FastQCFastQC Report
Thu 2 Feb 2017
SRR4061952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185003
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17680.149197934519997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG350.002177632616.2769181
GGTATCA5650.015.2926221
CGATTTA456.760187E-414.77790918
GCGTTAT551.968531E-413.8107191
GTCCTAA1257.2759576E-1213.6726111
GACGCTC707.248873E-613.573278
CGACCAT1850.013.35372710
ATCGCCA1650.012.6667816
GTATAGG1001.4514626E-712.3433281
TCGATTT853.944404E-612.29318717
AACCGCG701.0895254E-412.2154277
CTAAGGT701.091246E-412.2133634
CGGTTTC550.003064585612.0925513
TAGGACT1103.814057E-812.0899954
CGCATCG1701.8189894E-1211.73688513
ACTCTAA1950.011.69438810
AATCGAT658.0203416E-411.69241115
GTAGGGA658.040266E-411.6889543
CGTTATT658.048818E-411.6874732
GTATCAA14100.011.6497331
Produced by FastQC (version 0.11.5)