##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061954_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1366335
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230433971171053	32.0	32.0	32.0	32.0	32.0
2	30.908048172666295	32.0	32.0	32.0	32.0	32.0
3	30.93078930130605	32.0	32.0	32.0	32.0	32.0
4	30.91729041560086	32.0	32.0	32.0	32.0	32.0
5	30.899443401508414	32.0	32.0	32.0	32.0	32.0
6	34.53257290488789	36.0	36.0	36.0	32.0	36.0
7	34.44688088938657	36.0	36.0	36.0	32.0	36.0
8	34.41006634536918	36.0	36.0	36.0	32.0	36.0
9	34.484629318578534	36.0	36.0	36.0	32.0	36.0
10	34.29047195599908	36.0	36.0	36.0	32.0	36.0
11	34.51247754028112	36.0	36.0	36.0	32.0	36.0
12	34.39819663552496	36.0	36.0	36.0	32.0	36.0
13	34.41977626277597	36.0	36.0	36.0	32.0	36.0
14	34.35670168736071	36.0	36.0	36.0	32.0	36.0
15	34.32609718700026	36.0	36.0	36.0	32.0	36.0
16	34.3279847182426	36.0	36.0	36.0	32.0	36.0
17	34.27968104454617	36.0	36.0	36.0	32.0	36.0
18	34.27330266735464	36.0	36.0	36.0	32.0	36.0
19	34.247704259936256	36.0	36.0	36.0	32.0	36.0
20	34.2020836764044	36.0	36.0	36.0	32.0	36.0
21	34.18614102690775	36.0	36.0	36.0	32.0	36.0
22	34.1788390109307	36.0	36.0	36.0	32.0	36.0
23	34.152137652918206	36.0	36.0	36.0	32.0	36.0
24	34.11660903072819	36.0	36.0	36.0	32.0	36.0
25	33.80299487314604	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	29.0
5	94.0
6	295.0
7	59.0
8	234.0
9	203.0
10	93.0
11	28.0
12	60.0
13	77.0
14	316.0
15	471.0
16	692.0
17	872.0
18	1174.0
19	1731.0
20	2326.0
21	3090.0
22	4203.0
23	5639.0
24	7765.0
25	10460.0
26	14068.0
27	17762.0
28	23517.0
29	31149.0
30	40635.0
31	56559.0
32	80742.0
33	117510.0
34	275304.0
35	669175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.643752401515016	19.274477155874333	12.451503119682359	22.630267322928294
2	15.926656186051414	20.444079477023152	39.712236850194536	23.917027486730895
3	18.415497957289716	26.758406876270747	28.532512833724404	26.293582332715125
4	11.640057502730208	15.955470518139418	36.640643070247606	35.763828908882765
5	13.706209229282434	38.61948794541208	31.637125055825226	16.037177769480255
6	30.575003735026936	37.84409851096926	17.878914820879828	13.701982933123977
7	29.08105359488809	33.0992597520686	19.70870734672076	18.110979306322548
8	29.24201903561668	31.945765344626725	18.673588265033228	20.13862735472337
9	28.224311940387096	14.305938940594473	19.774509753632927	37.695239365385504
10	17.087946598380448	27.7293538143124	28.96926460640271	26.213434980904438
11	35.596295039407536	24.241076042285865	20.545790507156244	19.616838411150354
12	24.47102918262557	26.476537661549553	28.412933638806194	20.639499517018677
13	28.533937792317627	22.57992885441538	25.8170969259655	23.069036427301494
14	23.42438591169087	20.839726651747704	26.430381342208502	29.305506094352925
15	25.87430576626255	27.276478356910527	23.209063845318347	23.640152031508574
16	24.971567926275924	28.778388106599756	23.314740124983878	22.935303842140435
17	21.80838473426992	27.731736576093052	25.328832865918855	25.13104582371818
18	22.09493774303955	28.125432772521208	27.00361312389769	22.77601636054155
19	25.000586099478667	25.365213237642827	27.240658306934435	22.393542355944074
20	26.803463634765283	24.855974916485966	25.752651936939575	22.58790951180918
21	27.86893172232971	24.056411988316732	25.427957940820306	22.646698348533253
22	25.94387349519848	25.35547055017983	26.19576358671562	22.504892367906066
23	24.483045049611558	25.989191374781534	26.184473258980695	23.34329031662621
24	23.93425617703596	25.90142821979465	26.42587556847485	23.73844003469455
25	24.041924918706005	25.97100447071182	26.02748355989677	23.959587050685403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	229.0
1	229.0
2	527.5
3	826.0
4	826.0
5	826.0
6	1781.0
7	2736.0
8	2736.0
9	2736.0
10	2937.0
11	3138.0
12	3138.0
13	3138.0
14	3563.0
15	3988.0
16	3988.0
17	3988.0
18	6660.5
19	9333.0
20	9333.0
21	9333.0
22	14674.5
23	20016.0
24	20016.0
25	20016.0
26	32293.5
27	44571.0
28	44571.0
29	44571.0
30	57854.0
31	71137.0
32	71137.0
33	71137.0
