FastQCFastQC Report
Thu 2 Feb 2017
SRR4061955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530319
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32140.12701955761309147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT902.2864697E-914.779217
GGTATCA11150.013.7963811
GTCTAGC1405.2750693E-1112.2087031
GTATCAA26150.012.0919861
TAGGACC7200.012.006924
GTAGGAC11700.011.5282943
GGCGAAT752.0733093E-411.40087910
TGTAGGA12900.011.1913392
TTACCGC951.3604522E-511.00041216
TGGCGAG8950.010.72043618
CCGATAA803.7697502E-410.6883239
TCGCGTA803.7697502E-410.6883239
AGGACGT9050.010.4980475
TAGGACG8950.010.4022134
CTGTAGG12450.010.1439551
ATTACCG750.00264772810.13371315
GTATACG750.002658615410.1287021
TACACCG2002.5465852E-119.9757695
GGACGTG9250.09.9631276
GGAATAT15450.09.83914212
Produced by FastQC (version 0.11.5)