FastQCFastQC Report
Thu 2 Feb 2017
SRR4061954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366335
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66600.48743536541184995No Hit
TATCAACGCAGAGTACTTTTTTTTT39550.28946049102160154No Hit
GTATCAACGCAGAGTACATGGGCAG28550.20895314838601073No Hit
GGTATCAACGCAGAGTACTTTTTTT27260.1995118327496551No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18990.13898494878635181No Hit
ATGTAGATAAGGGAAGTCGGCAAAA16580.1213465218998269No Hit
TATCAACGCAGAGTACATGGGCAGA16460.12046825998016591No Hit
ACGCAGAGTACATGGGCAGAAATCA15250.11161245229025094No Hit
GGTATCAACGCAGAGTACATGGGGG14710.10766027365177647No Hit
GGTATCAACGCAGAGTACATGGGCA14060.1029030215869461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.016.1078381
GCCGCAT602.561133E-514.25393311
GGCGAAT400.00527285714.25236412
CTATACT500.001499579313.2997694
GTATCAA55000.012.7419141
GTGATCG853.9366187E-612.2957089
TCGGATG853.9366187E-612.29570813
TAGGGTC2400.012.2706194
CTATGCA701.089396E-412.2158659
CGTCTTA2100.012.21496915
TCTCGTC2150.011.93221312
TATGCGA658.0037693E-411.69553411
ATAGACC658.0333644E-411.690393
GCGCTAT1157.042581E-811.56840911
TGCGCTA1157.049857E-811.56756110
CGACAAG1001.9275194E-611.40063915
TCCTCGG855.310547E-511.17873610
TTCTCGT2300.011.155251511
CCTTTAA2650.011.1085351
CGAAATT600.00586938411.08476513
Produced by FastQC (version 0.11.5)