##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1366335
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254467608602575	32.0	32.0	32.0	32.0	32.0
2	31.50992106620997	32.0	32.0	32.0	32.0	32.0
3	31.61123882503193	32.0	32.0	32.0	32.0	32.0
4	31.68251270735215	32.0	32.0	32.0	32.0	32.0
5	31.664706678815957	32.0	32.0	32.0	32.0	32.0
6	35.310109160637765	36.0	36.0	36.0	36.0	36.0
7	35.307679302660034	36.0	36.0	36.0	36.0	36.0
8	35.26993746043247	36.0	36.0	36.0	36.0	36.0
9	35.33801593313499	36.0	36.0	36.0	36.0	36.0
10	35.24557374289614	36.0	36.0	36.0	36.0	36.0
11	35.35168022483505	36.0	36.0	36.0	36.0	36.0
12	35.2936036916276	36.0	36.0	36.0	36.0	36.0
13	35.3055195102226	36.0	36.0	36.0	36.0	36.0
14	35.27671471491252	36.0	36.0	36.0	36.0	36.0
15	35.25264667888914	36.0	36.0	36.0	36.0	36.0
16	35.26515825181965	36.0	36.0	36.0	36.0	36.0
17	35.248603746518974	36.0	36.0	36.0	36.0	36.0
18	35.243527392623335	36.0	36.0	36.0	36.0	36.0
19	35.21400681384873	36.0	36.0	36.0	36.0	36.0
20	35.20115345065449	36.0	36.0	36.0	36.0	36.0
21	35.186103700776165	36.0	36.0	36.0	36.0	36.0
22	35.1857545916631	36.0	36.0	36.0	36.0	36.0
23	35.151290862050665	36.0	36.0	36.0	36.0	36.0
24	35.12559804147592	36.0	36.0	36.0	36.0	36.0
25	35.109926921289436	36.0	36.0	36.0	36.0	36.0
26	35.06493209937533	36.0	36.0	36.0	36.0	36.0
27	35.04709313601715	36.0	36.0	36.0	36.0	36.0
28	35.025504726146956	36.0	36.0	36.0	36.0	36.0
29	34.98004735295517	36.0	36.0	36.0	36.0	36.0
30	34.96915690515137	36.0	36.0	36.0	36.0	36.0
31	34.946148638511055	36.0	36.0	36.0	36.0	36.0
32	34.93725330903475	36.0	36.0	36.0	36.0	36.0
33	34.901780310099646	36.0	36.0	36.0	36.0	36.0
34	34.86396088806918	36.0	36.0	36.0	32.0	36.0
35	34.84133393347898	36.0	36.0	36.0	32.0	36.0
36	34.81205048542268	36.0	36.0	36.0	32.0	36.0
37	34.79763454789638	36.0	36.0	36.0	32.0	36.0
38	34.78180021736983	36.0	36.0	36.0	32.0	36.0
39	34.7189210552317	36.0	36.0	36.0	32.0	36.0
40	34.72618135376756	36.0	36.0	36.0	32.0	36.0
41	34.662590067589576	36.0	36.0	36.0	32.0	36.0
42	34.66551394789711	36.0	36.0	36.0	32.0	36.0
43	34.61794069536388	36.0	36.0	36.0	32.0	36.0
44	34.52907522679284	36.0	36.0	36.0	32.0	36.0
45	34.50325798577948	36.0	36.0	36.0	32.0	36.0
46	34.45155031525943	36.0	36.0	36.0	32.0	36.0
47	34.42632077784731	36.0	36.0	36.0	32.0	36.0
48	34.39037498124545	36.0	36.0	36.0	32.0	36.0
49	34.30809281764721	36.0	36.0	36.0	32.0	36.0
50	33.945842710609035	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	15.0
21	46.0
22	139.0
23	379.0
24	874.0
25	2050.0
26	4163.0
27	7435.0
28	12279.0
29	18935.0
30	27464.0
31	39626.0
32	58830.0
33	94448.0
34	203933.0
35	895714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25355181335391	19.891545783727036	12.891658528085792	22.963243874833264
2	15.484731332643372	20.829753004914135	39.344283892840465	24.34123176960202
3	17.956342744222642	26.917021938411434	28.832159259327028	26.294476058038903
4	11.396627764514387	16.26725237021109	36.69805479480127	35.63806507047325
