FastQCFastQC Report
Thu 2 Feb 2017
SRR4061955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530319
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25350.026.7396681
GGTATCA13650.022.249981
GTAGGAC14900.021.5564943
TAGGACC10650.021.2764514
TAGGACG10400.019.2495084
TGTAGGA17400.018.7132742
CTGTAGG17600.018.3817961
GTCCTAC14650.017.8769151
ATTTCGT6150.017.52939242
GGACGTG11550.017.5233616
AGGACGT11450.017.2921335
TATCAAC39100.017.1617032
ATCAACG39950.016.7404963
GTCCTAA6250.016.5501171
GATTTCG6800.016.50055541
TCAACGC40950.016.2779714
TTAGGAC8000.015.9495913
ATATGGC25800.015.94532815
CAACGCA41950.015.9423815
CGGATCG2353.6379788E-1215.91480126
Produced by FastQC (version 0.11.5)