##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061951_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660252
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3085715757014	32.0	32.0	32.0	32.0	32.0
2	30.91880221491188	32.0	32.0	32.0	32.0	32.0
3	30.927950237182166	32.0	32.0	32.0	32.0	32.0
4	30.933587478720245	32.0	32.0	32.0	32.0	32.0
5	30.888603139407376	32.0	32.0	32.0	32.0	32.0
6	34.53640428200142	36.0	36.0	36.0	32.0	36.0
7	34.48280505019296	36.0	36.0	36.0	32.0	36.0
8	34.44578585146277	36.0	36.0	36.0	32.0	36.0
9	34.53866553982419	36.0	36.0	36.0	32.0	36.0
10	34.29803771893156	36.0	36.0	36.0	32.0	36.0
11	34.53068525350927	36.0	36.0	36.0	32.0	36.0
12	34.39835850554031	36.0	36.0	36.0	32.0	36.0
13	34.459865324148964	36.0	36.0	36.0	32.0	36.0
14	34.3745433561731	36.0	36.0	36.0	32.0	36.0
15	34.339091740729295	36.0	36.0	36.0	32.0	36.0
16	34.33391038573151	36.0	36.0	36.0	32.0	36.0
17	34.31533414514458	36.0	36.0	36.0	32.0	36.0
18	34.28869128756899	36.0	36.0	36.0	32.0	36.0
19	34.301425819232655	36.0	36.0	36.0	32.0	36.0
20	34.28743570636666	36.0	36.0	36.0	32.0	36.0
21	34.277403173333816	36.0	36.0	36.0	32.0	36.0
22	34.231847839915666	36.0	36.0	36.0	32.0	36.0
23	34.210939156564464	36.0	36.0	36.0	32.0	36.0
24	34.18381920842345	36.0	36.0	36.0	32.0	36.0
25	33.841986090159516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	9.0
5	48.0
6	126.0
7	31.0
8	100.0
9	87.0
10	69.0
11	12.0
12	32.0
13	35.0
14	92.0
15	127.0
16	198.0
17	256.0
18	379.0
19	545.0
20	766.0
21	1012.0
22	1618.0
23	2294.0
24	3319.0
25	4684.0
26	6521.0
27	8782.0
28	11590.0
29	15621.0
30	20626.0
31	28547.0
32	41150.0
33	59401.0
34	136661.0
35	315514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4325969905111	17.726752947769388	12.417360222341706	25.423289839377805
2	16.262578530621642	20.043559125909503	38.13301587878568	25.56084646468318
3	18.624393598153418	24.075838284763126	28.940835778125457	28.358932338958
4	12.931619478088061	16.280514035395978	35.67574690845638	35.112119578059584
5	14.479594820415937	36.551610249479964	34.0543865416868	14.914408388417295
6	33.17436214515631	35.51301169324774	17.400449786927084	13.912176374668872
7	29.617174826023014	30.802947374804795	21.001410009551677	18.578467789620507
8	27.39685942619409	33.96380319636849	19.48220103978252	19.157136337654894
9	26.894610827473997	15.506096386747922	18.9317789985883	38.667513787189776
10	16.28078029575775	27.31960248168386	31.695458385085633	24.704158837472757
11	35.44049796609244	22.428494726400757	22.768389345418473	19.362617962088326
12	23.95692890995261	24.431469194312797	29.10240758293839	22.509194312796208
13	28.795973486152416	20.762356523598445	25.50479278284698	24.936877207402162
14	23.400677484996162	19.926064976694395	25.86073043436068	30.81252710394876
15	24.891870086382607	27.379161409298263	22.620990245559547	25.107978258759584
16	24.77527203911055	26.523813278662672	24.110034816153725	24.590879866073053
17	23.500154670128044	26.88439772665239	25.42246174188892	24.192985861330648
18	23.530303673558507	26.724880368265598	26.20056283016236	23.544253128013537
19	24.907406986281224	25.92270852189258	25.886626430586695	23.283258061239493
20	25.437097788928725	25.410264767636647	25.344622404814782	23.808015038619843
21	25.195287925574572	25.767697944569	25.33672292232293	23.700291207533496
22	25.299118865258546	25.16009605763458	25.672524726957384	23.868260350149484
23	24.593014090655544	25.662091043581597	25.674823563650474	24.070071302112385