5	13.362106190726426	38.89451627493717	31.575515210775695	16.167862323560712
6	30.824741588970756	37.92719333748557	17.643528544693783	13.604536528849891
7	29.085839124372864	33.023672818159525	19.768065664716193	18.12242239275141
8	29.29442633029235	32.04594773609693	18.664383185675547	19.99524274793517
9	28.330387496477805	14.064998700904244	19.72437213421306	37.8802416684049
10	17.0095306125735	27.824675372200353	29.106572770640266	26.059221244585878
11	35.80798266896478	24.00304464132149	20.66740587044905	19.521566819264674
12	24.578672141165967	26.353200349841	28.37488610040729	20.69324140858574
13	28.667127754174487	22.414488394134676	25.988136145235245	22.930247706455592
14	23.594799225665742	20.572187640659134	26.46730121090362	29.365711922771503
15	26.000797754577025	27.213677465628855	23.118561699729568	23.66696308006455
16	25.10621511185796	28.509124070228957	23.43802490026209	22.946635917650994
17	21.912708084035028	27.57398441816978	25.489429751854413	25.02387774594078
18	22.246041045630896	27.955264199869283	27.080660424654894	22.718034329844922
19	25.125316997661628	25.217607687719333	27.36166459909173	22.295410715527307
20	26.914001490127536	24.745119393175568	25.801089930221632	22.53978918647526
21	28.089178780590984	23.83348434570171	25.32997056356645	22.74736631014086
22	25.99457673264609	25.27096209933874	26.284329977640912	22.45013119037425
23	24.465778108264967	25.836015085634322	26.336112792415907	23.362094013684803
24	24.118434920077288	25.91559810293343	26.37142104338662	23.59454593360267
25	24.29138745195165	25.6893639320458	26.135009646264596	23.884238969737957
26	23.950035569685845	26.022518685063368	26.677483949728177	23.34996179552261
27	23.700493147316234	26.40555767803584	26.470110354337105	23.42383882031082
28	23.036764555198065	26.546128421243694	26.723027469487292	23.694079554070946
29	23.66756200917787	25.718018341103537	26.478230003000743	24.136189646717852
30	23.76092259567495	26.265828111883096	26.807209736525657	23.1660395559163
31	24.61989305626915	26.043565005716058	25.641903908730416	23.694638029284373
32	23.1984846910172	25.877940924613668	26.53122763155391	24.39234675281522
33	23.428036942677956	25.953921920937283	27.19673754494695	23.421303591437812
34	23.394153638971837	25.48326892675215	27.646012647110485	23.476564787165525
35	23.98886067055543	25.85305160539548	26.303967562741065	23.85412016130803
36	24.220979966801625	25.864577569726833	26.093438490791403	23.82100397268014
37	24.03332769772036	25.329665061614815	26.002132750157177	24.634874490507652
38	23.417902637347357	26.257689366077862	26.716434842114122	23.607973154460655
39	24.076520481553832	24.99467550593672	27.00898101259801	23.919822999911442
40	24.020756256701322	25.53934430428848	26.711677590049295	23.728221848960906
41	24.460741261197963	26.489621757714154	26.035116224603318	23.014520756484572
42	23.669936154593277	26.668467190575207	26.25771352493543	23.403883129896087
43	23.652872987781404	26.285426538376523	26.30570059680387	23.7559998770382
