##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061956_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1471609
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23730692052033	32.0	32.0	32.0	32.0	32.0
2	30.77457259367128	32.0	32.0	32.0	32.0	32.0
3	30.807323140861467	32.0	32.0	32.0	32.0	32.0
4	30.793352718011374	32.0	32.0	32.0	32.0	32.0
5	30.718824769351098	32.0	32.0	32.0	32.0	32.0
6	34.383697707747096	36.0	36.0	36.0	32.0	36.0
7	34.306705109849155	36.0	36.0	36.0	32.0	36.0
8	34.266499457396634	36.0	36.0	36.0	32.0	36.0
9	34.40016675625115	36.0	36.0	36.0	32.0	36.0
10	34.07251042906098	36.0	36.0	36.0	32.0	36.0
11	34.379704799304704	36.0	36.0	36.0	32.0	36.0
12	34.208881571123854	36.0	36.0	36.0	32.0	36.0
13	34.2897909702917	36.0	36.0	36.0	32.0	36.0
14	34.19780729799832	36.0	36.0	36.0	32.0	36.0
15	34.14757452557031	36.0	36.0	36.0	32.0	36.0
16	34.15170809637614	36.0	36.0	36.0	32.0	36.0
17	34.092765129868056	36.0	36.0	36.0	32.0	36.0
18	34.08640406521025	36.0	36.0	36.0	32.0	36.0
19	34.09473100531459	36.0	36.0	36.0	32.0	36.0
20	34.07050038427327	36.0	36.0	36.0	32.0	36.0
21	34.05377379453374	36.0	36.0	36.0	32.0	36.0
22	34.030357248426725	36.0	36.0	36.0	32.0	36.0
23	33.99745924358984	36.0	36.0	36.0	32.0	36.0
24	33.96194573422696	36.0	36.0	36.0	32.0	36.0
25	33.60503163544121	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	26.0
5	111.0
6	346.0
7	64.0
8	262.0
9	195.0
10	113.0
11	35.0
12	67.0
13	71.0
14	263.0
15	336.0
16	512.0
17	780.0
18	1069.0
19	1448.0
20	2109.0
21	2943.0
22	4583.0
23	6552.0
24	9134.0
25	12799.0
26	17491.0
27	22347.0
28	29612.0
29	39094.0
30	51386.0
31	68583.0
32	97075.0
33	137864.0
34	302314.0
35	662024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78840277169027	17.741318421601626	11.937335934596046	25.53294287211206
2	16.40402879027567	19.543859506064116	37.81584498045675	26.23626672320346
3	19.128188784611066	23.451229713448534	28.309877052438736	29.110704449501668
4	12.858793307327415	15.528318812607885	35.45621355660364	36.156674323461054
5	15.003528034619604	36.3940223265451	33.19370745455361	15.408742184281687
6	34.397981269213744	35.15248938037993	16.646845189160814	13.80268416124551
7	30.30986605351057	30.467962367090934	20.392315593983632	18.829855985414866
8	27.968341500350014	33.49408852216115	19.059864470382067	19.47770550710677
9	26.96914196763159	14.71750436105449	18.835060973120665	39.47829269819326
10	16.31794437001013	26.962179581832647	31.018921436605666	25.70095461155156
11	36.60951434705828	21.852578999840077	22.029309986968045	19.50859666613359
12	24.240729286384536	23.854025684522533	28.992155013503666	22.913090015589265
13	28.930400796912508	20.308340908719767	25.27523148022144	25.486026814146285
14	23.374874817669212	19.544635664990313	25.292953867589752	31.787535649750726
15	25.13680798161105	27.258913783412652	22.03482904616155	25.569449188814747
16	25.593728760168755	26.45832383671209	23.392236197468943	24.555711205650212
17	23.691485358965096	26.494772201050665	25.249993366169093	24.563749073815146
18	24.282381690689892	26.032980542174045	25.797249739267446	23.88738802786862
19	25.322223854768666	25.24032444139702	25.367935155255168	24.069516548579138
20	25.64228136654539	24.986787413046894	24.892173727323495	24.47875749308422
21	26.121772962090063	24.791473239944363	24.615034060107675	24.471719737857903
22	25.47374464556479	24.907162579598012	25.17048821020882	24.448604564628383
23	24.4100164583305	25.022035120173015	25.503475292101363	25.06447312939512
24	24.556925002380662	25.062305295950154	25.42253329524276	24.958236406426423
25	24.724229822764354	24.93263032172847	25.246659402576167	25.096480452931008
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	349.0
2	434.5
3	520.0
4	520.0
5	520.0
6	961.0
7	1402.0
8	1402.0
9	1402.0
10	1722.0
11	2042.0
