##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061952_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185003
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2179926970649	32.0	32.0	32.0	32.0	32.0
2	30.720617584934384	32.0	32.0	32.0	32.0	32.0
3	30.744522165766668	32.0	32.0	32.0	32.0	32.0
4	30.753385434467255	32.0	32.0	32.0	32.0	32.0
5	30.653589906523443	32.0	32.0	32.0	32.0	32.0
6	34.31263211991868	36.0	36.0	36.0	32.0	36.0
7	34.25473437619989	36.0	36.0	36.0	32.0	36.0
8	34.2004535009616	36.0	36.0	36.0	32.0	36.0
9	34.34524216394389	36.0	36.0	36.0	32.0	36.0
10	34.00010295332586	36.0	36.0	36.0	32.0	36.0
11	34.330832917722574	36.0	36.0	36.0	32.0	36.0
12	34.1363903720075	36.0	36.0	36.0	32.0	36.0
13	34.237550453458766	36.0	36.0	36.0	32.0	36.0
14	34.12472795427522	36.0	36.0	36.0	32.0	36.0
15	34.0715854727794	36.0	36.0	36.0	32.0	36.0
16	34.08418122148214	36.0	36.0	36.0	32.0	36.0
17	34.01559405334839	36.0	36.0	36.0	32.0	36.0
18	34.01317886958936	36.0	36.0	36.0	32.0	36.0
19	34.01768687505432	36.0	36.0	36.0	32.0	36.0
20	34.004820240961415	36.0	36.0	36.0	32.0	36.0
21	33.98938061760181	36.0	36.0	36.0	32.0	36.0
22	33.94486427460521	36.0	36.0	36.0	32.0	36.0
23	33.920449990421965	36.0	36.0	36.0	32.0	36.0
24	33.89404583785864	36.0	36.0	36.0	32.0	36.0
25	33.53100118733877	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	109.0
6	275.0
7	67.0
8	221.0
9	172.0
10	95.0
11	27.0
12	44.0
13	55.0
14	171.0
15	290.0
16	430.0
17	547.0
18	813.0
19	1163.0
20	1848.0
21	2581.0
22	3804.0
23	5637.0
24	8000.0
25	11201.0
26	15063.0
27	19193.0
28	25293.0
29	32800.0
30	42546.0
31	57119.0
32	80031.0
33	111309.0
34	241610.0
35	522472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.364111548244125	17.29873737757322	11.80908932872234	25.528061745460313
2	17.01146243758444	19.049519789434775	37.45726796855024	26.481749804430542
3	19.58349296792062	22.62619852522958	27.726375909495204	30.063932597354594
4	13.135904704916248	15.381388797223655	34.64777059366156	36.83493590419853
5	15.252148264209273	36.213331352290005	32.9431747288658	15.591345654634925
6	35.32630128264132	34.29606729285752	16.315613027224355	14.062018397276804
7	31.27584706563201	29.723861461840333	19.6018231817817	19.398468290745956
8	28.49501475591694	33.09414638308946	18.46614503465296	19.944693826340643
9	26.949156695527414	14.66017292790277	18.285186552755853	40.10548382381396
10	16.98969110497597	26.435779192409324	30.226467806991526	26.348061895623175
11	37.45177465060127	21.266565645211863	21.530753933878557	19.750905770308314
12	24.79477615093523	23.333998117196888	28.175286603534733	23.695939128333144
13	29.61476352518964	19.407082794280562	24.74397986146731	26.234173819062484
14	23.615305193038548	19.524124960077597	24.560262127538767	32.30030771934509
15	25.46413628692661	27.00056704528841	21.61042552058729	25.92487114719769
16	26.007585993035892	25.759338637951767	23.221436133762907	25.011639235249433
17	24.21977323695876	26.181008215048017	24.56275698431181	25.03646156368141
18	24.995712201258712	25.10411113298428	25.51734509640577	24.38283156935124
19	26.075181160950674	24.878479819286778	24.61296857286227	24.433370446900284
20	26.165486446830133	24.328465753632948	24.406180336689985	25.099867462846937
21	26.753175227984578	24.11289586874035	24.155553227937276	24.978375675337798
22	26.338443550642175	24.23859958587725	24.56469379669124	24.858263066789334
23	24.772585606632646	24.374008625276826	25.05418248220804	25.79922328588249
24	24.988680864579216	24.855047489686072	25.025425819116837	25.130845826617875
25	25.047682761393602	24.52904298290516	24.79039012861253	25.63288412708871
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	324.0
1	324.0
2	346.0
3	368.0
4	368.0
5	368.0
6	733.0
7	1098.0
8	1098.0
9	1098.0
