##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061955_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2530319
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231855746251757	32.0	32.0	32.0	32.0	32.0
2	30.717256994078614	32.0	32.0	32.0	32.0	32.0
3	30.766665784037507	32.0	32.0	32.0	32.0	32.0
4	30.767446713240503	32.0	32.0	32.0	32.0	32.0
5	30.659251659573357	32.0	32.0	32.0	32.0	32.0
6	34.31706160369503	36.0	36.0	36.0	32.0	36.0
7	34.2658747770538	36.0	36.0	36.0	32.0	36.0
8	34.205696593986765	36.0	36.0	36.0	32.0	36.0
9	34.36061540066687	36.0	36.0	36.0	32.0	36.0
10	34.01534312472064	36.0	36.0	36.0	32.0	36.0
11	34.34058274865738	36.0	36.0	36.0	32.0	36.0
12	34.165266909034	36.0	36.0	36.0	32.0	36.0
13	34.237969995087575	36.0	36.0	36.0	32.0	36.0
14	34.15216105163025	36.0	36.0	36.0	32.0	36.0
15	34.086917499335065	36.0	36.0	36.0	32.0	36.0
16	34.09560691754676	36.0	36.0	36.0	32.0	36.0
17	34.03409688659809	36.0	36.0	36.0	32.0	36.0
18	34.03450908758935	36.0	36.0	36.0	32.0	36.0
19	34.02917260629984	36.0	36.0	36.0	32.0	36.0
20	34.01049709542552	36.0	36.0	36.0	32.0	36.0
21	33.996849409106126	36.0	36.0	36.0	32.0	36.0
22	33.967380002284294	36.0	36.0	36.0	32.0	36.0
23	33.93325110391219	36.0	36.0	36.0	32.0	36.0
24	33.89882619543228	36.0	36.0	36.0	32.0	36.0
25	33.5222120214882	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	43.0
5	210.0
6	534.0
7	113.0
8	444.0
9	348.0
10	199.0
11	53.0
12	110.0
13	107.0
14	259.0
15	403.0
16	686.0
17	1075.0
18	1504.0
19	2213.0
20	3331.0
21	4973.0
22	7634.0
23	11294.0
24	16143.0
25	22966.0
26	31039.0
27	40385.0
28	53665.0
29	70701.0
30	92826.0
31	125621.0
32	176170.0
33	245429.0
34	525625.0
35	1094215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.867841757894325	17.521321582421056	12.006520965893372	25.604315693791253
2	16.46810121739735	19.016320491195565	38.596720725790775	25.91885756561631
3	19.98799351381003	22.23278750851184	27.66534705977436	30.113871917903772
4	13.354956162783068	15.647763950163673	34.306268433488455	36.691011453564805
5	15.21250452667218	35.899831267800295	33.321288563688896	15.56637564183863
6	35.32415886056101	33.89586595077358	16.51456509178889	14.265410096876515
7	31.31017566070581	29.512066782718783	19.51091944927995	19.666838107295458
8	27.852842486497646	34.49606591117074	18.323868862914402	19.32722273941721
9	26.567337805835884	15.639131957290303	18.808291078010285	38.98523915886353
10	17.264329624701908	27.02372632373764	30.48112745350228	25.23081659805817
11	36.12753319882568	21.747338276186053	22.071549732343495	20.053578792644768
12	24.67067967788798	23.539987186118267	28.58124843932223	23.208084696671516
13	30.120611132282065	19.98379927243142	24.47920438361455	25.416385211671965
14	23.112125792335025	19.868082648159724	25.004391969422397	32.01539959008286
15	24.93500953140774	27.74183795300899	21.76690969085155	25.55624282473172
16	25.234017246270778	26.121575406832115	24.01107756152783	24.63332978536928
17	23.870327457833156	26.670410082054396	25.100391594489185	24.35887086562326
18	24.318931592853758	25.73309762580586	26.10873671474457	23.839234066595818
19	25.609588691559615	25.28765006561013	25.07655833446409	24.02620290836617
20	25.709109249681063	25.099439263427513	25.139116073459455	24.052335413431965
21	25.57291345260756	24.811547352881142	24.693902900208705	24.92163629430259
22	25.816691709215263	24.98478853090836	24.886517297556907	24.31200246231947
23	24.64727841727586	25.300075389664894	25.265584420207592	24.78706177285165
24	24.794279950157243	25.038707252912438	25.48859748017168	24.678415316758638
25	24.7500916722804	25.07188412149079	25.401231869754554	24.776792336474255
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	115.0
2	378.5
3	642.0
4	642.0
5	642.0
6	1280.5
7	1919.0
8	1919.0
9	1919.0
10	2379.0
11	2839.0
12	2839.0
