##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185003
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285730078320476	32.0	32.0	32.0	32.0	32.0
2	31.447013214312538	32.0	32.0	32.0	32.0	32.0
3	31.571335262442375	32.0	32.0	32.0	32.0	32.0
4	31.65202704128175	32.0	32.0	32.0	32.0	32.0
5	31.625495462880686	32.0	32.0	32.0	32.0	32.0
6	35.268872737031046	36.0	36.0	36.0	36.0	36.0
7	35.27934781599709	36.0	36.0	36.0	36.0	36.0
8	35.22805427496808	36.0	36.0	36.0	36.0	36.0
9	35.30834014766207	36.0	36.0	36.0	36.0	36.0
10	35.1954914882072	36.0	36.0	36.0	36.0	36.0
11	35.310613559628116	36.0	36.0	36.0	36.0	36.0
12	35.23662218576662	36.0	36.0	36.0	36.0	36.0
13	35.26642717360209	36.0	36.0	36.0	36.0	36.0
14	35.22947030513847	36.0	36.0	36.0	36.0	36.0
15	35.207117619111514	36.0	36.0	36.0	36.0	36.0
16	35.219549655148555	36.0	36.0	36.0	36.0	36.0
17	35.19929147858697	36.0	36.0	36.0	36.0	36.0
18	35.19331174689009	36.0	36.0	36.0	36.0	36.0
19	35.19489486524507	36.0	36.0	36.0	36.0	36.0
20	35.1932282027978	36.0	36.0	36.0	36.0	36.0
21	35.18039448001397	36.0	36.0	36.0	36.0	36.0
22	35.16146288237245	36.0	36.0	36.0	36.0	36.0
23	35.12764187094885	36.0	36.0	36.0	36.0	36.0
24	35.11359464912747	36.0	36.0	36.0	36.0	36.0
25	35.10048919707376	36.0	36.0	36.0	36.0	36.0
26	35.059346685198264	36.0	36.0	36.0	36.0	36.0
27	35.039659815207216	36.0	36.0	36.0	36.0	36.0
28	35.02784212360644	36.0	36.0	36.0	36.0	36.0
29	34.99814599625486	36.0	36.0	36.0	36.0	36.0
30	34.98285320796656	36.0	36.0	36.0	36.0	36.0
31	34.981554477077275	36.0	36.0	36.0	36.0	36.0
32	34.95871149693292	36.0	36.0	36.0	36.0	36.0
33	34.93574615422914	36.0	36.0	36.0	36.0	36.0
34	34.93490480614817	36.0	36.0	36.0	36.0	36.0
35	34.91501118562569	36.0	36.0	36.0	36.0	36.0
36	34.895341193229044	36.0	36.0	36.0	32.0	36.0
37	34.87569229782541	36.0	36.0	36.0	32.0	36.0
38	34.86327882714221	36.0	36.0	36.0	32.0	36.0
39	34.83782910254236	36.0	36.0	36.0	32.0	36.0
40	34.836869611300564	36.0	36.0	36.0	32.0	36.0
41	34.80519542988499	36.0	36.0	36.0	32.0	36.0
42	34.77170690707112	36.0	36.0	36.0	32.0	36.0
43	34.77793220776656	36.0	36.0	36.0	32.0	36.0
44	34.731294351153544	36.0	36.0	36.0	32.0	36.0
45	34.71937539398635	36.0	36.0	36.0	32.0	36.0
46	34.69747249585022	36.0	36.0	36.0	32.0	36.0
47	34.670406741586305	36.0	36.0	36.0	32.0	36.0
48	34.6465848609666	36.0	36.0	36.0	32.0	36.0
49	34.62898068612485	36.0	36.0	36.0	32.0	36.0
50	34.23000110548244	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	26.0
22	81.0
23	214.0
24	573.0
25	1301.0
26	2823.0
27	5283.0
28	9114.0
29	14612.0
30	21941.0
31	32056.0
32	49322.0
33	83562.0
34	191552.0
35	772536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15789966834114	17.743960035487245	12.175400181150398	25.922740115021213
2	16.507292718927143	19.512063388382117	37.038134130667224	26.942509762023516
3	18.95647169322506	23.017656554984434	28.09774523016475	29.92812652162576
4	12.902920920875305	15.783673121502645	34.79147310175586	36.52193285586619
5	14.921422007238814	36.43693127685124	32.868157917166315	15.773488798743628