34	84355.0
35	97573.0
36	97573.0
37	97573.0
38	114227.5
39	130882.0
40	130882.0
41	130882.0
42	145288.5
43	159695.0
44	159695.0
45	159695.0
46	178224.0
47	196753.0
48	196753.0
49	196753.0
50	198401.5
51	200050.0
52	200050.0
53	200050.0
54	177777.5
55	155505.0
56	155505.0
57	155505.0
58	130411.0
59	105317.0
60	105317.0
61	105317.0
62	88065.0
63	70813.0
64	70813.0
65	70813.0
66	57483.0
67	44153.0
68	44153.0
69	44153.0
70	35018.5
71	25884.0
72	25884.0
73	25884.0
74	19799.5
75	13715.0
76	13715.0
77	13715.0
78	9716.0
79	5717.0
80	5717.0
81	5717.0
82	3974.0
83	2231.0
84	2231.0
85	2231.0
86	1636.5
87	1042.0
88	1042.0
89	1042.0
90	711.0
91	380.0
92	380.0
93	380.0
94	261.0
95	142.0
96	142.0
97	142.0
98	325.5
99	509.0
100	509.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.318849330508258E-4
2	2.1956547991524775E-4
3	9.514504129660735E-4
4	0.010173200569406477
5	0.0340326493868634
6	0.06462543958838791
7	0.11139288681033567
8	0.11161245229025094
9	0.12178565285965741
10	0.1377407444001654
11	0.1465233635967753
12	0.1385458178265213
13	0.13239798438889439
14	0.11922405559397951
15	0.13766755590686033
16	0.12288348025923364
17	0.12705522437762334
18	0.11622332736847113
19	0.10092693226770888
20	0.09477909883008194
21	0.09558417225643785
22	0.10641606926559007
23	0.08262980894143823
24	0.09360808293720062
25	0.0898754697786414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1366335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.347170756858542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.92764125925042	17.587067210909833
2	14.187545481550664	8.327286397355284
3	7.279138239700014	6.408663386497651
4	4.386948976975172	5.149781629156651
5	2.9208500364911574	4.285934238804125
6	2.1275062769243798	3.7461773997112897
7	1.5481764438994479	3.180421892260419
8	1.1750539756736667	2.7587607738096476
9	0.9257441096227624	2.4451173416029666
>10	5.058798851356225	26.079674183080364
>50	0.27458944251000916	5.494928841410229
>100	0.17000624677061285	9.184754225040436
>500	0.013250485300049306	2.6993048926126018
>1k	0.004500164818884669	2.1634792978653925
>5k	2.500091566047039E-4	0.4886482898831185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6660	0.48743536541184995	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3955	0.28946049102160154	No Hit
GTATCAACGCAGAGTACATGGGCAG	2855	0.20895314838601073	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2726	0.1995118327496551	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1899	0.13898494878635181	No Hit
ATGTAGATAAGGGAAGTCGGCAAAA	1658	0.1213465218998269	No Hit
TATCAACGCAGAGTACATGGGCAGA	1646	0.12046825998016591	No Hit
ACGCAGAGTACATGGGCAGAAATCA	1525	0.11161245229025094	No Hit
GGTATCAACGCAGAGTACATGGGGG	1471	0.10766027365177647	No Hit
GGTATCAACGCAGAGTACATGGGCA	1406	0.1029030215869461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.318849330508258E-5	0.0
6	0.0	0.0	0.0	7.318849330508258E-5	0.0
7	7.318849330508258E-5	0.0	0.0	7.318849330508258E-5	0.0
8	7.318849330508258E-5	0.0	0.0	7.318849330508258E-5	0.0
9	7.318849330508258E-5	0.0	0.0	1.4637698661016516E-4	0.0
10	7.318849330508258E-5	0.0	0.0	2.1956547991524772E-4	0.0
11	7.318849330508258E-5	0.0	0.0	2.1956547991524772E-4	0.0
12	7.318849330508258E-5	0.0	0.0	2.927539732203303E-4	0.0
13	7.318849330508258E-5	0.0	0.0	2.927539732203303E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1745	0.0	16.107838	1
GCCGCAT	60	2.561133E-5	14.253933	11
GGCGAAT	40	0.005272857	14.252364	12
CTATACT	50	0.0014995793	13.299769	4
GTATCAA	5500	0.0	12.741914	1
GTGATCG	85	3.9366187E-6	12.295708	9
TCGGATG	85	3.9366187E-6	12.295708	13
TAGGGTC	240	0.0	12.270619	4
CTATGCA	70	1.089396E-4	12.215865	9
CGTCTTA	210	0.0	12.214969	15
TCTCGTC	215	0.0	11.932213	12
TATGCGA	65	8.0037693E-4	11.695534	11
ATAGACC	65	8.0333644E-4	11.69039	3
GCGCTAT	115	7.042581E-8	11.568409	11
TGCGCTA	115	7.049857E-8	11.567561	10
CGACAAG	100	1.9275194E-6	11.400639	15
TCCTCGG	85	5.310547E-5	11.178736	10
TTCTCGT	230	0.0	11.1552515	11
CCTTTAA	265	0.0	11.108535	1
CGAAATT	60	0.005869384	11.084765	13
>>END_MODULE