24	24.270437601001753	25.70515522055903	25.66149517465534	24.362912003783872
25	24.596433730848698	25.346493229303253	25.728192868977583	24.328880170870466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	40.0
2	121.0
3	202.0
4	202.0
5	202.0
6	382.5
7	563.0
8	563.0
9	563.0
10	811.0
11	1059.0
12	1059.0
13	1059.0
14	1545.0
15	2031.0
16	2031.0
17	2031.0
18	3718.0
19	5405.0
20	5405.0
21	5405.0
22	8053.0
23	10701.0
24	10701.0
25	10701.0
26	15111.5
27	19522.0
28	19522.0
29	19522.0
30	25655.5
31	31789.0
32	31789.0
33	31789.0
34	39231.5
35	46674.0
36	46674.0
37	46674.0
38	54736.5
39	62799.0
40	62799.0
41	62799.0
42	68966.0
43	75133.0
44	75133.0
45	75133.0
46	80322.5
47	85512.0
48	85512.0
49	85512.0
50	86461.0
51	87410.0
52	87410.0
53	87410.0
54	82813.0
55	78216.0
56	78216.0
57	78216.0
58	69286.5
59	60357.0
60	60357.0
61	60357.0
62	51402.0
63	42447.0
64	42447.0
65	42447.0
66	34485.5
67	26524.0
68	26524.0
69	26524.0
70	19650.0
71	12776.0
72	12776.0
73	12776.0
74	9644.5
75	6513.0
76	6513.0
77	6513.0
78	4668.5
79	2824.0
80	2824.0
81	2824.0
82	1886.0
83	948.0
84	948.0
85	948.0
86	658.0
87	368.0
88	368.0
89	368.0
90	271.0
91	174.0
92	174.0
93	174.0
94	122.0
95	70.0
96	70.0
97	70.0
98	132.5
99	195.0
100	195.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010602012564899462
2	0.0
3	7.57286611778533E-4
4	0.009693268630765223
5	0.030140007148785616
6	0.058159611784591336
7	0.1032938938465919
8	0.10465700974779328
9	0.11556193695740413
10	0.1304047545482634
11	0.14085530979080715
12	0.1328280717059547
13	0.12464937629874653
14	0.1141988210562028
15	0.13025329722590767
16	0.11934837001629682
17	0.11874254072687397
18	0.1102609306749544
19	0.09768997291943077
20	0.09344916789347098
21	0.08860253357808837
22	0.09814434488649788
23	0.07936363691439026
24	0.09238896663698104
25	0.08648213106510846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	660252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.709367176894375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.9136982396115	17.961923187606306
2	15.333638876532573	10.03107248340812
3	8.226255945978304	8.072268786843447
4	5.23349997679204	6.847378894446361
5	3.5661669814649217	5.83235326054266
6	2.491039897093302	4.888820318779079
7	1.8670181055248747	4.2748286517666
8	1.4938363450457415	3.908995320983282
9	1.0834761830429065	3.18958382687134
>10	5.637457172258074	30.613174816733302
>50	0.12926771292679506	2.781529199605092
>100	0.023714580192010663	1.3163897106354794
>500	4.6499176847079724E-4	0.11315932347923705
>1k	4.6499176847079724E-4	0.16852221829972397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1108	0.16781471317012292	No Hit
TATCAACGCAGAGTACTTTTTTTTT	744	0.1126842478326457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	265	0.0	16.487669	1
CCCTATA	65	3.3684992E-6	14.613927	2
GCCGTCC	40	0.00527037	14.25182	8
TTATACA	60	2.5625835E-5	14.250739	4
TAAGGGA	80	1.993265E-6	13.063178	4
AGGACCG	60	4.084686E-4	12.667323	5
GTAAGGG	60	4.084686E-4	12.667323	3
CCCTTAT	60	4.092535E-4	12.664442	1
GTATCAA	800	0.0	12.585289	1
GTATAAT	85	3.941326E-6	12.29196	1
GACTGTC	70	1.0877658E-4	12.215846	7
TCTATAC	70	1.0885365E-4	12.214919	3
GTGTAAT	70	1.0908515E-4	12.212141	1
TGGACTG	125	1.4024408E-9	12.16063	5
AACAGGT	55	0.0030622922	12.092454	7
CTTCGCC	55	0.003065774	12.09062	16
CTTAACA	55	0.0030675163	12.089703	2
GTCTTAG	80	2.8656477E-5	11.872914	1
CCTATAG	105	2.7086935E-7	11.762515	3
TTTATAC	65	8.009184E-4	11.692915	3
>>END_MODULE