6	35.43268812885074	34.50610506844309	16.180604371818035	13.880602430888128
7	31.318232949621223	29.73241417954216	19.66332574685465	19.286027123981963
8	28.432333082701057	33.26945163851906	18.47413044523938	19.824084833540507
9	27.19368642948583	14.398528948871858	18.29328702121429	40.11449760042802
10	16.857777455227925	26.44147436038083	30.389147022536672	26.311601161854583
11	37.65602281175659	21.220958934281178	21.43564193508371	19.68737631887852
12	24.775717867380926	23.33158650231265	28.189717663162035	23.70297796714439
13	29.78456594624655	19.318854045095243	24.74913565619665	26.14744435246156
14	23.683062405749183	19.41910695584737	24.550655146020727	32.34717549238272
15	25.528542965714013	26.947273551206198	21.57032513841737	25.953858344662418
16	26.110405529137253	25.701036713235077	23.251490512618197	24.937067245009473
17	24.428798914433127	26.015377176260312	24.531245912457607	25.02457799684895
18	25.113924050632914	25.05552742616034	25.518227848101265	24.312320675105486
19	26.112360991795796	24.7427430502227	24.706793743808028	24.43810221417348
20	26.30365088915687	24.143817842884253	24.489556531271333	25.062974736687543
21	26.90335282417483	24.014325726307405	24.27297529706726	24.809346152450505
22	26.33554514207981	24.180445112797184	24.607026311325793	24.876983433797214
23	24.875991770478024	24.19945282792772	25.195738066264866	25.72881733532939
24	25.146583268072696	24.757973049607084	24.936202100942452	25.159241581377767
25	25.164409421442553	24.296813752585443	25.00651899284303	25.532257833128973
26	24.95430372034383	25.126793462942555	25.184515079350344	24.73438773736327
27	25.518208062974	24.674070904820855	24.66394429690539	25.14377673529975
28	25.253990556842492	24.436511854580438	25.152892230703344	25.15660535787373
29	24.939366781603958	24.692952031824632	25.112447826740052	25.255233359831358
30	25.034662769021487	24.790272314764103	25.142427482449204	25.03263743376521
31	25.601185493235395	24.379742648051632	24.51957481569496	25.49949704301801
32	25.48015051565705	24.61947032598505	24.333139520655397	25.5672396377025
33	24.900084388185654	24.461603375527428	24.938649789029537	25.69966244725738
34	25.554899197461584	24.514806032118415	25.046202921543642	24.88409184887636
35	25.782406383808244	24.358184570775645	25.182827312788714	24.676581732627398
36	24.916076091010815	24.823924347807008	24.80569653046999	25.454303030712182
37	25.768748449338304	24.557924282138572	24.630161910178924	25.043165358344204
38	25.20297416968565	24.410317948562156	24.986012693638752	25.40069518811345
39	25.84990096971201	24.21689278652749	24.507527905275232	25.42567833848527
40	25.999449789958163	24.418102247928694	24.754726152360924	24.82772180975222
41	25.004662482668643	24.94888146829807	25.181457074357528	24.864998974675757
42	25.971444871284188	24.99468325715482	24.654411715063095	24.379460156497903
43	25.571099564454563	24.05854756863364	24.923562240126383	25.446790626785408