13	2839.0
14	3980.5
15	5122.0
16	5122.0
17	5122.0
18	9729.0
19	14336.0
20	14336.0
21	14336.0
22	22707.5
23	31079.0
24	31079.0
25	31079.0
26	47523.0
27	63967.0
28	63967.0
29	63967.0
30	85662.5
31	107358.0
32	107358.0
33	107358.0
34	131926.5
35	156495.0
36	156495.0
37	156495.0
38	185309.5
39	214124.0
40	214124.0
41	214124.0
42	244429.5
43	274735.0
44	274735.0
45	274735.0
46	301816.5
47	328898.0
48	328898.0
49	328898.0
50	340090.5
51	351283.0
52	351283.0
53	351283.0
54	332440.5
55	313598.0
56	313598.0
57	313598.0
58	285836.5
59	258075.0
60	258075.0
61	258075.0
62	222318.5
63	186562.0
64	186562.0
65	186562.0
66	150971.5
67	115381.0
68	115381.0
69	115381.0
70	86582.5
71	57784.0
72	57784.0
73	57784.0
74	42032.0
75	26280.0
76	26280.0
77	26280.0
78	18984.0
79	11688.0
80	11688.0
81	11688.0
82	8174.5
83	4661.0
84	4661.0
85	4661.0
86	3206.0
87	1751.0
88	1751.0
89	1751.0
90	1124.0
91	497.0
92	497.0
93	497.0
94	350.5
95	204.0
96	204.0
97	204.0
98	565.0
99	926.0
100	926.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.508934644208892E-4
2	1.1856212596119304E-4
3	7.904141730746203E-4
4	0.01094723629708349
5	0.034264454402784784
6	0.06465587935750393
7	0.10745680682949463
8	0.1104999013958319
9	0.11951062296888257
10	0.1352003443044138
11	0.14120749201978092
12	0.13326382958038097
13	0.12931175871500786
14	0.11773219107946468
15	0.13476561650922275
16	0.12045911997657212
17	0.1270590783217452
18	0.11433341013524381
19	0.09911793730355738
20	0.09461257651703205
21	0.09338743454876637
22	0.10358377738142897
23	0.08382342305456347
24	0.09362455880068878
25	0.09058146423435148
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2530319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.88115911666058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85854355255181	29.109266132569378
2	16.286700999184482	14.619320380597475
3	7.30745970533919	9.839017853217417
4	3.6948982035772118	6.633252567784487
5	2.2363860773564435	5.0185799692059465
6	1.3401097148082126	3.6087406406454012
7	0.9143492363038191	2.872593749392415
8	0.6509886888782488	2.3373701542952747
9	0.46209965266892256	1.866561123526875
>10	2.1028350996546563	15.957740187827941
>50	0.09758699216529099	3.0158797565120348
>100	0.04476790907750285	3.7156188785469975
>500	0.0023892580466207385	0.728864433384181
>1k	8.849103876355823E-4	0.6771941724942765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3214	0.12701955761309147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9520708653731014E-5
2	0.0	0.0	0.0	0.0	3.9520708653731014E-5
3	0.0	0.0	0.0	0.0	3.9520708653731014E-5
4	0.0	0.0	0.0	0.0	3.9520708653731014E-5
5	0.0	0.0	0.0	0.0	3.9520708653731014E-5
6	0.0	0.0	0.0	0.0	7.904141730746203E-5
7	0.0	0.0	0.0	3.9520708653731014E-5	7.904141730746203E-5
8	0.0	0.0	0.0	3.9520708653731014E-5	1.9760354326865505E-4
9	0.0	0.0	0.0	3.9520708653731014E-5	1.9760354326865505E-4
10	0.0	0.0	0.0	3.9520708653731014E-5	1.9760354326865505E-4
11	0.0	0.0	0.0	3.9520708653731014E-5	1.9760354326865505E-4
12	0.0	0.0	0.0	3.9520708653731014E-5	3.556863778835791E-4
13	0.0	0.0	0.0	3.9520708653731014E-5	3.556863778835791E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCT	90	2.2864697E-9	14.77921	7
GGTATCA	1115	0.0	13.796381	1
GTCTAGC	140	5.2750693E-11	12.208703	1
GTATCAA	2615	0.0	12.091986	1
TAGGACC	720	0.0	12.00692	4
GTAGGAC	1170	0.0	11.528294	3
GGCGAAT	75	2.0733093E-4	11.400879	10
TGTAGGA	1290	0.0	11.191339	2
TTACCGC	95	1.3604522E-5	11.000412	16
TGGCGAG	895	0.0	10.720436	18
CCGATAA	80	3.7697502E-4	10.688323	9
TCGCGTA	80	3.7697502E-4	10.688323	9
AGGACGT	905	0.0	10.498047	5
TAGGACG	895	0.0	10.402213	4
CTGTAGG	1245	0.0	10.143955	1
ATTACCG	75	0.002647728	10.133713	15
GTATACG	75	0.0026586154	10.128702	1
TACACCG	200	2.5465852E-11	9.975769	5
GGACGTG	925	0.0	9.963127	6
GGAATAT	1545	0.0	9.839142	12
>>END_MODULE