44	23.663004145587408	25.71654838860783	26.591044828555365	24.02940263724939
45	24.407345331586537	25.738887953685474	26.066485643814364	23.78728107091363
46	24.778800571762737	26.299841689349563	25.117671728264856	23.803686010622844
47	23.7472919960185	26.005547748697232	26.408235259675628	23.838924995608643
48	23.542000756803365	26.659679288107135	25.918026631573632	23.880293323515872
49	24.116728293678328	25.82047363505684	26.08842671069851	23.974371360566323
50	23.831194970394712	26.522311920428017	25.75111065570771	23.895382453469562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	63.0
2	77.0
3	185.5
4	294.0
5	346.5
6	399.0
7	383.0
8	367.0
9	532.0
10	697.0
11	1015.0
12	1333.0
13	2473.5
14	3614.0
15	4809.0
16	6004.0
17	5851.5
18	5699.0
19	5469.0
20	5239.0
21	5320.0
22	5401.0
23	5520.0
24	5639.0
25	6732.5
26	7826.0
27	10252.5
28	12679.0
29	15300.0
30	17921.0
31	20524.0
32	23127.0
33	29083.5
34	35040.0
35	41030.0
36	47020.0
37	55937.0
38	64854.0
39	73326.0
40	81798.0
41	87142.0
42	92486.0
43	94843.0
44	97200.0
45	98826.5
46	100453.0
47	103196.5
48	105940.0
49	106812.5
50	107685.0
51	105193.0
52	102701.0
53	97685.5
54	92670.0
55	87311.5
56	81953.0
57	75049.5
58	68146.0
59	60439.5
60	52733.0
61	45492.0
62	38251.0
63	33611.0
64	28971.0
65	25424.5
66	21878.0
67	18983.0
68	16088.0
69	14014.5
70	11941.0
71	9894.5
72	7848.0
73	6922.5
74	5997.0
75	4643.0
76	3289.0
77	2740.5
78	2192.0
79	1753.0
80	1314.0
81	978.0
82	642.0
83	514.5
84	387.0
85	299.0
86	211.0
87	161.0
88	111.0
89	89.5
90	68.0
91	57.0
92	46.0
93	33.5
94	21.0
95	19.0
96	17.0
97	15.5
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030226847734999104
2	0.005342760011271028
3	7.318849330508258E-4
4	7.318849330508256E-5
5	7.318849330508256E-5
6	2.9275397322033026E-4
7	0.0
8	0.0
9	0.0
10	7.318849330508256E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.9275397322033026E-4
17	0.0
18	1.4637698661016513E-4
19	0.0
20	6.586964397457431E-4
21	7.318849330508256E-5
22	0.0
23	1.4637698661016513E-4
24	0.0010978273995762386
25	2.1956547991524775E-4
26	2.1956547991524775E-4
27	6.586964397457431E-4
28	0.001390581372796569
29	3.659424665254129E-4
30	0.001171015892881321
31	6.586964397457431E-4
32	9.514504129660735E-4
33	1.4637698661016513E-4
34	0.0010978273995762386
35	3.659424665254129E-4
36	2.1956547991524775E-4
37	0.001756523839321982
38	0.0
39	5.855079464406605E-4
40	0.0
41	0.0010978273995762386
42	0.00483044055813545
43	0.004171744118389707
44	0.0046108750782202015
45	0.0018297123326270644
46	0.0024884087723728078
47	0.0010978273995762386
48	0.00453768658491512
49	0.0037326131585592114
50	0.0018297123326270644
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1366335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.695039504614954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.05822719738217	19.636061107955506
2	14.314742445724034	9.360421395226718
3	7.338812576730288	7.198283013394856
4	4.282699418994394	5.600921067616529
5	2.9193435838573722	4.772402690088049
