FastQCFastQC Report
Thu 2 Feb 2017
SRR4061956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471609
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22680.15411702429109908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT855.638867E-1116.7563631
GGTATCA7000.015.1924351
CGAACGA456.756143E-414.77947216
GTCCTAA1800.013.1878791
CGTTATT1102.759407E-912.95032
GTTTTCG1155.313268E-912.39272515
GTATTAG1001.4515899E-712.3438541
GTATCAA18000.012.1855991
TTTTCGG1209.991709E-911.87636116
TTAGACC658.028993E-411.6912614
TGCCGTC907.4529835E-611.6120477
GTATTAT1157.120252E-811.5594621
GATAATT1001.9272848E-611.4009197
GCACCGT855.317801E-511.1773726
ACGGTAT1701.0913936E-1111.1769919
CTAGACA1701.0913936E-1111.175474
CTATTAG600.005897209511.0778181
CGCGGTT2150.011.04815410
CGCGCGT1553.1286618E-1011.0331487
CGGTCCA2000.010.92662310
Produced by FastQC (version 0.11.5)