##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4061956_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1471609 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28115756291243 32.0 32.0 32.0 32.0 32.0 2 31.44139985553228 32.0 32.0 32.0 32.0 32.0 3 31.56950589456846 32.0 32.0 32.0 32.0 32.0 4 31.648281574793305 32.0 32.0 32.0 32.0 32.0 5 31.628095506347133 32.0 32.0 32.0 32.0 32.0 6 35.26648722588677 36.0 36.0 36.0 36.0 36.0 7 35.26913602730073 36.0 36.0 36.0 36.0 36.0 8 35.22312380530426 36.0 36.0 36.0 36.0 36.0 9 35.303594908701974 36.0 36.0 36.0 36.0 36.0 10 35.195240039983446 36.0 36.0 36.0 36.0 36.0 11 35.30897813209895 36.0 36.0 36.0 36.0 36.0 12 35.235326095450624 36.0 36.0 36.0 36.0 36.0 13 35.26388735051226 36.0 36.0 36.0 36.0 36.0 14 35.22486747498826 36.0 36.0 36.0 36.0 36.0 15 35.20364988254353 36.0 36.0 36.0 36.0 36.0 16 35.2178146504948 36.0 36.0 36.0 36.0 36.0 17 35.2031592630923 36.0 36.0 36.0 36.0 36.0 18 35.193278241706864 36.0 36.0 36.0 36.0 36.0 19 35.186564501847975 36.0 36.0 36.0 36.0 36.0 20 35.189776632244026 36.0 36.0 36.0 36.0 36.0 21 35.17483244530307 36.0 36.0 36.0 36.0 36.0 22 35.164014354356354 36.0 36.0 36.0 36.0 36.0 23 35.13158386500762 36.0 36.0 36.0 36.0 36.0 24 35.10916146884125 36.0 36.0 36.0 36.0 36.0 25 35.10147396489149 36.0 36.0 36.0 36.0 36.0 26 35.052189814006304 36.0 36.0 36.0 36.0 36.0 27 35.04822748433857 36.0 36.0 36.0 36.0 36.0 28 35.02051903732581 36.0 36.0 36.0 36.0 36.0 29 34.9976794107674 36.0 36.0 36.0 36.0 36.0 30 34.98397196537939 36.0 36.0 36.0 36.0 36.0 31 34.97114790681492 36.0 36.0 36.0 36.0 36.0 32 34.9551273470059 36.0 36.0 36.0 36.0 36.0 33 34.93383772455863 36.0 36.0 36.0 36.0 36.0 34 34.9158519688314 36.0 36.0 36.0 32.0 36.0 35 34.906865206722706 36.0 36.0 36.0 36.0 36.0 36 34.87864031818234 36.0 36.0 36.0 32.0 36.0 37 34.87350784073759 36.0 36.0 36.0 32.0 36.0 38 34.86138845304697 36.0 36.0 36.0 32.0 36.0 39 34.833362666306066 36.0 36.0 36.0 32.0 36.0 40 34.82550867791648 36.0 36.0 36.0 32.0 36.0 41 34.795179969679445 36.0 36.0 36.0 32.0 36.0 42 34.775352012660974 36.0 36.0 36.0 32.0 36.0 43 34.76844053005927 36.0 36.0 36.0 32.0 36.0 44 34.70932156571481 36.0 36.0 36.0 32.0 36.0 45 34.70285449463818 36.0 36.0 36.0 32.0 36.0 46 34.678340510285004 36.0 36.0 36.0 32.0 36.0 47 34.65786020607376 36.0 36.0 36.0 32.0 36.0 48 34.638294546989044 36.0 36.0 36.0 32.0 36.0 49 34.608941641427855 36.0 36.0 36.0 32.0 36.0 50 34.21307018372407 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 5.0 20 9.0 21 26.0 22 89.0 23 295.0 24 688.0 25 1593.0 26 3441.0 27 6673.0 28 11434.0 29 18186.0 30 27276.0 31 40840.0 32 61910.0 33 104677.0 34 236977.0 35 957490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66145291650522 18.163087736471102 12.418785814792912 25.756673532230767 2 15.988727478571812 20.04664491625457 37.48779342499676 26.476834180176862 3 18.39795216647957 23.978393643942255 28.65709796929065 28.966556220287522 4 12.700112597843585 15.937521447612783 35.59817859227553 35.7641873622681 5 14.710914863197264 36.69489429250181 33.049290300134274 15.544900544166653 6 34.48180493718436 35.35615462813467 16.544691249300087 13.617349185380888 7 30.379061285980175 30.477796751718696 20.355882574787188 18.787259387513938 8 27.9977901738845 33.554633058101714 19.001106951642726 19.44646981637106 9 27.094697028898302 14.473613575345082 18.63647205201925 39.79521734373736 10 16.279596006819748 26.971022873602973 31.09983698115464 25.649544138422637 11 36.86529506139198 21.782280483470814 21.995108755110905 19.357315700026298 12 24.247761632173788 23.682121869410878 29.131806839864964 22.938309658550374 13 29.169908583054333 20.14257863331904 25.342397335161714 25.34511544846491 14 23.376657794291827 19.35962609633401 25.310935173677247 31.95278093569691 15 25.070586004842323 27.197781475921936 22.149293732234582 25.58233878700117 16 