FastQCFastQC Report
Thu 2 Feb 2017
SRR4061956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471609
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17250.028.0687241
GGTATCA8500.024.0798111
ATAAGAC1851.8189894E-1219.025913
CCTAGAC1557.221388E-1018.4505313
ACCGTAT600.007415359418.3322568
AATCGTA750.001291559517.59896713
ATCAACG27300.017.5667363
TCAACGC27800.017.1716544
GTAGGAC4950.016.8878973
AACGCAG28200.016.8500756
GCCGTTT1702.4701876E-916.82254215
CAACGCA28400.016.8088725
TATCAAC29550.016.4525032
AACCGAC1259.445192E-615.83960830
TACGAAT2550.015.53113941
TGTAGGA5100.015.5285012
GATATAC1857.530616E-915.4653831
CGTCTTA1004.946806E-415.399096515
TCTAGCG2601.8189894E-1215.23039328
GTATTAG2102.5302143E-914.6722871