##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2530319
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23994168324231	32.0	32.0	32.0	32.0	32.0
2	31.390303357007554	32.0	32.0	32.0	32.0	32.0
3	31.540021633635916	32.0	32.0	32.0	32.0	32.0
4	31.6247809070714	32.0	32.0	32.0	32.0	32.0
5	31.595285811788948	32.0	32.0	32.0	32.0	32.0
6	35.234194581789886	36.0	36.0	36.0	36.0	36.0
7	35.23933227391487	36.0	36.0	36.0	36.0	36.0
8	35.18663931306685	36.0	36.0	36.0	36.0	36.0
9	35.28590782427038	36.0	36.0	36.0	36.0	36.0
10	35.153962405530685	36.0	36.0	36.0	36.0	36.0
11	35.27791476094516	36.0	36.0	36.0	36.0	36.0
12	35.206461319699216	36.0	36.0	36.0	36.0	36.0
13	35.237960114910415	36.0	36.0	36.0	36.0	36.0
14	35.20337751880297	36.0	36.0	36.0	36.0	36.0
15	35.178275940701546	36.0	36.0	36.0	36.0	36.0
16	35.1944142220803	36.0	36.0	36.0	36.0	36.0
17	35.17623390568541	36.0	36.0	36.0	36.0	36.0
18	35.17222334417123	36.0	36.0	36.0	36.0	36.0
19	35.16160650099849	36.0	36.0	36.0	36.0	36.0
20	35.16113383332299	36.0	36.0	36.0	36.0	36.0
21	35.144998318393846	36.0	36.0	36.0	36.0	36.0
22	35.12603351593218	36.0	36.0	36.0	36.0	36.0
23	35.10071180748356	36.0	36.0	36.0	36.0	36.0
24	35.087028552526384	36.0	36.0	36.0	36.0	36.0
25	35.06664653745239	36.0	36.0	36.0	36.0	36.0
26	35.02372862868279	36.0	36.0	36.0	36.0	36.0
27	35.01203603182049	36.0	36.0	36.0	36.0	36.0
28	34.98864490998961	36.0	36.0	36.0	36.0	36.0
29	34.96580628766571	36.0	36.0	36.0	36.0	36.0
30	34.93681310538315	36.0	36.0	36.0	36.0	36.0
31	34.93458216137965	36.0	36.0	36.0	36.0	36.0
32	34.90850758343118	36.0	36.0	36.0	32.0	36.0
33	34.90232970625443	36.0	36.0	36.0	32.0	36.0
34	34.88727666353531	36.0	36.0	36.0	32.0	36.0
35	34.88146988581282	36.0	36.0	36.0	32.0	36.0
36	34.84241473110703	36.0	36.0	36.0	32.0	36.0
37	34.82380047733112	36.0	36.0	36.0	32.0	36.0
38	34.81547741608864	36.0	36.0	36.0	32.0	36.0
39	34.76970690256841	36.0	36.0	36.0	32.0	36.0
40	34.77384551117863	36.0	36.0	36.0	32.0	36.0
41	34.756255634171026	36.0	36.0	36.0	32.0	36.0
42	34.73841835752725	36.0	36.0	36.0	32.0	36.0
43	34.73518319231686	36.0	36.0	36.0	32.0	36.0
44	34.68525035776121	36.0	36.0	36.0	32.0	36.0
45	34.667621355252045	36.0	36.0	36.0	32.0	36.0
46	34.63499266298044	36.0	36.0	36.0	32.0	36.0
47	34.61487899351821	36.0	36.0	36.0	32.0	36.0
48	34.59548539136765	36.0	36.0	36.0	32.0	36.0
49	34.56720200101252	36.0	36.0	36.0	32.0	36.0
50	34.16158357898747	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	19.0
21	51.0
22	175.0
23	534.0
24	1187.0
25	2862.0
26	6144.0
27	11648.0
28	19862.0
29	31965.0
30	48440.0
31	72320.0
32	111087.0
33	189515.0
34	430567.0
35	1603936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5603279817484	18.0357939871067	12.485792820882606	25.918085210262298
2	15.95204651644414	19.434890969179534	38.279868563853796	26.333193950522528
3	19.32942683375535	22.699326130798838	27.947530601945292	30.023716433500518
4	13.181228604467895	15.99921432800146	34.38267443064469	36.43688263688596
5	14.910813581534022	36.196517591859994	33.1310530929314	15.76161573367458