25.713019836327028 26.331125521798487 23.354215465825128 24.601639176049357 17 23.805304262205517 26.410004287823735 25.252359831993417 24.53233161797733 18 24.47744236236824 25.807280199618376 25.885426010187494 23.829851427825886 19 25.52792591505347 25.028132491804882 25.40058222026518 24.043359372876473 20 25.769057750512026 24.85665320744819 24.91482095071643 24.45946809132336 21 26.33124695486369 24.581053798937084 24.66803342463929 24.41966582155994 22 25.705673178133594 24.705135671227886 25.22742114243661 24.36177000820191 23 24.55110328293948 24.8229147979625 25.575153165788716 25.050828753309307 24 24.65369161174319 24.94922169376995 25.461319807854483 24.935766886632376 25 24.97810214018028 24.625833698580802 25.320789206342738 25.075274954896187 26 24.693394944278335 25.377548246806196 25.429532481652622 24.499524327262844 27 24.801933135317434 25.19232481131069 25.237173985576234 24.768568067795645 28 24.63337150079676 25.178837783750176 25.450857408848282 24.736933306604783 29 24.563570856794158 25.059764800537103 25.38872602782546 24.98793831484328 30 24.770690205831787 25.19934220808785 25.38315699345606 24.646810592624306 31 25.259376843226843 25.03628693588764 24.662680992984527 25.041655227900982 32 24.688196097031934 25.177392195422936 25.06085221643411 25.073559491111023 33 24.540416755855002 24.986868079409895 25.485167555152366 24.987547609582734 34 24.96790901313335 24.784910542764084 25.65281119763087 24.59436924647169 35 25.35169060196819 24.77979839712532 25.306773688065963 24.561737312840528 36 24.806300055857342 25.323829883814007 24.989025595166098 24.88084446516255 37 25.26005990823494 25.034520625394137 25.088272229108227 24.617147237262703 38 24.781106938052158 25.05740315532183 25.378616194926778 24.782873711699235 39 25.24746924599602 24.692833703792548 25.11285358695349 24.94684346325794 40 25.45932810820288 24.92630500283364 25.17331405281033 24.441052836153155 41 24.886415682586367 25.37241929499577 25.212456696616048 24.52870832580182 42 25.245169493310716 25.37394676164245 25.232801439043502 24.14808230600333 43 25.017753425476723 24.81871119305058 25.365074985712315 24.79846039576038 44 24.71611488869529 24.93656331504418 25.164962563181852 25.18235923307868 45 24.973259924407472 25.295567874942073 24.817440373368925 24.913731827281538 46 25.070995458601104 25.1100692931985 24.91633102809035 24.902604220110046 47 24.90598604230798 25.08728654040867 25.431336173798407 24.575391243484937 48 25.146019602473313 25.634416522997196 24.709676674035318 24.509887200494173 49 24.897029992287017 25.266301744085297 24.944055397558348 24.892612866069335 50 25.080627295992215 25.622695513547967 24.620375567584905 24.67630162287491 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 112.0 1 86.0 2 60.0 3 126.0 4 192.0 5 197.0 6 202.0 7 164.5 8 127.0 9 175.5 10 224.0 11 427.0 12 630.0 13 1126.0 14 1622.0 15 2031.0 16 2440.0 17 2434.0 18 2428.0 19 2322.0 20 2216.0 21 2584.5 22 2953.0 23 3752.0 24 4551.0 25 5903.0 26 7255.0 27 9636.0 28 12017.0 29 14460.0 30 16903.0 31 20316.5 32 23730.0 33 27586.0 34 31442.0 35 37029.5 36 42617.0 37 50123.5 38 57630.0 39 63898.0 40 70166.0 41 76128.5 42 82091.0 43 85159.5 44 88228.0 45 95032.5 46 101837.0 47 106238.5 48 110640.0 49 113262.5 50 115885.0 51 114192.5 52 112500.0 53 110414.5 54 108329.0 55 107367.0 56 106405.0 57 100808.0 58 95211.0 59 87643.5 60 80076.0 61 69967.5 62 59859.0 63 51438.5 64 43018.0 65 35894.0 66 28770.0 67 24680.0 68 20590.0 69 17795.5 70 15001.0 71 11830.5 72 8660.0 73 7400.5 74 6141.0 75 4671.5 76 3202.0 77 2749.0 78 2296.0 79 1890.0 80 1484.0 81 1155.0 82 826.0 83 690.0 84 554.0 85 421.5 86 289.0 87 194.5 88 100.0 89 67.0 90 34.0 91 24.0 92 14.0 93 16.5 94 19.0 95 16.5 96 14.0 97 12.5 98 11.0 99 9.