12	2042.0
13	2042.0
14	2891.0
15	3740.0
16	3740.0
17	3740.0
18	6197.0
19	8654.0
20	8654.0
21	8654.0
22	14193.5
23	19733.0
24	19733.0
25	19733.0
26	29114.5
27	38496.0
28	38496.0
29	38496.0
30	50562.0
31	62628.0
32	62628.0
33	62628.0
34	78643.5
35	94659.0
36	94659.0
37	94659.0
38	112441.0
39	130223.0
40	130223.0
41	130223.0
42	146808.5
43	163394.0
44	163394.0
45	163394.0
46	177690.5
47	191987.0
48	191987.0
49	191987.0
50	197018.5
51	202050.0
52	202050.0
53	202050.0
54	189139.0
55	176228.0
56	176228.0
57	176228.0
58	159536.0
59	142844.0
60	142844.0
61	142844.0
62	123460.0
63	104076.0
64	104076.0
65	104076.0
66	84530.5
67	64985.0
68	64985.0
69	64985.0
70	48935.5
71	32886.0
72	32886.0
73	32886.0
74	24428.0
75	15970.0
76	15970.0
77	15970.0
78	12327.5
79	8685.0
80	8685.0
81	8685.0
82	6030.5
83	3376.0
84	3376.0
85	3376.0
86	2407.5
87	1439.0
88	1439.0
89	1439.0
90	960.0
91	481.0
92	481.0
93	481.0
94	320.5
95	160.0
96	160.0
97	160.0
98	381.0
99	602.0
100	602.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010872453212775947
2	6.795283257984967E-5
3	4.0771699547909805E-4
4	0.0101249720543976
5	0.03635476543021957
6	0.06672968159341239
7	0.11001563594677663
8	0.11361713607350866
9	0.12102399482471227
10	0.13631338215517844
11	0.1456908730511977
12	0.13644928782033813
13	0.1316246367071688
14	0.11912131551247648
15	0.1380122029696747
16	0.1214996646527712
17	0.12707179692431889
18	0.11633524937670264
19	0.10281263569331255
20	0.09649302226338653
21	0.09608530526790744
22	0.10641413582004458
23	0.08351403124063525
24	0.0971045977566052
25	0.0926197108063351
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1471609.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.92703877623548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0718960406404	47.385445801379184
2	12.79024310627051	15.329627891739026
3	3.6821201091451004	6.619756636784844
4	1.4958104380732127	3.5855796049724478
5	0.7615303918564047	2.281813066103028
6	0.4694165565682453	1.6878446512603302
7	0.3107655847107595	1.3036282871696825
8	0.2307863138341847	1.106427230253251
9	0.1612217125162388	0.8695385835778555
>10	0.9088424352077052	9.839748474308147
>50	0.06475809293364063	2.7039409764502986
>100	0.04907158672104724	5.719560089115897
>500	0.0033093972307228054	1.323220571221447
>1k	2.2823429177398657E-4	0.243868135664669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2268	0.15411702429109908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0385849773954902E-4
2	0.0	0.0	0.0	0.0	2.0385849773954902E-4
3	0.0	0.0	0.0	0.0	2.0385849773954902E-4
4	0.0	0.0	0.0	0.0	2.0385849773954902E-4
5	0.0	0.0	0.0	0.0	2.0385849773954902E-4
6	0.0	0.0	0.0	0.0	2.0385849773954902E-4
7	0.0	0.0	0.0	0.0	2.0385849773954902E-4
8	0.0	0.0	0.0	0.0	2.0385849773954902E-4
9	0.0	0.0	0.0	6.795283257984967E-5	2.0385849773954902E-4
10	0.0	0.0	0.0	6.795283257984967E-5	2.718113303193987E-4
11	0.0	0.0	0.0	6.795283257984967E-5	2.718113303193987E-4
12	0.0	0.0	0.0	6.795283257984967E-5	3.397641628992484E-4
13	0.0	0.0	0.0	6.795283257984967E-5	4.0771699547909805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	85	5.638867E-11	16.756363	1
GGTATCA	700	0.0	15.192435	1
CGAACGA	45	6.756143E-4	14.779472	16
GTCCTAA	180	0.0	13.187879	1
CGTTATT	110	2.759407E-9	12.9503	2
GTTTTCG	115	5.313268E-9	12.392725	15
GTATTAG	100	1.4515899E-7	12.343854	1
GTATCAA	1800	0.0	12.185599	1
TTTTCGG	120	9.991709E-9	11.876361	16
TTAGACC	65	8.028993E-4	11.691261	4
TGCCGTC	90	7.4529835E-6	11.612047	7
GTATTAT	115	7.120252E-8	11.559462	1
GATAATT	100	1.9272848E-6	11.400919	7
GCACCGT	85	5.317801E-5	11.177372	6
ACGGTAT	170	1.0913936E-11	11.176991	9
CTAGACA	170	1.0913936E-11	11.17547	4
CTATTAG	60	0.0058972095	11.077818	1
CGCGGTT	215	0.0	11.048154	10
CGCGCGT	155	3.1286618E-10	11.033148	7
CGGTCCA	200	0.0	10.926623	10
>>END_MODULE