6	35.41420732201327	34.07216097600496	16.406534364298516	14.107097337683253
7	31.44409855041993	29.4577086920661	19.528446808485413	19.56974594902856
8	27.87660370095628	34.5420478603686	18.243905215113195	19.33744322356193
9	26.650118028596392	15.435445096053108	18.772059965561656	39.142376909788844
10	17.17659658975266	27.077024006892426	30.540545347120812	25.205834056234107
11	36.37956320922382	21.5843140726525	22.101284462551956	19.93483825557173
12	24.718385310310676	23.393967321906842	28.597145261131107	23.290502106651374
13	30.177341275941888	19.82248088086917	24.636498401980145	25.3636794412088
14	23.12933665676146	19.68372367278592	25.059172381031797	32.127767289420824
15	25.013091234741548	27.72456753476538	21.747574120101063	25.51476711039201
16	25.384203150453978	26.023494428728274	24.02025528968479	24.572047131132962
17	24.0370087724117	26.581628640499478	25.12991444952198	24.251448137566843
18	24.504784781651605	25.616464992091892	26.118418504580852	23.76033172167565
19	25.769833764043188	25.205715168719834	25.033879127493414	23.990571939743567
20	25.852632344403514	25.002183526056676	25.14102021451118	24.004163915028627
21	25.73676510225197	24.693694626525204	24.70851489812743	24.861025373095398
22	25.933647101412905	24.85552216933912	24.93049295365525	24.280337775592724
23	24.776648626773394	25.13158425146563	25.298361707896007	24.793405413864978
24	24.934750820060863	24.93111488756274	25.493064063549774	24.64107022882662
25	24.903579198637324	24.884648749266397	25.47034657740242	24.741425474693862
26	24.344358214240405	25.565040131493927	25.620566990487713	24.470034663777955
27	24.80129944295473	25.439046126668025	25.108099189231382	24.651555241145864
28	24.769858304723293	25.17700547249741	25.13301840224575	24.920117820533545
29	24.738105315907806	25.276537152942858	25.110549651801694	24.87480787934764
30	24.703552469750576	25.12204105764701	25.30929187731396	24.865114595288457
31	25.320760965746896	24.902946712874424	24.925196942194063	24.851095379184613
32	24.88870884875311	25.41263881753152	24.97103110303126	24.727621230684107
33	24.63570683603715	25.35459079931281	25.25302225694786	24.756680107702174
34	24.957544442481563	25.239646159555434	25.579804394194667	24.22300500376834
35	24.682154072577553	25.385268389614886	25.524342038181825	24.40823549962574
36	24.374869334243893	25.311196924014478	25.616653446947822	24.697280294793803
37	25.050656749391862	25.007143520540335	24.954737942468476	24.987461787599322
38	24.841531838475703	25.48488945464979	25.07711478276059	24.596463924113916
39	25.225763564981573	24.918883200503338	25.045864090531484	24.809489143983605
40	25.50137038273891	24.956141824239275	25.014355920112713	24.528131872909103
41	24.948227319218493	25.159230634250907	25.391456796290708	24.501085250239893
42	24.989220147317408	25.325875638244348	25.322634765943807	24.362269448494438
43	24.82851185121832	25.07256331900511	25.26464269284216	24.834282136934412
44	24.374076414668917	25.53500267367949	25.33984185571142	24.751079055940174