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03390846345734499 2 0.004484886950270078 3 3.397641628992484E-4 4 0.0 5 6.795283257984967E-5 6 6.795283257984967E-5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 6.795283257984967E-5 13 0.0 14 0.0 15 0.0 16 0.0010872453212775947 17 0.0 18 6.795283257984967E-5 19 6.795283257984967E-5 20 2.0385849773954902E-4 21 0.0 22 0.0 23 6.795283257984967E-5 24 0.0010872453212775947 25 2.718113303193987E-4 26 6.115754932186471E-4 27 6.795283257984968E-4 28 0.0016308679819163922 29 4.7566982805894776E-4 30 0.0012911038190171439 31 3.397641628992484E-4 32 7.474811583783464E-4 33 2.718113303193987E-4 34 8.154339909581961E-4 35 2.0385849773954902E-4 36 2.0385849773954902E-4 37 0.0016988208144962417 38 0.0 39 8.154339909581961E-4 40 2.0385849773954902E-4 41 0.0010192924886977452 42 0.0051644152760685754 43 0.0042130756199506795 44 0.00428102845253053 45 0.0023783491402947386 46 0.0024463019728745883 47 0.0012911038190171439 48 0.004077169954790981 49 0.004348981285110379 50 0.0015629151493365425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1471609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.662294450292435 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0846714037243 48.765422946010695 2 12.891917948645974 15.898904811568359 3 3.6864395575653695 6.819429644154047 4 1.4458970204299382 3.5662931127420543 5 0.7795320008450551 2.4033865884766707 6 0.4653187282387635 1.7215572260336547 7 0.3155132477649718 1.3618689550656236 8 0.22428447212792021 1.1063916128784177 9 0.16065221632884696 0.8915565840625297 >10 0.8481153664650071 9.273297164868536 >50 0.05562778240813085 2.3627435730118083 >100 0.03903610109673423 4.584560517829504 >500 0.0029941543590685155 1.2445872632979964 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.718113303193987E-4 13 0.0 0.0 0.0 0.0 5.436226606387974E-4 14 0.0 0.0 0.0 0.0 5.436226606387974E-4 15 0.0 0.0 0.0 0.0 6.795283257984968E-4 16 0.0 0.0 0.0 6.795283257984967E-5 8.154339909581961E-4 17 0.0 0.0 0.0 6.795283257984967E-5 8.154339909581961E-4 18 0.0 0.0 0.0 2.718113303193987E-4 8.833868235380458E-4 19 0.0 0.0 0.0 2.718113303193987E-4 8.833868235380458E-4 20 0.0 0.0 0.0 3.397641628992484E-4 8.833868235380458E-4 21 0.0 0.0 0.0 3.397641628992484E-4 8.833868235380458E-4 22 0.0 0.0 0.0 3.397641628992484E-4 8.833868235380458E-4 23 0.0 0.0 0.0 6.795283257984968E-4 8.833868235380458E-4 24 0.0 0.0 0.0 9.513396561178954E-4 8.833868235380458E-4 25 0.0 0.0 0.0 0.0012911038190171439 8.833868235380458E-4 26 0.0 0.0 0.0 0.0015629151493365425 0.001019292488697745 27 0.0 0.0 0.0 0.0017667736470760916 0.0010872453212775947 28 0.0 0.0 0.0 0.0019706321448156408 0.0010872453212775947 29 0.0 0.0 0.0 0.0031937831312529347 0.0010872453212775947 30 0.0 0.0 0.0 0.00428102845253053 0.0010872453212775947 31 0.0 0.0 0.0 0.007406858751203615 0.0010872453212775947 32 0.0 0.0 0.0 0.011144264543095347 0.0010872453212775947 33 0.0 0.0 0.0 0.014202142009188581 0.0010872453212775947 34 0.0 0.0 0.0 0.018007500633660165 0.0010872453212775947 35 0.0 0.0 0.0 0.02283215174682949 0.0010872453212775947 36 0.0 0.0 0.0 0.028336331185797314 0.0010872453212775947 37 0.0 0.0 0.0 0.03519956727636213 0.0010872453212775947 38 0.0 0.0 0.0 0.04627587898687763 0.0011551981538574444 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1725 0.0 28.068724 1 GGTATCA 850 0.0 24.079811 1 ATAAGAC 185 1.8189894E-12 19.02591 3 CCTAGAC 155 7.221388E-10 18.450531 3 ACCGTAT 60 0.0074153594 18.332256 8 AATCGTA 75 0.0012915595 17.598967 13 ATCAACG 2730 0.0 17.566736 3 TCAACGC 2780 0.0 17.171654 4 GTAGGAC 495 0.0 16.887897 3 AACGCAG 2820 0.0 16.850075 6 GCCGTTT 170 2.4701876E-9 16.822542 15 CAACGCA 2840 0.0 16.808872 5 TATCAAC 2955 0.0 16.452503 2 AACCGAC 125 9.445192E-6 15.839608 30 TACGAAT 255 0.0 15.531139 41 TGTAGGA 510 0.0 15.528501 2 GATATAC 185 7.530616E-9 15.465383 1 CGTCTTA 100 4.946806E-4 15.3990965 15 TCTAGCG 260 1.8189894E-12 15.230393 28 GTATTAG 210 2.5302143E-9 14.672287 1 >>END_MODULE