10	1313.5
11	1529.0
12	1529.0
13	1529.0
14	2052.5
15	2576.0
16	2576.0
17	2576.0
18	4333.0
19	6090.0
20	6090.0
21	6090.0
22	10021.5
23	13953.0
24	13953.0
25	13953.0
26	21088.5
27	28224.0
28	28224.0
29	28224.0
30	36601.0
31	44978.0
32	44978.0
33	44978.0
34	56280.5
35	67583.0
36	67583.0
37	67583.0
38	80212.5
39	92842.0
40	92842.0
41	92842.0
42	107290.0
43	121738.0
44	121738.0
45	121738.0
46	137296.5
47	152855.0
48	152855.0
49	152855.0
50	158854.0
51	164853.0
52	164853.0
53	164853.0
54	156193.0
55	147533.0
56	147533.0
57	147533.0
58	135943.5
59	124354.0
60	124354.0
61	124354.0
62	109566.0
63	94778.0
64	94778.0
65	94778.0
66	76835.0
67	58892.0
68	58892.0
69	58892.0
70	44667.0
71	30442.0
72	30442.0
73	30442.0
74	23030.5
75	15619.0
76	15619.0
77	15619.0
78	12088.0
79	8557.0
80	8557.0
81	8557.0
82	5865.5
83	3174.0
84	3174.0
85	3174.0
86	2313.5
87	1453.0
88	1453.0
89	1453.0
90	984.5
91	516.0
92	516.0
93	516.0
94	330.0
95	144.0
96	144.0
97	144.0
98	337.0
99	530.0
100	530.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.594917481221566E-4
2	1.6877594402714592E-4
3	4.219398600678648E-4
4	0.011476764193845922
5	0.03729948362999925
6	0.0680167054429398
7	0.11459886599443209
8	0.11898704053913786
9	0.12691950990841372
10	0.1397464816544768
11	0.1481852788558341
12	0.14016842151454467
13	0.13502075522171675
14	0.12295327522377579
15	0.14143424109474828
16	0.12708828585244086
17	0.13206717620124167
18	0.11915581648316502
19	0.10540057704495262
20	0.0999153588640704
21	0.09713055578762247
22	0.10936681172959055
23	0.08784787886612945
24	0.09848076333983966
25	0.09594912417943247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1185003.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.831253309890485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5323604159048	46.20157188651712
2	13.338411835018421	15.694309708352197
3	3.7675211546354683	6.649439741961909
4	1.5362314961551022	3.6151369717173143
5	0.771914039866563	2.270633520642533
6	0.47023657615617764	1.6598764276451814
7	0.2868307610970049	1.18122292142166
8	0.21317820767482765	1.003323290869298
9	0.15094729593816103	0.7992376743402859
>10	0.8005679643893864	8.465036539274692
>50	0.06693858986858335	2.752076623119371
>100	0.058663777369554	6.922535696873753
>500	0.005044790870054079	1.997405917216695
>1k	0.0011530950560123608	0.788193080047938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1768	0.149197934519997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.438797201357296E-5	0.0	0.0	0.0
6	0.0	8.438797201357296E-5	0.0	0.0	8.438797201357296E-5
7	0.0	8.438797201357296E-5	0.0	0.0	8.438797201357296E-5
8	0.0	8.438797201357296E-5	0.0	0.0	8.438797201357296E-5
9	0.0	8.438797201357296E-5	0.0	0.0	8.438797201357296E-5
10	0.0	8.438797201357296E-5	0.0	0.0	8.438797201357296E-5
11	0.0	8.438797201357296E-5	0.0	8.438797201357296E-5	8.438797201357296E-5
12	0.0	8.438797201357296E-5	0.0	8.438797201357296E-5	3.3755188805429184E-4
13	0.0	8.438797201357296E-5	0.0	8.438797201357296E-5	3.3755188805429184E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTAG	35	0.0021776326	16.276918	1
GGTATCA	565	0.0	15.292622	1
CGATTTA	45	6.760187E-4	14.777909	18
GCGTTAT	55	1.968531E-4	13.810719	1
GTCCTAA	125	7.2759576E-12	13.672611	1
GACGCTC	70	7.248873E-6	13.57327	8
CGACCAT	185	0.0	13.353727	10
ATCGCCA	165	0.0	12.66678	16
GTATAGG	100	1.4514626E-7	12.343328	1
TCGATTT	85	3.944404E-6	12.293187	17
AACCGCG	70	1.0895254E-4	12.215427	7
CTAAGGT	70	1.091246E-4	12.213363	4
CGGTTTC	55	0.0030645856	12.09255	13
TAGGACT	110	3.814057E-8	12.089995	4
CGCATCG	170	1.8189894E-12	11.736885	13
ACTCTAA	195	0.0	11.694388	10
AATCGAT	65	8.0203416E-4	11.692411	15
GTAGGGA	65	8.040266E-4	11.688954	3
CGTTATT	65	8.048818E-4	11.687473	2
GTATCAA	1410	0.0	11.649733	1
>>END_MODULE