45	24.803981587568327	25.354520724656528	25.091068184252368	24.750429503522778
46	24.813476634287735	24.996146619735786	25.181899309395213	25.008477436581266
47	25.180058269954614	25.206695511822353	25.155555169126725	24.45769104909631
48	24.815459325168238	25.492045888844405	24.75910016635042	24.933394619636935
49	25.132093308919107	25.229357616870317	24.825993323107603	24.81255575110297
50	24.750876388685793	25.750532551862054	24.781189359238343	24.717401700213813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	18.5
2	32.0
3	84.0
4	136.0
5	167.0
6	198.0
7	163.5
8	129.0
9	184.5
10	240.0
11	403.5
12	567.0
13	1177.5
14	1788.0
15	2283.0
16	2778.0
17	2925.0
18	3072.0
19	3068.0
20	3064.0
21	3829.0
22	4594.0
23	5866.5
24	7139.0
25	9703.0
26	12267.0
27	16480.0
28	20693.0
29	25858.0
30	31023.0
31	38733.0
32	46443.0
33	55478.0
34	64513.0
35	75214.5
36	85916.0
37	93834.5
38	101753.0
39	108321.5
40	114890.0
41	121203.0
42	127516.0
43	134732.0
44	141948.0
45	151912.0
46	161876.0
47	170536.5
48	179197.0
49	186870.0
50	194543.0
51	195375.5
52	196208.0
53	196060.0
54	195912.0
55	191608.5
56	187305.0
57	178705.0
58	170105.0
59	156050.5
60	141996.0
61	124838.0
62	107680.0
63	92929.0
64	78178.0
65	65947.5
66	53717.0
67	44717.5
68	35718.0
69	29168.5
70	22619.0
71	17877.5
72	13136.0
73	10864.5
74	8593.0
75	6755.0
76	4917.0
77	4082.0
78	3247.0
79	2758.5
80	2270.0
81	1765.5
82	1261.0
83	931.0
84	601.0
85	438.5
86	276.0
87	194.5
88	113.0
89	81.0
90	49.0
91	38.0
92	27.0
93	23.5
94	20.0
95	23.5
96	27.0
97	24.0
98	21.0
99	12.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031142318419140037
2	0.004623922912486528
3	2.3712425192238607E-4
4	3.9520708653731014E-5
5	3.9520708653731014E-5
6	1.5808283461492406E-4
7	0.0
8	0.0
9	0.0
10	1.5808283461492406E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.718520471134272E-4
17	0.0
18	1.9760354326865508E-4
19	0.0
20	3.161656692298481E-4
21	3.9520708653731014E-5
22	0.0
23	3.9520708653731014E-5
24	7.508934644208892E-4
25	1.5808283461492406E-4
26	4.7424850384477214E-4
27	1.5808283461492406E-4
28	0.0011856212596119305
29	3.161656692298481E-4
30	9.089762990358132E-4
31	3.161656692298481E-4
32	7.508934644208892E-4
33	3.161656692298481E-4
34	0.0010275384249970063
35	1.9760354326865508E-4
36	3.161656692298481E-4
37	0.0021341182673014747
38	0.0
39	6.718520471134272E-4
40	1.5808283461492406E-4
41	0.0010670591336507374
42	0.005374816376907418
43	0.004544881495179067
44	0.003873029448065639
45	0.002845491023068633
46	0.0028850117317223635
47	0.0010670591336507374
48	0.004663443621140259
49	0.0039520708653731015
50	0.0019365147240328195
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2530319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.38793343941144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.65861969872284	28.893331937304385
2	16.927038547689364	15.365665978577528
3	7.459512371459147	10.157155530187618
4	3.848534446088913	6.98708101113463
5	2.329468392494438	5.286487817387516