6	2.1223721055656988	4.163462390097801
7	1.5908206551136677	3.640836091458916
8	1.192075291599302	3.117995900105166
9	0.9259383202563581	2.7246230963656597
>10	4.929962762460079	27.528613910122434
>50	0.21312546093087947	4.69286357589872
>100	0.10737796406654132	6.489028098045815
>500	0.004277106453280624	0.9356779003800954
>1k	2.251108659621381E-4	0.13880976324367653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1886	0.13803349837338574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.318849330508258E-5	0.0
6	0.0	0.0	0.0	7.318849330508258E-5	0.0
7	0.0	0.0	0.0	7.318849330508258E-5	0.0
8	0.0	0.0	0.0	7.318849330508258E-5	0.0
9	0.0	0.0	0.0	7.318849330508258E-5	0.0
10	0.0	0.0	0.0	1.4637698661016516E-4	0.0
11	0.0	0.0	0.0	1.4637698661016516E-4	0.0
12	0.0	0.0	0.0	2.1956547991524772E-4	0.0
13	0.0	0.0	0.0	2.1956547991524772E-4	0.0
14	0.0	0.0	0.0	2.1956547991524772E-4	0.0
15	0.0	0.0	0.0	2.1956547991524772E-4	0.0
16	0.0	0.0	0.0	2.927539732203303E-4	0.0
17	0.0	0.0	0.0	3.659424665254129E-4	0.0
18	7.318849330508258E-5	0.0	0.0	4.3913095983049544E-4	0.0
19	7.318849330508258E-5	0.0	0.0	4.3913095983049544E-4	0.0
20	2.927539732203303E-4	0.0	0.0	5.12319453135578E-4	0.0
21	2.927539732203303E-4	0.0	0.0	5.855079464406606E-4	0.0
22	2.927539732203303E-4	0.0	0.0	0.0010978273995762386	0.0
23	2.927539732203303E-4	0.0	0.0	0.0014637698661016515	0.0
24	2.927539732203303E-4	0.0	0.0	0.0017565238393219818	0.0
25	2.927539732203303E-4	0.0	0.0	0.002049277812542312	0.0
26	2.927539732203303E-4	0.0	0.0	0.0026347857589829726	0.0
27	2.927539732203303E-4	0.0	0.0	0.002781162745593138	0.0
28	3.659424665254129E-4	0.0	0.0	0.004098555625084624	0.0
29	3.659424665254129E-4	0.0	0.0	0.005342760011271028	0.0
30	5.855079464406606E-4	0.0	0.0	0.008343488236779413	0.0
31	5.855079464406606E-4	0.0	0.0	0.012734797835084369	0.0
32	6.586964397457431E-4	0.0	0.0	0.018516688806185892	0.0
33	6.586964397457431E-4	0.0	0.0	0.02415220279067725	0.0
34	6.586964397457431E-4	0.0	0.0	0.031763806094405836	0.0
35	6.586964397457431E-4	0.0	0.0	0.04025367131779541	0.0
36	7.318849330508258E-4	0.0	0.0	0.05145151079347305	0.0
37	8.050734263559084E-4	0.0	7.318849330508258E-5	0.07033414206618435	0.0
38	8.050734263559084E-4	0.0	7.318849330508258E-5	0.0932421404706752	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2135	0.0	32.269512	1
GTATCAA	5940	0.0	27.90323	1
TCGCGTC	95	2.6859198E-8	23.157932	24
TTTCGCG	140	1.8553692E-10	20.428606	22
GTTTCGC	110	1.2663986E-7	19.999302	21
TTCGCGT	115	2.0218431E-7	19.130466	23
TATCAAC	9125	0.0	18.226213	2
ATCAACG	9385	0.0	17.627514	3
TCAACGC	9565	0.0	17.272789	4
TGCGCTA	180	2.8558134E-10	17.110514	10
AACGCAG	9820	0.0	16.84666	6
CGTCGTA	105	3.7760532E-5	16.76132	10
CGCGTCT	160	2.1296728E-8	16.500029	25
TGTACCG	160	2.1307642E-8	16.499424	5
CAACGCA	10025	0.0	16.480223	5
GCGCTAT	175	3.6270649E-9	16.342287	11
TAGGACC	500	0.0	16.279432	4
GTATTAG	190	6.1299943E-10	16.218863	1
GTATAGT	195	8.8584784E-10	15.802996	1
CAGAGCG	210	1.5097612E-10	15.7137375	14
>>END_MODULE