FastQCFastQC Report
Thu 2 Feb 2017
SRR4061954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366335
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.13803349837338574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.032.2695121
GTATCAA59400.027.903231
TCGCGTC952.6859198E-823.15793224
TTTCGCG1401.8553692E-1020.42860622
GTTTCGC1101.2663986E-719.99930221
TTCGCGT1152.0218431E-719.13046623
TATCAAC91250.018.2262132
ATCAACG93850.017.6275143
TCAACGC95650.017.2727894
TGCGCTA1802.8558134E-1017.11051410
AACGCAG98200.016.846666
CGTCGTA1053.7760532E-516.7613210
CGCGTCT1602.1296728E-816.50002925
TGTACCG1602.1307642E-816.4994245
CAACGCA100250.016.4802235
GCGCTAT1753.6270649E-916.34228711
TAGGACC5000.016.2794324
GTATTAG1906.1299943E-1016.2188631
GTATAGT1958.8584784E-1015.8029961
CAGAGCG2101.5097612E-1015.713737514
Produced by FastQC (version 0.11.5)