44	25.084433372322056	24.653363241566367	24.669481956322503	25.592721429789073
45	25.283615014401224	24.824278103477813	24.630433369986896	25.261673512134074
46	25.09618427548493	24.665538644601316	24.615494972425346	25.622782107488405
47	25.264159907779266	24.623560000438825	24.9557166642897	25.156563427492205
48	25.825259924385634	25.00751080205239	24.106130164731297	25.061099108830675
49	25.349040139616058	25.064136969561947	24.39196465040288	25.194858240419116
50	25.424814428500543	25.18261788305029	24.33163880140558	25.06092888704358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	87.0
2	44.0
3	83.0
4	122.0
5	145.5
6	169.0
7	136.0
8	103.0
9	141.0
10	179.0
11	302.5
12	426.0
13	785.0
14	1144.0
15	1492.0
16	1840.0
17	1844.5
18	1849.0
19	1752.0
20	1655.0
21	1939.5
22	2224.0
23	2797.5
24	3371.0
25	4450.0
26	5529.0
27	7138.0
28	8747.0
29	10612.5
30	12478.0
31	14456.0
32	16434.0
33	19108.5
34	21783.0
35	25770.0
36	29757.0
37	34895.5
38	40034.0
39	44141.0
40	48248.0
41	53264.0
42	58280.0
43	60791.0
44	63302.0
45	69496.5
46	75691.0
47	81429.0
48	87167.0
49	91212.5
50	95258.0
51	93729.0
52	92200.0
53	91981.5
54	91763.0
55	92411.5
56	93060.0
57	89440.0
58	85820.0
59	79145.0
60	72470.0
61	63128.5
62	53787.0
63	46510.0
64	39233.0
65	32795.5
66	26358.0
67	22447.0
68	18536.0
69	16199.5
70	13863.0
71	10762.5
72	7662.0
73	6817.0
74	5972.0
75	4399.0
76	2826.0
77	2491.0
78	2156.0
79	1827.5
80	1499.0
81	1132.0
82	765.0
83	669.5
84	574.0
85	442.0
86	310.0
87	204.0
88	98.0
89	64.0
90	30.0
91	21.0
92	12.0
93	10.0
94	8.0
95	11.5
96	15.0
97	14.0
98	13.0
99	11.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029704566148777683
2	0.00455695048873294
3	3.3755188805429184E-4
4	0.0
5	1.6877594402714592E-4
6	3.3755188805429184E-4
7	0.0
8	0.0
9	0.0
10	8.438797201357296E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.751037761085837E-4
17	0.0
18	2.531639160407189E-4
19	8.438797201357296E-5
20	3.3755188805429184E-4
21	1.6877594402714592E-4
22	0.0
23	8.438797201357296E-5
24	9.282676921493026E-4
25	3.3755188805429184E-4
26	4.219398600678648E-4
27	5.063278320814378E-4
28	0.0015189834962443132
29	7.594917481221566E-4
30	0.0011814316081900213
31	9.282676921493026E-4
32	8.438797201357295E-4
33	2.531639160407189E-4
34	0.0010970436361764486
35	4.219398600678648E-4
36	8.438797201357296E-5
37	0.002109699300339324
38	0.0
39	0.0010126556641628756
40	8.438797201357296E-5
41	0.0010126556641628756
42	0.005653994124909388
43	0.004388174544705794
44	0.003797458740610783
45	0.0028691910484614806
46	0.0032067429365157723
47	0.001687759440271459
48	0.0036286827965836374
49	0.0032911309085293457
50	0.0012658195802035944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1185003.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.03434600135058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97669046792872	46.812796155918484
2	13.785849742226116	16.552489466948646
3	3.9293830110643615	7.076938177741998
4	1.5502145505993847	3.7226446682804664
5	0.7790122601545889	2.3383745782707357