6	1.4468776298396342	3.9402471334880818
7	0.9526695108309314	3.026779024314568
8	0.6722030038626551	2.4407924157672496
9	0.4891683774127383	1.9982107579206834
>10	2.092667292152014	15.945438097360757
>50	0.08473345414029997	2.587267877650868
>100	0.03684093349530401	2.8788210717870752
>500	0.0016663418117696582	0.4927213471189667
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5808283461492406E-4
2	0.0	0.0	0.0	0.0	1.5808283461492406E-4
3	0.0	0.0	0.0	0.0	1.5808283461492406E-4
4	0.0	0.0	0.0	0.0	1.9760354326865505E-4
5	0.0	0.0	0.0	0.0	1.9760354326865505E-4
6	0.0	0.0	0.0	0.0	2.7664496057611706E-4
7	0.0	0.0	0.0	0.0	2.7664496057611706E-4
8	0.0	0.0	0.0	0.0	2.7664496057611706E-4
9	0.0	0.0	0.0	0.0	2.7664496057611706E-4
10	0.0	0.0	0.0	0.0	2.7664496057611706E-4
11	0.0	0.0	0.0	0.0	2.7664496057611706E-4
12	0.0	0.0	0.0	3.9520708653731014E-5	4.3472779519104115E-4
13	0.0	0.0	0.0	1.1856212596119304E-4	5.532899211522341E-4
14	0.0	0.0	0.0	1.1856212596119304E-4	5.532899211522341E-4
15	0.0	0.0	0.0	1.5808283461492406E-4	7.113727557671582E-4
16	0.0	0.0	0.0	1.9760354326865505E-4	7.508934644208892E-4
17	0.0	0.0	0.0	2.3712425192238607E-4	7.508934644208892E-4
18	0.0	0.0	0.0	2.3712425192238607E-4	7.508934644208892E-4
19	0.0	0.0	0.0	3.161656692298481E-4	7.508934644208892E-4
20	0.0	0.0	0.0	4.3472779519104115E-4	7.508934644208892E-4
21	0.0	0.0	0.0	4.7424850384477214E-4	7.904141730746202E-4
22	0.0	0.0	0.0	5.532899211522341E-4	7.904141730746202E-4
23	0.0	0.0	0.0	7.904141730746202E-4	8.299348817283513E-4
24	0.0	0.0	0.0	9.484970076895443E-4	8.694555903820823E-4
25	0.0	0.0	0.0	0.0012646626769193924	8.694555903820823E-4
26	0.0	0.0	0.0	0.0018179525980716266	9.484970076895443E-4
27	0.0	0.0	0.0	0.0020550768499940125	0.0010275384249970063
28	0.0	0.0	0.0	0.002489804645185054	0.0010275384249970063
29	0.0	0.0	0.0	0.003082615274991019	0.0010275384249970063
30	0.0	0.0	0.0	0.0038730294480656393	0.0010275384249970063
31	0.0	0.0	0.0	0.006283792675943231	0.0010275384249970063
32	0.0	0.0	0.0	0.008496952360552168	0.0010670591336507374
33	0.0	0.0	0.0	0.010710112045161105	0.0010670591336507374
34	0.0	0.0	0.0	0.01367416519419093	0.0011461005509581994
35	0.0	0.0	0.0	0.016282531965337178	0.0011461005509581994
36	0.0	0.0	0.0	0.019760354326865504	0.0011461005509581994
37	0.0	0.0	0.0	0.026755519758575895	0.0011461005509581994
38	0.0	0.0	0.0	0.034264454402784784	0.0011461005509581994
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2535	0.0	26.739668	1
GGTATCA	1365	0.0	22.24998	1
GTAGGAC	1490	0.0	21.556494	3
TAGGACC	1065	0.0	21.276451	4
TAGGACG	1040	0.0	19.249508	4
TGTAGGA	1740	0.0	18.713274	2
CTGTAGG	1760	0.0	18.381796	1
GTCCTAC	1465	0.0	17.876915	1
ATTTCGT	615	0.0	17.529392	42
GGACGTG	1155	0.0	17.523361	6
AGGACGT	1145	0.0	17.292133	5
TATCAAC	3910	0.0	17.161703	2
ATCAACG	3995	0.0	16.740496	3
GTCCTAA	625	0.0	16.550117	1
GATTTCG	680	0.0	16.500555	41
TCAACGC	4095	0.0	16.277971	4
TTAGGAC	800	0.0	15.949591	3
ATATGGC	2580	0.0	15.945328	15
CAACGCA	4195	0.0	15.942381	5
CGGATCG	235	3.6379788E-12	15.914801	26
>>END_MODULE