FastQCFastQC Report
Thu 2 Feb 2017
SRR4061951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660252
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11080.16781471317012292No Hit
TATCAACGCAGAGTACTTTTTTTTT7440.1126842478326457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.016.4876691
CCCTATA653.3684992E-614.6139272
GCCGTCC400.0052703714.251828
TTATACA602.5625835E-514.2507394
TAAGGGA801.993265E-613.0631784
AGGACCG604.084686E-412.6673235
GTAAGGG604.084686E-412.6673233
CCCTTAT604.092535E-412.6644421
GTATCAA8000.012.5852891
GTATAAT853.941326E-612.291961
GACTGTC701.0877658E-412.2158467
TCTATAC701.0885365E-412.2149193
GTGTAAT701.0908515E-412.2121411
TGGACTG1251.4024408E-912.160635
AACAGGT550.003062292212.0924547
CTTCGCC550.00306577412.0906216
CTTAACA550.003067516312.0897032
GTCTTAG802.8656477E-511.8729141
CCTATAG1052.7086935E-711.7625153
TTTATAC658.009184E-411.6929153
Produced by FastQC (version 0.11.5)