6	0.47011664062223435	1.6933887038464681
7	0.29153258854754455	1.2251377804072865
8	0.20490195527515703	0.9840923903473631
9	0.14692438011669426	0.7938458164763619
>10	0.7527589078160687	8.075078626977069
>50	0.05979736941720022	2.540901407185954
>100	0.047451584787195605	5.850649968899075
>500	0.004660417570431439	1.857942769742083
>1k	7.061238743077938E-4	0.47571948895787286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1218	0.10278454991253186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.6877594402714592E-4
6	0.0	0.0	0.0	0.0	2.531639160407189E-4
7	0.0	0.0	0.0	0.0	2.531639160407189E-4
8	0.0	0.0	0.0	0.0	2.531639160407189E-4
9	0.0	0.0	0.0	0.0	2.531639160407189E-4
10	0.0	0.0	0.0	0.0	2.531639160407189E-4
11	0.0	0.0	0.0	0.0	2.531639160407189E-4
12	0.0	0.0	0.0	0.0	4.2193986006786483E-4
13	0.0	0.0	0.0	0.0	5.907158040950107E-4
14	0.0	0.0	0.0	0.0	5.907158040950107E-4
15	0.0	0.0	0.0	0.0	6.751037761085837E-4
16	0.0	0.0	0.0	1.6877594402714592E-4	8.438797201357297E-4
17	0.0	0.0	0.0	2.531639160407189E-4	9.282676921493026E-4
18	0.0	0.0	0.0	2.531639160407189E-4	0.0010126556641628756
19	0.0	0.0	0.0	3.3755188805429184E-4	0.0010126556641628756
20	0.0	0.0	0.0	5.063278320814378E-4	0.0012658195802035944
21	0.0	0.0	0.0	5.907158040950107E-4	0.0012658195802035944
22	0.0	0.0	0.0	6.751037761085837E-4	0.0013502075522171674
23	0.0	0.0	0.0	9.282676921493026E-4	0.0013502075522171674
24	0.0	0.0	0.0	0.0012658195802035944	0.0014345955242307403
25	0.0	0.0	0.0	0.0016033714682578862	0.0014345955242307403
26	0.0	0.0	0.0	0.002362863216380043	0.0014345955242307403
27	0.0	0.0	0.0	0.0030379669924886265	0.0014345955242307403
28	0.0	0.0	0.0	0.0037130707685972104	0.0014345955242307403
29	0.0	0.0	0.0	0.004219398600678648	0.0014345955242307403
30	0.0	0.0	0.0	0.005822770068936534	0.0014345955242307403
31	0.0	0.0	0.0	0.008185633285316577	0.0015189834962443132
32	0.0	0.0	0.0	0.011561152165859496	0.0016033714682578862
33	0.0	0.0	0.0	0.013755239438212392	0.0016033714682578862
34	0.0	0.0	0.0	0.016624430486673874	0.0016877594402714593
35	0.0	0.0	0.0	0.02050627719929823	0.0016877594402714593
36	0.0	0.0	0.0	0.025316391604071887	0.0016877594402714593
37	0.0	1.6877594402714592E-4	0.0	0.03375518880542919	0.0016877594402714593
38	0.0	1.6877594402714592E-4	0.0	0.04405052139108508	0.0016877594402714593
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	730	0.0	28.631866	1
GTATCAA	1520	0.0	26.198952	1
GTCCTAA	205	0.0	18.245005	1
GCTAGAC	85	1.4310655E-4	18.117212	3
TCTATTC	125	4.8535003E-7	17.599577	3
TATACTG	165	1.6589183E-9	17.332918	5
GTCCTAT	305	0.0	17.312523	1
CTTACAC	320	0.0	17.187088	3
GTCCTAC	360	0.0	17.112146	1
ATCAACG	2350	0.0	16.944275	3
CGCAATA	260	0.0	16.924099	36
AGTGATC	145	1.2495002E-7	16.689257	8
TAGGACG	185	4.2200554E-10	16.648249	4
TCAACGC	2385	0.0	16.603376	4
CTATTCC	320	0.0	16.499605	4
CAACGCA	2435	0.0	16.262444	5
GTGTATA	325	0.0	16.247135	1
CGGATCG	95	3.3457793E-4	16.210821	26
CCCGTAC	95	3.346855E-4	16.210138	3
AACGCAG	2465	0.0	16.064524	6
>>END_MODULE