22.334342415068953	22.233434241506895	27.547931382441977
32	26.505213588967376	23.14160780356542	21.8970736629667	28.456104944500503
33	28.05247225025227	22.267070299360913	22.267070299360913	27.413387151025898
34	27.27884291960982	22.56979482004709	21.863437605112683	28.287924655230405
35	26.437941473259336	23.242515977127482	23.040699630003363	27.27884291960982
36	28.388832828792467	23.444332324251597	22.36797847292297	25.798856374032965
37	27.682475613858053	23.007063572149345	21.426168853010427	27.88429196098217
38	27.581567440295995	21.863437605112683	23.578876555667673	26.97611839892365
39	27.27884291960982	22.166162125798856	23.30978809283552	27.2452068617558
40	27.447023208879916	22.63706693575513	23.2761520349815	26.63975782038345
41	28.489741002354524	22.906155398587284	22.199798183652874	26.404305415405315
42	26.303397241843257	23.242515977127482	21.82980154725866	28.624285233770603
43	26.673393878237473	23.040699630003363	22.468886646485032	27.81701984527413
44	27.88429196098217	22.70433905146317	21.76252943155062	27.648839556004035
45	27.17793474604776	22.334342415068953	22.53615876219307	27.95156407669021
46	28.119744365960308	21.493440968718467	22.166162125798856	28.220652539522366
47	27.413387151025898	21.627985200134546	22.43525058863101	28.523377060208542
48	26.370669357551296	23.14160780356542	22.838883282879248	27.648839556004035
49	28.75882946518668	23.37706020854356	21.8970736629667	25.967036663303062
50	26.101580894719138	23.94887319206189	21.96434577867474	27.98520013454423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.5
14	1.0
15	1.0
16	1.0
17	1.5
18	2.0
19	3.5
20	5.0
21	4.5
22	4.0
23	6.5
24	9.0
25	13.5
26	18.0
27	18.0
28	18.0
29	29.0
30	40.0
31	45.0
32	50.0
33	55.0
34	60.0
35	73.0
36	86.0
37	84.0
38	82.0
39	96.5
40	111.0
41	113.5
42	116.0
43	121.5
44	127.0
45	119.5
46	112.0
47	123.5
48	135.0
49	141.5
50	148.0
51	149.0
52	150.0
53	145.5
54	141.0
55	149.5
56	158.0
57	162.5
58	167.0
59	167.5
60	168.0
61	182.5
62	197.0
63	179.0
64	161.0
65	169.5
66	178.0
67	166.0
68	154.0
69	141.5
70	129.0
71	113.5
72	98.0
73	78.5
74	59.0
75	51.0
76	43.0
77	29.5
78	16.0
79	12.5
80	9.0
81	9.0
82	9.0
83	5.5
84	2.0
85	3.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03363605785401951
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.49276824756139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.42092286016202	91.12008072653886
2	4.438182458612188	8.476286579212916
3	0.14089468122578372	0.4036326942482341
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCGACCGGCGCGATCCGGCCACGCGGGCGGCGGGCAATCCGGCGGG	3	0.10090817356205853	No Hit
GCCTAGGGCGGCAGGTCGTCAGGGCGGCCGGGCGGGAGTTTGAATTCGCC	3	0.10090817356205853	No Hit
CAGGTGTTGAATCCATACATGGCGAATCGCCACCGCAATTGACCACAAAA	3	0.10090817356205853	No Hit
GGGGCTGGCCGTCGCCGGGGCCGCCGTCGCCCGGGGTGCCGCCATAGATC	3	0.10090817356205853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.03363605785401951	0.0
37	0.0	0.0	0.0	0.03363605785401951	0.0
38	0.0	0.0	0.0	0.03363605785401951	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE