FastQCFastQC Report
Thu 2 Feb 2017
SRR4061952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185003
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12180.10278454991253186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.028.6318661
GTATCAA15200.026.1989521
GTCCTAA2050.018.2450051
GCTAGAC851.4310655E-418.1172123
TCTATTC1254.8535003E-717.5995773
TATACTG1651.6589183E-917.3329185
GTCCTAT3050.017.3125231
CTTACAC3200.017.1870883
GTCCTAC3600.017.1121461
ATCAACG23500.016.9442753
CGCAATA2600.016.92409936
AGTGATC1451.2495002E-716.6892578
TAGGACG1854.2200554E-1016.6482494
TCAACGC23850.016.6033764
CTATTCC3200.016.4996054
CAACGCA24350.016.2624445
GTGTATA3250.016.2471351
CGGATCG953.3457793E-416.21082126
CCCGTAC953.346855E-416.2101383
AACGCAG24650.016.0645246
Produced by FastQC (version 0.11.5)